Anti-diabetic activity of methanol/methylene chloride stem bark extracts of Terminalia superba and Canarium schweinfurthii on streptozotocin-induced diabetic rats

Kamtchouing, Pièrre; Kahpui, S.M.; Dzeufiet, Paul Désiré Djomeni; Tedong, Léonard; Asongalem, Emmanuel Acha; Dimo, Théophile

doi:10.1016/j.jep.2005.08.075

Cited by 80 publications

(46 citation statements)

References 8 publications

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“…Similar results were found on eggs and larval worms of H. contortus with acetonic extracts from leaves of three forage legumes (Calliandra calotysus, Gliricidia sepium and leucaena diversifolia) [19]. Furthermore, members of the genus Terminalia are among some of the plants most widely used for medicinal purposes in Africa [19]. This plant also contains phytochemicals like alkaloids, flavonoids, saponins, steroids, phlobatannins, anthraquinones and tannins [20] as well as a rich source of triterpenoids, glycoside derivatives and other aromatic compounds [21].…”

Section: Discussionsupporting

confidence: 81%

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In Vitro Ovicidal and Larvicidal Activities of Stem Bark of Terminalia glaucescens (Combretaceae) against Haemonchus contortus

Lem¹,

Vincent²,

Josué³

et al. 2014

AJPS

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Objectives: To evaluate the in vitro activity of aqueous and methanolic extracts of stem bark of Terminalia glaucescens on the eggs and larval stages of Haemonchus contortus of sheep. Methods: The eggs were exposed for 24 hours in five different concentrations (625, 1250, 2500, 3750 and 5000 µg/ml) of methanol, hot water (decoction) and cold water extracts at room temperature (25˚C). Distilled water, 1.25% DMSO and Albendazole were used as negatives and positive control respectively in the bioassay. Results: A significant effect was obtained with all of the three extracts and differences were observed depending on the parasitic stage. Hot water extract (HWE), Methanol (MET) and Cold water extract (CWE) inhibited embryonic development by 98.1%, 96% and 86.5% respectively at 5000 µg/ml, meanwhile Albendazole had a 77.9% inhibition. For the mean inhibition rate of egg hatching, there was a general increase in the inhibition rate with increase in concentration of the extract from 625 to 5000 µg/ml. Methanol extract had the highest inhibition rate in all concentration from 625 to 5000 µg/ml. Concerning larval mortality, HWE had the highest effect in L1 larval mortality in all concentration above 1250 μg/ml. Distilled water had very little larval mortality on L1 and L2 larvae by 3.3 and 3.3% respectively. Conclusion: This in vitro study brought out the ovicidal and larvicidal properties of stem bark of Terminalia glaucescens.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 61%

In Vitro Ovicidal and Larvicidal Activities of Stem Bark of Terminalia glaucescens (Combretaceae) against Haemonchus contortus

Lem¹,

Vincent²,

Josué³

et al. 2014

AJPS

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“…These A and B compounds were identified as other active principles of plants from the genus Terminalia by the present study. A number of ellagic acid derivatives close to A and B were also isolated from the stem bark of T. superba by some authors (Kamtchouing et al, 2006 ;Tabopda et al, 2008 ;Kuete et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Antifungal activity of Terminalia superba (combretaceae)

Mathieu¹,

Siaka²,

Marcel³

et al. 2015

JEBAS

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KEYWORDS Terminalia superb Anticandidosic Ellagic acid derivative ABSTRACTThe aim of the present study was to optimize the anticandidosic activities of Terminalia superba (TEKAM4) and the identification of major compounds present in the most active chromatographic fraction. The hydroethanolic extract TEKAM4-X0 was prepared by homogenization employing a blender. Two derivatives extracts of TEKAM4-X0 (X1-1 and X1-2) were obtained by a liquid/liquid partition of TEKAM4-X0 in a mixture of hexane and water (v/v). Three chromatographic fractions (F1, F2 and F3) from X1-2 were separated by means of Sephadex-LH20 gel filtration chromatography. All the extracts were incorporated to Sabouraud according to the agar slanted double dilution method. Ketoconazole was used as standards for antifungal assay. The entire fractions were tested on the previously prepared medium culture containing 1000 cells of C. albicans. Antifungal activity was determined by evaluating antifungal parameters values (MFC and IC50). Lastly, the structures of 2 isolated compounds were elucidated by combination of Flash chromatography and spectroscopic methods, including MS, and multiple stage RMN experiments.

