Isolation and Characterization of Marine Brevibacillus sp. S-1 Collected from South China Sea and a Novel Antitumor Peptide Produced by the Strain

Abstract

A Gram-positive, rod-shaped bacterium, designated as S-1, was isolated from a marine sediment sample collected from South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence showed that S-1 belongs to the genus Brevibacillus. A novel cytotoxic peptide was isolated from the fermentation broth of the marine-derived bacterium Brevibacillus sp. S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography. The molecular weight of this peptide was determined as 1570 Da by MALDI-TOF mass spectrometry, and its structure was proposed as a cyclic peptide elucidated by MALDI-TOF/TOF mass spectrometry and de novo sequencing. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this peptide exhibited cytotoxicity against BEL-7402 human hepatocellular carcinoma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, U251 human glioma cells and MCF-7 human breast carcinoma cells. Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells. The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

PBN11-8, a Cytotoxic Polypeptide Purified from Marine Bacillus, Suppresses Invasion and Migration of Human Hepatocellular Carcinoma Cells by Targeting Focal Adhesion Kinase Pathways

“…A novel bioactive peptide, SBP, was isolated through the fermentation of the marine Brevibacillus sp. S-1 by our research group, and it demonstrates wide antitumor activity [4]. An extracellular l -asparaginase is produced by a protease-deficient isolate, Bacillus aryabhattai ITBHU02, and in vitro cytotoxicity assays with HL60 and MOLT-4 cell lines indicate that the enzyme has significant antineoplastic properties [5].…”

Genomic, biochemical, and phylogenetic evaluation of bacteria isolated from deep-sea sediment harboring methane hydrates

“…From rich-media cultures we obtained 40 isolates of Bacillales, including the genus Bacillus, Brevibacillus and Paenibacillus, all with known marine diversity (Ettoumi et al 2013;Lee et al 2013;Jhala et al 2014;Zheng et al 2014). These isolates were obtained from site PC44, located within a pockmark with thriving benthic fauna, including Acharax sp.…”

Identification of the Key Fields and Their Key Technical Points of Oncology by Patent Analysis

“…Natural products cover a variety of species distributed from land to ocean, and medicinal plants, Chinese herbal medicine and marine organisms, especially marine organisms, are important sources for natural products. With marine organisms living in special environment, a large amount of substances with unique and novel structures as well as special biological activity are accumulated in the process of growing and metabolism [ 28 , 29 ]. Therefore, marine natural products are important resources for novel marine drugs, which will be effective for many kinds of diseases.…”