Anti-cholesterol triterpenoid acids from Saurauia vulcani Korth. (Actinidiaceae)

Abstract

Two triterpenoid compounds, 3β-hydroxy-Olean-12-en-28-oic acid (1) and 3,19-Dihydroxyurs-12-en-28-oic acid (2) were isolated from the leaves of Saurauia vulcani Korth. (Actinidiaceae). The chemical structures of compounds 1-2 were elucidated with spectroscopic data (UV, IR, 1 H, 13 C, DEPT 135º, HMQC, HMBC, 1 H-1 H COSY NMR) and MS as well as compared with previously reported spectra data. All the compounds were evaluated for their anti-cholesterol activity by the Liebermann-Burchard (LB) colorimetric assay. Compound 1-2 showed a remarkable anti-cholesterol activity. Most importantly, the raised concentration of 1-2 exhibited a dose-dependent manner. The discoveries of anti-cholesterol compounds 1-2 were first reported.

The THE ANTICHOLESTEROL ACTIVITY OF BETULINIC ACID AND STIGMASTEROL ISOLATED FROM THE LEAVES OF SUNGKAI (PARONEMA CANESCENS JACK)

“…Several other triterpenoid compounds have been reported to have anticholesterol properties. The triterpenoid compounds, 3β-hydroxyolean-12-en-28-at acid and 3,19-dihydroxy-urs-12-en-28-at acid found in the Saurauia vulcani Korth, showed anticholesterol activity [15]. Kedaung (Parkia timoriana (DC) Merr) was also found to contain two phytosterol compounds, β-sitosterol and campesterol, which can reduce total blood cholesterol and LDL levels 30-50% by inhibiting cholesterol absorption in the intestinal lumen [25].…”

“…The anticholesterol activity of the compounds was determined using photometry. Cholesterol reacts with the Liebermann-Burchard reagent [15]. Simvastatin was used as a positive control.…”

Quantitative Phytochemical Analysis and Determination of Anti-Cholesterol Activity of Sungkai (Paronema canescens Jack.) Leaf Extracts

“…In a study involving the potential of triterpenoid compounds to lower cholesterol, Bachmid et al, 41 found that an ethanol extract of patikan leaves containing triterpenoid compounds had anti-cholesterol efficacy at doses of 30 mg/kg BW, resulting in a 71 percent reduction in cholesterol levels. Musa et al, 23 reported two triterpenoid compounds from Saurauia vulcani Korth, which demonstrated an exceptional anti-cholesterol activity. The anti-cholesterol properties of this compound are influenced by the presence of hydroxyl groups, double bonds, and carboxylic acid.…”

“…The anti-cholesterol activity of ethanol and methanol extracts, as well as each fraction (n-hexane, ethyl acetate, and methanol), was determined using the photometric method where cholesterol is reacted with the Liebermann-Burchard reagent. 22,23 Simvastatin was used as a positive control. The concentrations of the extracts used were 50, 100, 200, 300, 400, and 500 μg/mL, while the concentrations of the simvastatin used were 10, 20, 30, 40, 50, 75, and 100 μg/mL.…”

A Review on Genus Saurauia: Chemical Compounds and their Biological Activity

“…plant were collected in Silangkitang Village, North Tapanuli, North Sumatera Province, Indonesia. 34 They found two triterpenoid compounds as anti-cholesterol agent, 3β-hydroxy-Olean-12-en-28-oic acid (1) and 3,19-Dihydroxyurs-12-en-28-oic acid (2). The chemical structure of compounds 1 and 2 was shown in Figure 2.…”