Background: Rhus vulgaris is rich in various classes of polyphenols and flavonoids that act as free radical scavengers and reduce oxidative stress and cure various harmful human diseases. The plant is a traditionally known medicinal plant which is used against a number of diseases including cancer. Methods: The current investigation points towards the investigating quantitative phenolic contents, flavonoid contents and the free radical scavenging activity & antioxidant activity of Rhus vulgaris extract in n-hexane, acetone, and 80% of aqueous methanol. The phenolic contents were estimated by Folin–Ciocalteu procedure and gallic acid as a reference molecule whereas the total flavonoid contents were determined by using aluminum chloride and catechin. In addition, the dilution serial method was used to evaluate the leaves extract of rhus vulgaris, and the DPPH (2,2-diphenyl-1-picrylhydrazyl) method was utilized to assess the above-mentioned extracts against oxidative stress. Results: The result revealed that the ranges of total phenolic content from 5.82 ± 4.6 to 83.15 ± 7.6 mg GAE/g of the dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations were varied from 2.21 ± 7.34to 23.47 ± 4.87 mg CE/g, expressed as catechin equivalents. Antioxidant activity of extracts was expressed as the concentration of DPPH radical’s inhibition ranges from 1.2 ± 0.32 to 22.86 ± 3.71 mg AAE/ g. Conclusion: The 80% aqueous methanolic extract of Rhus vulgaris showed the highest phenolic and flavonoid contents and strong antioxidant potential and it could be used as antibiotics for different curable and incurable diseases.
Background Rhus vulgaris is rich in various polyphenols and flavonoids that act as free radical scavengers, reduce oxidative stress, and cure various harmful human diseases. Traditionally, the plant is used to treat various diseases including cancer. Methods This study involved extraction using the solvents hexane, acetone, and 80% aqueous methanol, phytochemical screening, and antimicrobial testing. Using the spectrophotometric techniques of Folin-Ciocalteu and aluminum chloride, respectively, the plant's total phenolic and flavonoid contents were determined. The extracts' activity was assessed with the help of the (DPPH) radical scavenging assay. Results The extracts mostly contain alkaloids, phenols, flavonoids, glycosides, tannins, steroids, carbohydrates, and anthraquinones. Compared to the standard, the methanol extracts showed better inhibitory zones. Results revealed that total polyphenols and flavonoid contents were in the range of 5.82 ± 0.6–83.23 ± 0.6 mg GAE/100 g and 2.21 ± 0.34–23.47 ± 0.87 mg CE/100 g, respectively. Leaves extracts of R.vulgaris was found to have higher antioxidant activities ranging from 0.756 ± 0.8 to 131.56 ± 0.6 mg AAE/g sample. Conclusion The R. vulgaris 80% methanolic extract displayed the highest phenolic and flavonoid concentrations as well as a powerful antioxidant capacity. It could be used as an antibiotic for different curable and incurable diseases.
This research work aimed to analyze the total phenolic, flavonoid contents, and antioxidant properties of four selected medicinal plants Amomum subulatum, Lippia adoensis, Coriandrum sativum, and Ruta chalepensis. The Folin-Ciocalteu method to measure total phenolic content, and the aluminum chloride colorimetric method to measure total flavonoid content, the DPPH(1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)), and FRAP radical scavenging assay was used to measure the antioxidant activity. Results showed that Lippia adoensis (50 ± 0.23 mgGAE/g) and (20.5 ± 1.2 mgQE/g) and Ruta chalepensis (45 ± 0.76 mgGAE/g) and (17.3 ± 0.9 mgQE/g) have the highest phenolic and flavonoid content. The highest radical scavenging effect was observed in Lippia adoensis IC50 values 77.3 ± 2.8, 63.2 ± 2.3, and 15.8 ± 1.7 (µg/mL) DPPH, ABTS, and FRAP respectively. There is a positive correlation between total phenolic content, a flavonoid with antioxidant activity. Based on these results of the investigation, it could be concluded that Lippia adoensis is a rich source of phenolic compounds as natural antioxidants of high value.
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