Antibacterial and Anticancer activity of ε ‐poly‐L‐lysine (ε ‐PL) produced by a marine Bacillus subtilis sp.

El‐Sersy, Nermeen A.; Abdelwahab, Abeer E.; Abouelkhiir, Samia S.; Abou‐Zeid, Dunja‐Manal; Sabry, Soraya A.

doi:10.1002/jobm.201100290

Cited by 84 publications

(43 citation statements)

References 28 publications

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“…Pokrovsky reported that L-Lysine α-oxidase showed considerable cytotoxicity against seven tumor cell lines [28]. El-Sersy also isolated a poly-lysine component which inhibited three human cell lines (Hela S3, HepG2 and CaCo) [29]. Our investigation demonstrated that J2-C3 had a higher level of lysine, which might be related to the anti-tumor activity, but its mechanism needs to be studied further.…”

Section: Discussionmentioning

confidence: 64%

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

Chen

Song

et al. 2013

Marine Drugs

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A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native-PAGE). The purity of J2-C3 was over 99% in reversed phase-high performance liquid chromatography (RP-HPLC). The molecular weight was determined as 20,538.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). J2-C3 was rich in Glx (Gln + Glu), Lys, and Asx (Asp + Asn) according to amino acid analysis. Four partial amino acid sequences of this peptide were determined as L/ISMEDVEESR, KNGMHSI/LDVNHDGR, AMKI/LI/LNPKKGI/LVPR and AMGAHKPPKGNEL/IGHR via MALDI-TOF/TOF-MS and de novo sequencing. Secondary structural analysis by CD spectroscopy revealed that J2-C3 had the α-helix (45.2%), β-sheet (2.9%), β-turn (26.0%) and random coil (25.9%). The anti-tumor effect of J2-C3 against human tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC50 values of J2-C3 were 65.57, 93.33 and 122.95 µg/mL against A549, HT-29 and HepG2 cell lines, respectively. Therefore, J2-C3 might be developed as a potential anti-tumor agent.

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Section: Discussionmentioning

confidence: 64%

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

Chen

Song

et al. 2013

Marine Drugs

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“…Fractions of viable cells were measured with MTT assay. [33] The inhibition of growth rate of tumour cells for each compound with different concentrations was calculated as follows: [100 ¡ (absorbance of cells that were exposed to the compound at different concentrations/absorbance of control well)] £ 100.…”

Section: ¡1mentioning

confidence: 99%

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Abd-Elnaby

Abo-Elala

Abdel-Raouf

et al. 2015

Biotechnology & Biotechnological Equipment

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A total of 17 actinomycetes were isolated and screened against five bacterial pathogens. Forty-one per cent of the isolates were active against the tested pathogens. The most potent isolate was identified as Streptomyces parvus by using a 16S rRNA sequence analysis. S. parvus produced active compound(s) against a number of Gram negative and Gram positive bacteria. The obtained inhibition zones were 14, 19, 20 and 20 mm against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Aeromonas hydrophila, respectively. Moreover, the anticancer activity of S. parvus was tested against four different cell lines: human liver cancer cell line, mouse lymphoma cell line, breast cancer cell line and human colon cancer cell line. The inhibition activities were 53%, 56%, 57% and 42%, respectively. To achieve a maximum production of the bioactive compound, PlackettÀBurman design was applied. The productivity increased up to 1.3-fold, when S. parvus was grown in optimized medium composed of: 10 g, 2 mL (10 3 colony-forming units mL ¡1 ) inoculum size with pH 8 for 7 d of incubation. The main constitutes of S. parvus crude extract were determined by gasÀliquid chromatography mass spectrometry. They were found to be ethane, 1,1-diethoxy; di-n-octyl phthalate; ethanol, 2,2-diethoxy; 9,12-octadecadienoic acid; methyl ester (E,E) and benzoic acid.

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“…Anticancer ε -poly-L-lysine (ε -PL) was produced by a marine Bacillus subtilis sp. isolated from sea water in Alexandria [26]. We isolated a strain, S-1, from the bottom sediments of South China Sea.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Marine Brevibacillus sp. S-1 Collected from South China Sea and a Novel Antitumor Peptide Produced by the Strain

et al. 2014

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A Gram-positive, rod-shaped bacterium, designated as S-1, was isolated from a marine sediment sample collected from South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence showed that S-1 belongs to the genus Brevibacillus. A novel cytotoxic peptide was isolated from the fermentation broth of the marine-derived bacterium Brevibacillus sp. S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography. The molecular weight of this peptide was determined as 1570 Da by MALDI-TOF mass spectrometry, and its structure was proposed as a cyclic peptide elucidated by MALDI-TOF/TOF mass spectrometry and de novo sequencing. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this peptide exhibited cytotoxicity against BEL-7402 human hepatocellular carcinoma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, U251 human glioma cells and MCF-7 human breast carcinoma cells. Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells. The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

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Antibacterial and Anticancer activity of ε ‐poly‐L‐lysine (ε ‐PL) produced by a marine Bacillus subtilis sp.

Cited by 84 publications

References 28 publications

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Isolation and Characterization of Marine Brevibacillus sp. S-1 Collected from South China Sea and a Novel Antitumor Peptide Produced by the Strain

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