Anti-elastase, Antioxidant, Total phenolic and Total Flavonoid Content of Macassar Kernels (Rhus javanica L) from Pananjung Pangandaran Nature Tourism Park- Indonesia

Anggraini, Nadia Bunga; Elya, Berna; Iskandarsyah,

doi:10.18311/jnr/2020/24240

Cited by 8 publications

(10 citation statements)

References 18 publications

(21 reference statements)

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“…It was observed that DPPH free radical-scavenging activity of the extract (1.2, 4.63, and 22.86 mg AAE/g n-hexane, acetone, and 80% aqueous methanol respectively) due to the presence of a relatively higher value of total phenol and total flavonoid contents as shown in Previous studies showed the plants of the genus Rhus used in this study, namely Rhus typhina and Rhus javanica reported. The result of Rhus typhina is much better than DPPH radicalscavenging activities reported by on the ethanol fruit extract (3.99, 6.67, and 81.86% at 12.5, 25, and 50 mg/ml, respectively) [4] and the ethanolic extracts of Rhus javanica using DPPH the IC50 values leaves (157.699), steam (561.046), greenish fruit (189.31), and blackish-grey fruit (239.276) using Gallic acid standards [18]. Our results confirm those of Odongo et al(2017) reporting substantial antioxidant activity plant parts from this species.…”

Section: Total Antioxidant Activitymentioning

confidence: 88%

“…The methanolic extract of leaves, stem, and fruit of the Rhus pentaphylla were 71.16, 141.79, 76.88, and Rhus tripartite 64.13, 108.83, and 75.16 respectively in (mg GAE g −1 DW) [17]. On the contrary, the ethanolic extracts of Rhus javanica from leaves (51.91±0.01), steam (28.5 ± 0.03), greenish fruit (53.66 ± 0.01), and blackish-grey fruit (32.74 ± 0.03) using Gallic acid standards [18] is less than the value obtained in this study. This difference could be the difference in the species, environmental (e.g., season, temperature, light, soil), and genetic factors that determine the variation of the antioxidant activity of these compounds.…”

Section: Concentration Vs Absorbancementioning

confidence: 99%

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Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

Belew

W/Hana

Meshesha

et al. 2021

Preprint

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Background: Rhus vulgaris is rich in various classes of polyphenols and flavonoids that act as free radical scavengers and reduce oxidative stress and cure various harmful human diseases. The plant is a traditionally known medicinal plant which is used against a number of diseases including cancer. Methods: The current investigation points towards the investigating quantitative phenolic contents, flavonoid contents and the free radical scavenging activity & antioxidant activity of Rhus vulgaris extract in n-hexane, acetone, and 80% of aqueous methanol. The phenolic contents were estimated by Folin–Ciocalteu procedure and gallic acid as a reference molecule whereas the total flavonoid contents were determined by using aluminum chloride and catechin. In addition, the dilution serial method was used to evaluate the leaves extract of rhus vulgaris, and the DPPH (2,2-diphenyl-1-picrylhydrazyl) method was utilized to assess the above-mentioned extracts against oxidative stress. Results: The result revealed that the ranges of total phenolic content from 5.82 ± 4.6 to 83.15 ± 7.6 mg GAE/g of the dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations were varied from 2.21 ± 7.34to 23.47 ± 4.87 mg CE/g, expressed as catechin equivalents. Antioxidant activity of extracts was expressed as the concentration of DPPH radical’s inhibition ranges from 1.2 ± 0.32 to 22.86 ± 3.71 mg AAE/ g. Conclusion: The 80% aqueous methanolic extract of Rhus vulgaris showed the highest phenolic and flavonoid contents and strong antioxidant potential and it could be used as antibiotics for different curable and incurable diseases.

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Section: Total Antioxidant Activitymentioning

confidence: 88%

Section: Concentration Vs Absorbancementioning

confidence: 99%

Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

Belew

W/Hana

Meshesha

et al. 2021

Preprint

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“…[7]. In the previous research, stem extract of Rhus javanica had the best percent inhibition of elastase activity compared to extract from leaves and fruits (greenish and blackish-grey) [8].…”

Section: Introductionmentioning

confidence: 89%

Antielastase Activity of Macassar Kernels (Rhus javanica) Stem Extract and Skin Elasticity Evaluation of Its Topical Gel Formulation

