Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Ruiz-Linares, Matilde; Baca, Pilar; Arias-Moliz, María Teresa; Ternero, Francisco Jesús; Rodríguez, Jesús Israel; Ferrer-Luque, Carmen Marı́a

doi:10.4012/dmj.2018-090

Cited by 18 publications

(12 citation statements)

References 30 publications

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“…No significant difference between the four groups was observed after 48 h. However, the overall antimicrobial efficacy of all four RCS is to be rated as moderate or low, considering that the mean CFU/ml dropped by just 0.5–1 log-scales. These findings are also consistent with data from previous studies, although none of these studies assessed the antibacterial effects using multi-species bacterial suspensions [ 30 , 44 , 45 ]. Furthermore, there is evidence from our in vitro results that the antimicrobial efficacy of the RCS may be different depending on the bacterial species.…”

Section: Discussionsupporting

confidence: 92%

A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers

et al. 2022

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Objectives To evaluate the biological and physicochemical features of bioactive root canal sealers. Materials and methods Human periodontal ligament fibroblasts (hPDLF) and human osteoblasts (hOB) were exposed to eluates of three bioactive root canal sealers, GuttaFlow® bioseal (GF), BioRoot™ RCS (BR), and TotalFill® BC Sealer (TF), and the epoxy resin–based sealer AH plus® (AH). Cytotoxicity and cellular inflammatory response were evaluated. The osteogenic potential was examined using human mesenchymal stem cells (hMSC). Film thickness, flowability, and pH were assessed. Root canal treatment was performed on human extracted teeth to evaluate the sealers’ tightness towards bacterial penetration. The antibacterial activity against common pathogens in primary root canal infections was tested. Results AH was severely cytotoxic to hPDLF and hOB (p < 0.001). The bioactive sealers were generally less cytotoxic. IL-6 levels in hPDLF were elevated in the presence of AH (p < 0.05). AH and GF suppressed IL-6 production in hOB (p < 0.05). AH and BR stimulated the PGE2 production in hPDLF and hOB (p < 0.05). BR was the only sealer that led to calcium deposits in hMSC (p < 0.05). TF and AH showed the lowest film thickness and the highest flowability. Bacterial tightness was best in teeth filled with AH and BR. All sealers showed similar antimicrobial activity, but the overall antimicrobial efficacy was moderate as the bacteria were reduced by just one log scale (p < 0.05). Conclusions This study revealed favorable in vitro results regarding the biocompatibility of the bioactive root canal sealers. Clinical relevance Bioactive root canal sealers may be a useful alternative to epoxy resin–based sealers.

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Section: Discussionsupporting

confidence: 92%

A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers

et al. 2022

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“…We believe that since the catalyst part of the epoxy resin contains amines, oxidation of PY could have activated the polymerization and/or reduced beneficial effects of the phenolic compounds via formation of covalent bonds with the amines [23]. One of the first outcomes obtained in this study was that the control (free of polyphenols) sealer had some antibacterial effects against E. faecalis after 24 h. Ruiz-Linares et al [24] analyzed an epoxy resin-based sealer (AH-Plus, Dentsply Sirona, Konstanz, Germany) against E. faecalis and they showed that such material could induce some antibacterial effects as a result of the release of formaldehyde and/or other toxic non-polymerized components, such as amines or epoxy monomers.…”

Section: Discussionmentioning

confidence: 64%

Impacts of Resveratrol and Pyrogallol on Physicochemical, Mechanical and Biological Properties of Epoxy-Resin Sealers

et al. 2022

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This study aimed at evaluating the physicochemical and biological properties of experimental epoxy-resin sealers containing polyphenols such as resveratrol and pyrogallol. A conventional epoxy resin (OB) was modified by adding different concentrations of resveratrol (RS) or pyrogallol (PY) to its composition. Antibacterial and antioxidant activities, mechanical properties, along with wettability and morphological changes were investigated. The results were statistically analyzed using ANOVA and multiple comparison tests (α = 0.05). The incorporation of the tested polyphenols into the epoxy resin enhanced its mechanical properties. PY demonstrated much better antioxidant and antibacterial activities than RS, which were associated with a higher release of PY. In contrast, PY showed a higher cytotoxicity than OB and OB doped with RS. OB containing PY presented a rougher surface and higher water absorption than OB doped with RS. Both tested polyphenols caused no notable changes to the overall porosity of OB. Resveratrol and pyrogallol may not only influence the morphology and mechanical properties of epoxy-resin sealers, but could also enhance antioxidant activity and antibacterial effects against Enterococcus faecalis. Most epoxy-resin sealers currently available in the market can be considered as “passive” materials. Thus, doping their composition with specific polyphenols may be a suitable strategy to confer some antibacterial properties, antioxidant potential, along with improvement of some mechanical properties.

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“…In recent years, interest in endodontic Re-treatment has been increasing with the extension of the human lifespan and advancements in endodontics. Classically, AH Plus was considered a “gold standard” sealer because of its satisfactory properties for clinical usage; it has been widely used in endodontics [ 23 , 24 , 25 ]. However, calcium silicate-based sealers have recently been introduced and are gaining popularity.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Obturation Quality between Calcium Silicate-Based Sealers and Resin-Based Sealers for Endodontic Re-treatment

2021

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Background: The objective of this micro-computed tomography (micro-CT)-based study was to compare the filling quality of endodontic treatment and endodontic Re-treatment between two sealers with matched obturation techniques: calcium silicate-based sealer (Endoseal TCS) with a single-cone technique (SCT) and resin-based sealer (AH Plus) with a continuous wave technique (CWT). Methods: Forty maxillary premolars were selected and assigned into four groups, according to the obturation methods of the first endodontic treatment and Re-treatment (n = 10). The AP/AP group was first treated with AH Plus/CWT, then re-treated with AH Plus/CWT. The AP/ET group was first treated with AH Plus/CWT, then re-treated with Endoseal TCS/SCT. The ET/AP group was first treated with Endoseal TCS/SCT, then re-treated with AH Plus/CWT, and the ET/ET group was first treated with Endoseal TCS/SCT, then re-treated with Endoseal TCS/SCT. The specimens were scanned using micro-CT at three time points: after the first endodontic treatment, after gutta-percha (GP) cone removal, and after Re-treatment. The void volume of root canal obturation and the volume of the remaining filling materials were calculated. Data were analyzed using Student’s t-tests and ANOVA. Results: The Endoseal TCS groups (ET/AP and ET/ET) showed a lower percentage of voids than the AH plus groups (AP/AP and AP/ET) on the whole canal and the apical third, after first obturation (p < 0.05). The AH plus group showed significantly fewer remnants than the Endoseal TCS group after GP removal (p < 0.05). Re-treated canals and initially treated canals had similar void volumes (p > 0.05). There was no significant difference in void volume after Re-treatment, regardless of whether the same or different sealers were used for the first treatment and Re-treatment (p < 0.05). Conclusions: Endoseal TCS sealer and AH Plus sealer had a similar Re-treatment efficacy, regardless of which sealer was used in the previous treatment.

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Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Cited by 18 publications

References 30 publications

A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers

A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers

Impacts of Resveratrol and Pyrogallol on Physicochemical, Mechanical and Biological Properties of Epoxy-Resin Sealers

Comparison of Obturation Quality between Calcium Silicate-Based Sealers and Resin-Based Sealers for Endodontic Re-treatment

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