Antibacterial and anti-biofilm activity of cinnamon essential oil and eugenol

Millezi, Alessandra Farias; Costa, Karine Angélica Dalla; Oliveira, J. M.; Lopes, Susana Patrícia; Píccoli, Roberta Hilsdorf

doi:10.1590/0103-8478cr20180314

Cited by 20 publications

(11 citation statements)

References 20 publications

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“…It has been reported that oregano oil at 0.1% (v/v) inhibited biofilm formation of E. coli (Oral et al 2010). The Cinnamomum zeylanicum EO inhibited biofilm formation at inhibitory concentration of 1.92% (v/v) which was the most satisfactorily with E. coli reduction of biomass (Millezi et al 2019). The essential oil of another cinnamon species (Cinnamomum burmannii) was able to inhibit biofilm formation of S. aureus and P. aeruginosa by 50% at concentration of 0.03% (v/v) and destabilized biofilms of both bacteria at a concentration of 0.12% (v/v) (Pratiwi et al 2015).…”

Section: Inhibition Of Initial Bacteria Cell Attachment (Biofilm Inhibition)mentioning

confidence: 91%

Decoding antibacterial and antibiofilm properties of cinnamon and cardamom essential oils: a combined molecular docking and experimental study

2021

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This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body. Graphical Abstract

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Section: Inhibition Of Initial Bacteria Cell Attachment (Biofilm Inhibition)mentioning

confidence: 91%

Decoding antibacterial and antibiofilm properties of cinnamon and cardamom essential oils: a combined molecular docking and experimental study

2021

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“…flexuosus EOs on the formation of individual biofilms, the perspective of the action of these oils against co-cultivation, the biofilms formed together of a Gram negative bacterial species (P. aeruginosa) and Gram positive (S. aureus) (NOSTRO et al, 2007, ADUKWU et al, 2012MILLEZI et al 2019).…”

Section: Resultsmentioning

confidence: 99%

Anti-biofilm property of essential oils from Cymbopogon sp. against pathogenic bacteria in single-culture and co-culture

Millezi

Schuh²,

Schuh³

et al. 2020

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In this study, we evaluated whether the essential oils (EOs) from Cymbopogon flexuosus and Cymbopogon martinii can prevent production of biofilms either in single or combined culture of Staphylococcus aureus and Pseudomonas aeruginosa. Biofilm formation was assessed by microtiter-plate test with further quantification of viable cells and biofilm biomass. The evaluated EOs at 0.78 % significantly (P 0.05) reduced only the viable cells of S. aureus that inhabited biofilm. However, in single-and co-culture assays, both oils significantly (P 0.05) decreased the amount of biofilm biomass. Biofilm reductions between 52-83% and 60-93% were achieved for the treatments with EOs from C. flexuosus and C. martinii, respectively. Although the biomass reductions of simgle and co-cultivated biofilms were significant, the same was not true for viable cells, except for S. aureus. Considering that the remaining colony forming units can reconstitute the EPS matrix, studies with higher concentrations than those used in this research are suggested in order to obtain greater logarithmic reductions of viable bacterial cells.

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“…A fresh medium containing EO concentrations corresponding to the MICx3 and MICx5 values was added. The plates were incubated for 24 h at 37 °C [ 45 , 46 ]. The slime production test was used to evaluate the adhered biomass.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition, Antipathogenic and Cytotoxic Activity of the Essential Oil Extracted from Amorpha fruticosa Fruits

et al. 2021

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The purpose of this paper was to characterize and investigate the antimicrobial potential of Amorpha fruticosa fruits essential oil (EO). The EO was extracted by hydrodistillation, analyzed by GC-MS, and then evaluated for its interaction with microbial and mammalian cells. The antimicrobial activity was assessed against bacterial and fungal strains, in a planktonic and adherent growth state, using qualitative and quantitative assays. The main components identified in A. fruticosa fruits EO were δ-cadinene, γ-muurolene, and α-muurolene. The Gram-positive strains proved to be more susceptible than Gram-negative bacteria and fungal strains. The EO exhibited good antibiofilm activity, inhibiting the microbial adherence to the inert (96-well plates and Foley catheter section) and cellular substrata. The flow cytometry analysis revealed as one of the possible mechanisms of antimicrobial action the alteration of cell membrane hydrophobicity. The cytotoxicity on the L929 cell line occurred at concentrations higher than 0.3 mg/mL. Taken together, our results demonstrate that A. fruticosa fruits EO contains active compounds with selective inhibitory effect on different microbial strains in planktonic and biofilm growth state, explained at least partially by the interference with microbial membranes due to their hydrophobic character.

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Antibacterial and anti-biofilm activity of cinnamon essential oil and eugenol

Cited by 20 publications

References 20 publications

Decoding antibacterial and antibiofilm properties of cinnamon and cardamom essential oils: a combined molecular docking and experimental study

Decoding antibacterial and antibiofilm properties of cinnamon and cardamom essential oils: a combined molecular docking and experimental study

Anti-biofilm property of essential oils from Cymbopogon sp. against pathogenic bacteria in single-culture and co-culture

Chemical Composition, Antipathogenic and Cytotoxic Activity of the Essential Oil Extracted from Amorpha fruticosa Fruits

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