Anti-metastatic effect and mechanisms of Wenshen Zhuanggu Formula in human breast cancer cells

Han, Xin; Wang, Chunli; Xie, Yan; Ma, Jiang; Zhang, Xiao-Hui; Hu, Qianwen; Lin, Ge; Liu, Sheng; Lu, Jin-Gen

doi:10.1016/j.jep.2014.12.036

Cited by 15 publications

(10 citation statements)

References 27 publications

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“…BMSCs are recruited to the stroma of the primary tumor site, where they might facilitate breast cancer cell invasion and distant metastasis [23] . Our previous data have shown that WSZG exerted an inhibitory effect on BMSC-induced MDA-MB-231BO breast cancer cell migration in vitro [10] . However, the anti-metastatic efficacy of WSZG in vivo is unclear.…”

Section: Discussionmentioning

confidence: 93%

“…The results of HPLC analysis were the same as described in our previous in vitro experiment. The content of psoralen, isopsoralen, and osthole in the extract was 15.3, 11.6, and 25.2 mg/g, respectively [10] . …”

Section: Methodsmentioning

confidence: 92%

“…Therefore, we further assessed whether the anti-metastatic effect of WSZG was indirectly dependent on the interaction [10] . The inhibition of the metastatic ability of MDA-MB-231BO cells by WSZG treatment, at least partially, was dependent on the CCL5/CCR5 and IL-17B/IL-17BR axis.…”

Section: Discussionmentioning

confidence: 99%

“…A WSZG extract was prepared according to our previous method [10] . Briefly, Fructus psoraleae, Fructus cnidii, and Aconiti lateralis radix praeparata were mixed at a ratio of 5:5:3.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, BMSCs in the circulating blood are recruited to the site of in situ breast tumor and stimulate the growth of tumors and the frequency of bone metastasis via the interaction between BMSCs and breast cancer cells [9] . In our previous study, we found that WSZG-containing serum exerts an anti-metastatic activity against MDA-MB-231BO breast cancer cells by preventing the interaction between BMSCs and breast cancer cells in vitro [10] . Therefore, in the present study, we further explored the pharmacological activities and potential mechanisms of WSZG in an animal model of breast cancer bone metastases.…”

Section: Introductionmentioning

confidence: 96%

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Wenshen Zhuanggu formula effectively suppresses breast cancer bone metastases in a mouse Xenograft model

Chen

Wang

et al. 2017

Acta Pharmacol Sin

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Wenshen Zhuanggu formula (WSZG) is a traditional Chinese medicine used as an adjuvant for the prevention of bone metastases in breast cancer patients. In this study we investigated the efficacy of WSZG in preventing bone metastases and the potential mechanisms in a mouse xenograft model of breast cancer bone metastases. This model was established by injection of human MDA-MB-231BO-Luc breast cancer cells alone or a mixture of the cancer cells with bone marrow-derived mesenchymal stem cells (BMSCs) into left ventricle of the heart in female nude mice. Then the mice were treated with WSZG (3.25, 6.5 or 13.0 mg·kg -1 ·d -1, ig) for four weeks, whereas zoledronic acid (100 µg/kg per week, ig) was used as a positive control. The occurrence and development of bone metastases were monitored via bioluminescent imaging, and bone lesions were assessed using micro-CT. Intracardiac injection of the mixture of MDA-MB-231BO-Luc breast cancer cells with BMSCs significantly facilitated the bone metastatic capacity of the breast cancer cells, and aggravated bone lesions in the mouse xenograft model of breast cancer bone metastases. Administration of WSZG dose-dependently inhibited the incidence and intensity of bone metastases and protected against bone lesions by suppressing osteoclast formation and tumor cell infiltration. Furthermore, administration of WSZG caused a marked reduction in the expression of CCL5/CCR5 and IL-17B/IL-17BR in bone metastatic tissues. The results demonstrate that WSZG exerts potential therapeutic effects in a mouse xenograft model of breast cancer bone metastases, which are partially mediated by weakening the interaction between BMSCs and breast cancer cells in the tumor microenvironment.

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

“…A WSZG extract was prepared according to our previous method [10] . Briefly, Fructus psoraleae, Fructus cnidii, and Aconiti lateralis radix praeparata were mixed at a ratio of 5:5:3.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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