Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Abstract

A total of 17 actinomycetes were isolated and screened against five bacterial pathogens. Forty-one per cent of the isolates were active against the tested pathogens. The most potent isolate was identified as Streptomyces parvus by using a 16S rRNA sequence analysis. S. parvus produced active compound(s) against a number of Gram negative and Gram positive bacteria. The obtained inhibition zones were 14, 19, 20 and 20 mm against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Aeromonas hydrophila, respectively. Moreover, the anticancer activity of S. parvus was tested against four different cell lines: human liver cancer cell line, mouse lymphoma cell line, breast cancer cell line and human colon cancer cell line. The inhibition activities were 53%, 56%, 57% and 42%, respectively. To achieve a maximum production of the bioactive compound, PlackettÀBurman design was applied. The productivity increased up to 1.3-fold, when S. parvus was grown in optimized medium composed of: 10 g, 2 mL (10 3 colony-forming units mL ¡1 ) inoculum size with pH 8 for 7 d of incubation. The main constitutes of S. parvus crude extract were determined by gasÀliquid chromatography mass spectrometry. They were found to be ethane, 1,1-diethoxy; di-n-octyl phthalate; ethanol, 2,2-diethoxy; 9,12-octadecadienoic acid; methyl ester (E,E) and benzoic acid.

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

“…Elnaby et al . (61) tested the antiviral activity of di-n-octyl phthalate isolated from a marine actinomycetes isolate ( S. parvus ) against hepatitis C virus (HCV). Compounds produced by Pseudoalteromonas piscida contained benzene 1-pentyloctyl, benzene 1-butylheptyl and di-n-octyl phthalate, and these compounds were found to be active against HCV (62).…”

Investigation of antibacterial and anti-cancer activities of Streptomyces sp SRF1 culture filtrate

“…Several cancer cell lines were shown to be susceptible including MCF-7. S. parvus extract has also been shown to be active against breast cancer cell lines [28]. Isolation and structure elucidation of the compounds responsible for the bioactivities of Streptomyces sp.…”

Water electrode plasma discharge to enhance the bacterial inactivation in water

“…Water contamination due to pathogenic microorganisms and invasive species has become a global concern. Such contamination of water has a harmful influence not only on the environment, but also on human health [1,2]. Moreover, it has been reported as a critical issue in seawater that 7000 or more different microbial species are annually transported through ballast water, resulting in destruction and contamination of marine ecosystems [3][4][5].…”