Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria

Abstract

Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria

Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

“…SAE4034 isolate were Gram positive, positive catalase, and positive oxidase. The results of this characterization are in accordance with Ryandini et al (2018).…”

“…The positive control was chloramphenicol which was a broad spectrum antibiotic, while the crude extract still contained a mixture of metabolites, so that the inhibition zone formed by chloramphenicol was wider than the inhibition zone formed by the crude extract.The inhibitory ability of Streptomyces sp. SAE4034 extract against S. aureus was observed for more than 48 hours and still showed consistent inhibitory results it can be said that the extract has stable antibacterial abilities (Ryandini et al, 2018). The MIC test on Streptomyces sp.…”

“…Streptomyces sp. SAE4034 is known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus (Ryandini et al, 2018). However, the group of antibacteria compounds and the mechanism of their inhibition against S. aureus are not yet known.…”

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

“…S. aureus ATCC 6538, E. coli BTCC B 614 and B. subtilis BTCC B 612 with moderate and strong activities. This results linear with Ryandin et al (2018) that reported the broad-spectrum antibacterial activity of crude extracts of actinomycetes isolated from Segara Anakan, Indonesia. They also used agar diffusion method and determined the zone of inhibition from these crude extracts against E. coli, S. aureus, P. aeruginosa and Enterococcus (12.5 mm; 21.5 mm; 10 mm; and 8 mm, respectively).…”

Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria

“…So, novel antibacterial agents are needed from natural biological sources (Valle Jr et al, 2015). Biological antibacterial agents can be obtained from the mushrooms (Prastiyanto et al, 2016), bacteria (Ryandini et al, 2018), bacteriocins (Lestari et al, 2019), and plant (Tillah et al, 2017;Wahyuni et al, 2019). There have been many reports found in the medical literature concerning the significance of traditional medicinal plants as alternatives to antibacterial agents (Akhtar, 2015;Aumeeruddy-elalfi et al, 2015).…”