Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

Abstract

The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. Streptomyces sp. SAE4034 was known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus, but the group of antibacterial compounds and their inhibitory mechanisms were not yet known. The ability of Streptomyces sp. SAE4034 in producing antioxidants is unknown yet. This research aimed to determine the classification of antibacterial compounds produced by Streptomyces sp. SAE4034, to know the ability of Streptomyces sp. SAE4034 in producing antioxidant compounds, and to observe the inhibitory mechanisms of antibacterial compounds produced by Streptomyces sp. SAE4034 against S. aureus using cell leakage method. The results of this research show that Streptomyces sp. SAE4034 produce compounds in the group of alkaloid, terpenoid, flavonoid, polyphenol. Compound with Rf values 0,40 and 0,47 which includes into polyphenol and 0,72 which includes into alkaloid group has the ability to inhibit the growth of S. aureus. Streptomyces sp. SAE4034 has a very strong antioxidant activity that can scavenge DPPH free radical with IC50 value of 32,14 ppm and has an antioxidant enzyme activity that is superoxide dismutase amount of 63,10 U/mL The inhibitory mechanism of Streptomyces sp. SAE4034 extract is through inhibition of protein membrane synthesis by the release of nucleic acid and protein material in S. aureus cells and the highest cell leakage was at 2 MIC doses.

Antioxidant, cytotoxic properties, and chemical constituents of soil Streptomyces spp. isolated from Muna Islands, Southeast Sulawesi, Indonesia

“…In addition, another Streptomyces sp. strain SAE4034 has slightly lower activity with an IC50 DPPH value of 32.14 µg/mL [33]. These results indicated that our potential Streptomyces sp.…”

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

“…QZS11 Soil Ethyl-acetate crude extract [210] Streptomyces sp.MA4 Soil Selenium nanoparticles [211] Streptomyces sp. Sae4034 Rhizosphere soil Alkaloid, terpenoid, flavonoid, and polyphenol [212] Streptomyces levis HFM-2 Human gut 2-Isopropyl-5-methyl-1-heptanol; 1-Octanol, 2-butyl-, 3-Octadecene and 3-Eicosene [213] Streptomyces sp. MUSC Streptomyces colonosanans MUSC 93J T Mangrove soil Ectoine synthesis gene [223] Streptomyces monashensis MUSC 1J T Mangrove soil phenolic compounds, pyrazines and pyrrolopyrazines, Benzoic acid, 9H-Pyrido[3,4-b]indole [224] 6.…”