Antibacterial properties of Ipomoea staphylina Roem & Schult. plant extracts with comparing its preliminary qualitative phytochemical and quantitative GC-MS analysis

Abstract

Ipomoea staphylina plant ethanolic extract was subjected to preliminary qualitative phytochemical and quantitative GC-MS analysis with analyzing its antibacterial activity by the standard method. The preliminary qualitative phytochemical analysis is done by standard procedure and quantitative GCMS analysis is done by subjecting it to GC Model: Thermo Trace GC Ultra model instrument. Antibacterial activity is done by the standard agar well diffusion method. Preliminary qualitative phytochemical analysis for pet ether and chloroform extract confirms for fewer phytochemicals but ethanolic extract confirms for the presence of alkaloids, saponins, flavonoids, steroids, glycosides, phenols and sterols. GC-MS analysis of I. staphylina ethanolic leaf extract confirms the presence of 79 compounds, out of these 24 compounds were unknown and 55 compounds were known for its medicinal properties. The antibacterial experiment revealed that I. staphylina plant ethanolic extract has appreciable antibacterial activity in all tested concentrations against selected bacterial pathogens but comparably less with the standard ciprofloxacin used but pet ether and chloroform extract showed negligible antibacterial activity. I. staphylina plant could be exploited as a valuable source of antibacterial agent enriching with known antibacterial compounds.

Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G. Don. by GCMS Analysis

“…The compounds were identi ed based on the mass fragmentation pattern and comparing the peak area and retention time of the NIST database. The chemical composition of the crude methanol extract is summarized in Table 1, with the most abundant compound being 5-Hydroxymethylfurfural (11.11%) used as Antioxidant agent (Shapla et al, 2018), followed by 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-(7.41%) used as Antimicrobial, anti-in ammatory, antioxidant agent (Padmashree et al, 2018), and Heptanoic acid (7.41%) of unknown medicinal property (Table 1). The detailed mass spectra of the twenty three compounds identi ed by GC-MS analysis of A. nobilis root are shown in Figs.…”

Potensi Ekstrak Ethanol Daun Salam Dan Air Rebusan Daun Salam Untuk Memperbaiki Profil Lipid (Ldl-Kolesterol) Darah Pada Tikus Wistar

“…Alkaloid, flavonoid, saponin, tannin, dan minyak atsiri merupakan senyawa umum yang dapat diekstrak dari simplisia bahan alam (berbagai jenis tanaman). Irmadoly et al (2014), Padmashree et al (2018) dan Astiti et al (2018), melaporkan bahwa golongan senyawa-senyawa kimia tersebut mempunyai efek menurunkan kadar LDL kolesterol darah atau sering disebut dengan antihiperkolesterolemia.…”

In vitro antimutagenic activity of Ipomoea staphylina

“…Ipomoea staphylina leaves extract possesses mainly antiinflammatory (Firdous and Koneri, 2013), antiulcer (Banerjee and Firdous, 2015), anti-diabetic (Firdous and Singh, 2016), hepatoprotective, nephroprotective (Bag and Mumtaz, 2013), cytotoxic (Padmashree et al, 2018a) and antibacterial (Padmashree et al, 2018b) activities. There is no report on the antimutagenic activity of this plant.…”