Novel haemoglobin-derived antimicrobial peptides from chicken (<i>Gallus gallus</i>) blood: purification, structural aspects and biological activity

Vasilchenko, Alexey S.; Рогожин, Е. А.; Vasilchenko, Anastasia V.; Карташова, О. Л.; Sycheva, M. V.

doi:10.1111/jam.13286

Cited by 9 publications

(6 citation statements)

References 52 publications

(53 reference statements)

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“…A breach in the outer membrane of Gram-negative bacteria was detected using a hydrophobic fluorescent probe. 1- N -phenylnaphthylamine (NPN) is a hydrophobic, neutrally charged substance normally impermeable into the outer membrane, but if the molecules of NPN internalize in phospholipid environments, its fluorescence strongly increases [ 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

et al. 2018

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Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies.

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Section: Resultsmentioning

confidence: 99%

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

et al. 2018

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“…The accumulation of certain probes will reflect the size of the lesions. This approach has been successfully used to determine actions of various AMPs including temporin L , magainin , maculatin , and hemocidines .…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of the indolicidin‐derived novel synthetic peptide In‐58

Vasilchenko

Пашкова

et al. 2017

Journal of Peptide Science

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Natural peptides with antimicrobial activity are extremely diverse, and peptide synthesis technologies make it possible to significantly improve their properties for specific tasks. Here, we investigate the biological properties of the natural peptide indolicidin and the indolicidin-derived novel synthetic peptide In-58. In-58 was generated by replacing all tryptophan residues on phenylalanine in D-configuration; the α-amino group in the main chain also was modified by unsaturated fatty acid. Compared with indolicidin, In-58 is more bactericidal, more resistant to proteinase K, and less toxic to mammalian cells. Using molecular physics approaches, we characterized the action of In-58 on bacterial cells at the cellular level. Also, we have found that studied peptides damage bacterial membranes. Using the Escherichia coli luminescent biosensor strain MG1655 (pcolD'::lux), we investigated the action of indolicidin and In-58 at the subcellular level. At subinhibitory concentrations, indolicidin and In-58 induced an SOS response. Our data suggest that indolicidin damages the DNA, but bacterial membrane perturbation is its principal mode of action. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

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“…It was hypothesized that human blood contains a factor that suppresses toxin-production. Today, it is known that animals’ blood is a source of various peptides including hemocidins, which are the cationic peptide fragments derived from hemoglobin (Mak et al, 2000; Arroume et al, 2008; Vasilchenko et al, 2016). Further studies of the antitoxic effects of hemoglobin showed the ability of globin chains to inhibit all known types of Agr-quorum sensing systems of S. aureus .…”

Section: Effects Of Sub-inhibitory Concentrations Of Antimicrobial Pementioning

confidence: 99%

Sub-inhibitory Effects of Antimicrobial Peptides

Vasilchenko

Рогожин

2019

Front. Microbiol.

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Antimicrobials, and particularly antimicrobial peptides (AMPs), have been thoroughly studied due to their therapeutic potential. The research on their exact mode of action on bacterial cells, especially at under sublethal concentrations, has resulted in a better understanding of the unpredictable nature of bacterial behavior under stress conditions. In this review, we were aiming to gather the wide yet still under-investigated knowledge about various AMPs and their subinhibition effects on cellular and molecular levels. We describe how AMP action is non-linear and unpredictable, also showing that exposure to AMP can lead to antimicrobial resistance via triggering various regulatory systems. Being one of the most known types of antimicrobials, bacteriocins have dual action and can also be utilized by microorganisms as signaling molecules at naturally achievable sub-inhibitory concentrations. The unpredictable nature of AMP action and the pathogenic response triggered by them remains an area of knowledge that requires further investigation.

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Novel haemoglobin-derived antimicrobial peptides from chicken (Gallus gallus) blood: purification, structural aspects and biological activity

Abstract: The obtained results expand the perception of the role of haemoglobin in a living system, describing it as a source of multifunction substances. Additionally, the data presented in this paper may contribute to the development of new, cost-effective, antimicrobial agents.

Cited by 9 publications

References 52 publications

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

Antimicrobial activity of the indolicidin‐derived novel synthetic peptide In‐58

Sub-inhibitory Effects of Antimicrobial Peptides

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