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“…Selanjutnya tikus yang mengalami diabetes akan mengalami kerusakan jaringan sel b-langerhans pankreas, sehingga tidak mampu mensintesis insulin, akibatnya kadar glukosa darah menjadi tinggi [17]. Dinyatakan juga bahwa streptozotocin maupun aloksan merupakan senyawa induksi diabetes yang berfungsi merusak sel b-Langerhan pada pankres, sehingga proses sintesis insulin akan terganggu [18] Berdasarkan efek aloksan sebagai induktor diabetes, maka dapat diduga bahwa mekanisme kerja antihiperglikemik ekstrak kulit juwet (Eugenia cumini) adalah berkaitan dengan perbaikan sel-sel b-Langerhan pada pankres yang berfungsi dalam sintesis insulin, sehingga membutuhkan waktu yang cukup lama, yaitu 8 hari. Hal ini sesuai dengan hasil penelitian Saravanan dan Leelavinothan (2008) bahwa pemberian ekstrak air buah juwet menunjukkan adanya peningkatan insulin plasma pada tikus diabet.…”

Section: Hasil Dan Pembahasan Aktivitas Anti-hiperglikemik Ekstrunclassified

Teknik Kromatografi Kolom Vakum Untuk Pemurnian Senyawa Anti-Hiperglikemik Pada Tanaman Juwet (Eugenia Cumini): Tanaman Obat Tradisional Suku Sasak Lombok

Sukib¹,

Kusmiyati²

2011

J. Pijar MIPA

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(1) Progdi. Kimia FMIPA Unram, Jl. Majapahit No. 62 Mataram (0370) Abstrak : Telah dilakukan penelitian tentang penerapan teknik kromatografi kolom vakum untuk pemisahan senyawa anti-hiperglikemik dalam ekstrak metanol kulit kayu juwet Eugenia cumini. Kondisi pemisahan optimum: fasa pendukung/adsorben silikagel G 60 (63 -200 mm) , F = 3,5 cm, l = 30 cm, laju alir 1 ml per menit, dan komposisi eluen Et-OAc:Me-OH (5 : 5). Penentuan aktivitas anti-hipeglikemik dilakukan dengan menggunakan mencit normal dan diabetes induksi aloksan 50 mg/kg bb, yang terbagi menjadi 3 kelompok, kontrol, pelakuan ekstrak/fraksi, dan obat standar metformin. Sampel darah diambil pada hari ke -0, 1, 2, 4, 6, 8, dan 10 sesaat setelah 1 jam perlakuan. Kadar glukosa darah ditentukan dengan metode orto-toluidin. Hasil yang diperoleh menunjukkan bahwa persentase penurunan kadar glukosa darah mencit setelah 8 hari perlakuan adalah: untuk kontrol normal 0%, kontrol diabetes 4%. Untuk perlakuan ekstrak dengan dosis 300 mg/kg terhadap mencit diabetes terjadi penurunan kadar glukosa darah masingmasing: heksana 9%, DCM 18%, Me-OH 38%. Untuk perlakuan fraksi Et-OAc:Me-OH (5 : 5) dengan dosis 50 mg/kg bb terjadi penurunan sebesar 34%, dan untuk obat pembanding metformin (5mg/kg) sebesar 42%. Kesimpulan yang diperoleh dari penelitian ini adalah bahwa ekstrak metanol dari kulit kayu juwet dan fraksi Et-OAc -Me-OH yang diisolasi dengan teknik kromatografi kolom vakum memiliki aktivitas anti-hiperglikemik. Penurunan kadar glukosa darah mencit diabetes memerlukan waktu sampai 8 hari Kata kunci : Kromatogtafi kolom vakum, Juwet, Anti-hiperglikemik, Glukosa Darah VACUUM COLUMN CHROMATOGRAPHIC TECHNIQUE FOR SEPARATION OF ANTI-HYPERGLYCEMIC COMPOUND ON JUWET (EUGENIA CUMINI). TRADITIONAL MEDICINAL PLANT IN SASAK TRIBE LOMBOKAbstract : Vacuum column chromatography technique has been developed for separation anti-hyper-glycemic compound on methanol extract from juwet Eugenia cumini barks. Optimum condition this method are: the adsorbent is silica , F = 3,5 cm, l = 30 cm, elution time 1 ml menit -1 , and solvent system Et-OAc:Me-OH (5 : 5). Normal and alloxan induced diabetic rats were undertaken to evaluated anti-hyperglycemic activity and divided in to three groups, control, extract/fraction, and standar drug metformin. Blood samples were collected one hour after administration on the day 0, 1, 2, 4, 6, 8, and 10. Blood glucose level was determined by the ortho-toluidine method. Treatment with the extract and fraction was isolated from methanol extract resulted in reduction in blood glucose levels after 8 days: for normal control is 0%, diabetic control is 4%. For treatment with the extract at dose 300 mg/kg to diabetic groups decrease in blood glucose is 9% (extract hexane), 18% (extract dichloro-methane), 38% (extract methanol). For treatment with Et-OAc:Me-OH (5 : 5) fraction at dose 50 mg/kg decrease in blood glucose is 34%, and standard drug metformin at dose 5 mg/kg is 42%.The finding of this study indicated that the methanol extract juwet bark and...

