Anti-biofilm property of essential oils from Cymbopogon sp. against pathogenic bacteria in single-culture and co-culture

Abstract

In this study, we evaluated whether the essential oils (EOs) from Cymbopogon flexuosus and Cymbopogon martinii can prevent production of biofilms either in single or combined culture of Staphylococcus aureus and Pseudomonas aeruginosa. Biofilm formation was assessed by microtiter-plate test with further quantification of viable cells and biofilm biomass. The evaluated EOs at 0.78 % significantly (P 0.05) reduced only the viable cells of S. aureus that inhabited biofilm. However, in single-and co-culture assays, both oils significantly (P 0.05) decreased the amount of biofilm biomass. Biofilm reductions between 52-83% and 60-93% were achieved for the treatments with EOs from C. flexuosus and C. martinii, respectively. Although the biomass reductions of simgle and co-cultivated biofilms were significant, the same was not true for viable cells, except for S. aureus. Considering that the remaining colony forming units can reconstitute the EPS matrix, studies with higher concentrations than those used in this research are suggested in order to obtain greater logarithmic reductions of viable bacterial cells.

Synthesis, computer‐aided ADMET prediction, and molecular docking of novel 3,5,6‐trichloropyridin‐2‐yl derivatives as potential antimicrobial agents

“…Gram-negative bacteria are more likely to resist the antimicrobials than Gram-positive bacteria because they have a different membrane structure. [26,27] The outer membrane of Gramnegative bacteria is the main reason for resistance to a wide range of antibiotics where this phospholipid membrane acts as a barrier and renders the membrane-impermeable to lipophilic components. [28] However, Gram-positive bacteria lack this critical layer, making Gram-negative bacteria more resistant to antibiotics than Gram-positive ones.…”

GC-MS Analysis, Thermal Characterization and Biomedical Applications of Essential Oil from Cymbopogon martinii: In vitro Approach

“…The oil's antibacterial, insect repellent, and pain-relieving characteristics make it important in the pharmaceutical industry [8]. According to Millezi et al [9], the essential oil of Cymbopogon martinii reduces biofilm by 60 to 93 percent (2020).…”