Antibacterial activity of new peptides from barbel protein hydrolysates and mode of action via a membrane damage mechanism against Listeria monocytogenes

Collagen from Marine Biological Sources and Medical Applications

“…More recently, an AMPs were identified from anchovy cooking wastewater by‐product and showed activity against S. aureus . Anti‐bacterial peptides isolated from barbel ( Barbus callensis ) muscle protein hydrolysates were reported active against several pathogenic bacteria . Additionally, fish secrete several AMPs involved in host defense mechanisms .…”

Fish derived bio-active peptides and their metabolic effects

“…The identified lysozyme cDNA contains 585 nucleotides which encodes a protein of 194 amino acids. Sila et al (2014), evaluated the mode of action of new peptides (Gly-Val-His, Trp-His-Arg, Trp-His-Phe, Pro-Pro-Ser-Ser, Ala-Ala-Ala-Leu, Ala-Ala-Gly-Gly-Val, Ala-Ala-Val-Lys-Met, Ala-Ser-Ser-Ser), previously characterized, from barbel (Barbus callensis) protein hydrolysates against Listeria monocytogenes via a membrane damage mechanism. Prediction of peptide secondary structure indicated that these peptides should have random coil structures and high content of hydrophobic amino acids (Table 2).…”

“…Table 2. Antibacterial activity of synthetic peptides (1mg/1ml) (Sila et al, 2014) Àlvarez et al (2014) have reported on antimicrobial activity of trout hepcidin and stated that peptide showed an alfa-helix conformation in reduced state and the characteristic beta-sheet conformation in the oxidized state. Antimicrobial activity assays showed that the oxidized peptide is more effective than the reduced peptide against Escherichia coli and the important salmon fish pathogen Piscirickettsia salmonis.…”

Novel haemoglobin-derived antimicrobial peptides from chicken (Gallus gallus) blood: purification, structural aspects and biological activity

“…) or pore forming (Sila et al . ), but detailed study of mechanisms of action with a living bacterial cells are rare.…”