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Anti-diabetic activity of methanol/methylene chloride stem bark extracts of Terminalia superba and Canarium schweinfurthii on streptozotocin-induced diabetic rats

Cited by 80 publications

References 8 publications

<i>In Vitro</i> Ovicidal and Larvicidal Activities of Stem Bark of <i>Terminalia glaucescens</i> (Combretaceae) against <i>Haemonchus contortus</i>

<i>In Vitro</i> Ovicidal and Larvicidal Activities of Stem Bark of <i>Terminalia glaucescens</i> (Combretaceae) against <i>Haemonchus contortus</i>

Antifungal activity of Terminalia superba (combretaceae)

Teknik Kromatografi Kolom Vakum Untuk Pemurnian Senyawa Anti-Hiperglikemik Pada Tanaman Juwet (Eugenia Cumini): Tanaman Obat Tradisional Suku Sasak Lombok

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Anti-diabetic activity of methanol/methylene chloride stem bark extracts of Terminalia superba and Canarium schweinfurthii on streptozotocin-induced diabetic rats

Cited by 80 publications

References 8 publications

&lt;i&gt;In Vitro&lt;/i&gt; Ovicidal and Larvicidal Activities of Stem Bark of &lt;i&gt;Terminalia glaucescens&lt;/i&gt; (Combretaceae) against &lt;i&gt;Haemonchus contortus&lt;/i&gt;

&lt;i&gt;In Vitro&lt;/i&gt; Ovicidal and Larvicidal Activities of Stem Bark of &lt;i&gt;Terminalia glaucescens&lt;/i&gt; (Combretaceae) against &lt;i&gt;Haemonchus contortus&lt;/i&gt;

Antifungal activity of Terminalia superba (combretaceae)

Teknik Kromatografi Kolom Vakum Untuk Pemurnian Senyawa Anti-Hiperglikemik Pada Tanaman Juwet (Eugenia Cumini): Tanaman Obat Tradisional Suku Sasak Lombok

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<i>In Vitro</i> Ovicidal and Larvicidal Activities of Stem Bark of <i>Terminalia glaucescens</i> (Combretaceae) against <i>Haemonchus contortus</i>

<i>In Vitro</i> Ovicidal and Larvicidal Activities of Stem Bark of <i>Terminalia glaucescens</i> (Combretaceae) against <i>Haemonchus contortus</i>