Antibacterial Properties of Nutmeg Oil in Pork and Its Possible Mechanism

Abstract

The aim of this study is to investigate the antibacterial characteristics and mechanisms of nutmeg oil. In this paper, 54 chemical compositions of nutmeg oil were analyzed by gas chromatography–mass spectrometry (GC–MS). The major volatile components of nutmeg oil were sabinene (39.12%) and alpha‐pinene (11.96%). In addition, nutmeg oil showed significant antibacterial activity, minimum inhibitory concentration, and minimum bactericide concentration of seven pathogens are 0.05 and 0.1%, respectively, and the nutmeg oil also had a significant effect on inhibiting the growth of Escherichia coli and Staphyloccocus aureus in phosphate buffer saline and pork. Subsequently, in order to reveal the possible antibacterial mechanisms of nutmeg oil against pathogens, the damage of cell membrane was observed by scanning electron microscopy, the loss of 260 nm absorbing material was tested by ultraviolet spectrophotometer, and the cellular adenosine triphosphate concentrations were measured by the Surface Hygiene Test Kit. Finally, the decrease of nucleic acid was evaluated by inverted fluorescence microscope. These results indicated that the antibacterial mechanisms of the nutmeg oil against bacteria might be interpreted as disrupting cell membrane and inhibiting synthesis of DNA. Practical Applications As a natural and safe antibacterial agent, nutmeg oil exhibited good antimicrobial activity against a wide range of spoilage and pathogenic bacteria. Meanwhile, the antimicrobial activity of nutmeg oil has been proved on pork. Based on the results, we believe that nutmeg oil can be applied in meat storage and preservation.

Antimicrobial effect of black pepper petroleum ether extract for the morphology of Listeria monocytogenes and Salmonella typhimurium

“…These results were consistent with the earlier findings (Cui et al 2015), indicating that the nutmeg oil in pork could destroy the cell wall and cell membrane of E. coli and S. aureus. The leakage of ALP-ase could degrade the main nucleic acid components, such as RNA and DNA.…”

The Essential Oil and Hydrolats from Myristica fragrans Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity

“…The essential oil EO2 investigated in this study has a similar composition, meaning it could also be efficient against these pathogens at higher concentrations. In another study, the nutmeg essential oil with similar quantity of volatile compounds had a significant effect on the inhibition of the growth of E. coli and S. aureus [34]. In this study, we found that essential oil EA2 (0.5%) inhibited E. faecalis.…”

Essential Oil Composition, Antioxidant and Antibacterial Activities of Nutmeg (Myristica fragrans Houtt) from Garut West Java

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