Anggraini

Elya

Iskandarsyah

2021

Advances in Pharmacological and Pharmaceutical Sciences

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Background. Macassar kernels (Rhus javanica L.) has potential as an antiaging agent as it has antielastase activity, especially its stem extract which has best percent inhibition compared to its leaves and fruit extract. Moreover, the antiaging agent can be commonly used in the form of gel for topical applications. Hence, formulation of HEC-based topical gel from the stem extract of Macassar kernels was conducted. This study aims to determine the antielastase activity of the stem extract of Macassar kernels and evaluate the skin elasticity of its topical gel formulation by conducting dermatological safety and skin antiaging efficacy test. Methods. The stem extract was in vitro tested for antielastase activity using a microplate reader. Then, a formulation of a topical gel containing Rhus javanica stem extract was made. Five stages of quality control, which consisted of an organoleptic test, homogeneity test, pH measurement, viscosity measurement, and physicochemical stability test, were conducted to ensure the quality of topical gel formulation. Last, clinical studies were conducted to evaluate the dermatological safety and antiaging efficacy of gel preparation containing stem extract of Rhus javanica. Results. The stem extract provided antielastase activity (IC50 = 245.68 μg/mL), and its polyphenol was valued at 23.28 ± 1.52 mg GAE/g). The gel containing 10% stem extract had better stability than the gel containing 5% stem extract. The dermatology safety test and efficacy test results indicated that the topical gel containing 10% Rhus javanica stem extract did not cause any skin irritation and significantly improved skin elasticity p < 0.05 . In the treatment group, the moisture parameter was significantly changed on day 14 p < 0.0001 , day 21 p < 0.0001 , and day 29 p < 0.0001 . The elasticity parameter was also changed significantly on day 14 p = 0.0485 , day 21 p = 0.0537 , and day 29 p = 0.0002 . Conclusion. The stem extract of Rhus javanica has potential antielastase activity. The topical gel containing Rhus javanica stem extract also has potential antielastase activity by increasing the skin moisture and enhancing skin elasticity.

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“…The methanolic extracts of leaves, stem, and fruit of the Rhus pentaphylla were 71.16, 141.79, 76.88, and Rhus tripartite 64.13, 108.83, and 75.16, respectively in (mg GAE g − 1 DW) [32]. Another report also revealed an evaluation of total phenolic contents in ethanolic extracts of Rhus javanica from leaves (51.91 0.01), steam (28.5 ± 0.03), greenish fruit (53.66 ± 0.01), and blackish-grey fruit (32.74 ± 0.03) using Gallic acid standards [33] all reports were less than the value obtained in this study. This difference could be the difference in the species, environmental (e.g., season, temperature, light, soil), and genetic factors that determine the variation of the antioxidant activity of these compounds.…”

Section: Total Phenol Total Avonoid Contentsmentioning

confidence: 94%

Evaluation of Total Phenolic, Flavonoid contents, and Antioxidant activity of Rhus vulgaris leaves extracts

Belew

Hana

Meshesha

et al. 2023

Preprint

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Background Rhus vulgaris is rich in various polyphenols and flavonoids that act as free radical scavengers, reduce oxidative stress, and cure various harmful human diseases. Traditionally, the plant is used to treat various diseases including cancer. Methods This study involved extraction using the solvents hexane, acetone, and 80% aqueous methanol, phytochemical screening, and antimicrobial testing. Using the spectrophotometric techniques of Folin-Ciocalteu and aluminum chloride, respectively, the plant's total phenolic and flavonoid contents were determined. The extracts' activity was assessed with the help of the (DPPH) radical scavenging assay. Results The extracts mostly contain alkaloids, phenols, flavonoids, glycosides, tannins, steroids, carbohydrates, and anthraquinones. Compared to the standard, the methanol extracts showed better inhibitory zones. Results revealed that total polyphenols and flavonoid contents were in the range of 5.82 ± 0.6–83.23 ± 0.6 mg GAE/100 g and 2.21 ± 0.34–23.47 ± 0.87 mg CE/100 g, respectively. Leaves extracts of R.vulgaris was found to have higher antioxidant activities ranging from 0.756 ± 0.8 to 131.56 ± 0.6 mg AAE/g sample. Conclusion The R. vulgaris 80% methanolic extract displayed the highest phenolic and flavonoid concentrations as well as a powerful antioxidant capacity. It could be used as an antibiotic for different curable and incurable diseases.

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Anti-elastase, Antioxidant, Total phenolic and Total Flavonoid Content of Macassar Kernels (Rhus javanica L) from Pananjung Pangandaran Nature Tourism Park- Indonesia

Cited by 8 publications

References 18 publications

Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

Antielastase Activity of Macassar Kernels (Rhus javanica) Stem Extract and Skin Elasticity Evaluation of Its Topical Gel Formulation

Evaluation of Total Phenolic, Flavonoid contents, and Antioxidant activity of Rhus vulgaris leaves extracts

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