Anti-biofilm activity of Thai herbal essential oils against Staphylococcus pseudintermedius causing canine pyoderma

Abstract

The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylococcus pseudintermedius planktonic and biofilm forms. Twenty three bacterial samples were collected from dogs with superficial pyoderma and 9 samples were identified as S. pseudintermedius by polymerase chain reaction-restriction fragment length polymorphism method. The minimum planktonic inhibitory concentration (MPIC), minimum planktonic bactericidal concentration (MPBC) and minimum biofilm eradication concentration (MBEC) of EOs were determined by broth microdilution using a transferable solid phase. Among all, lemongrass EO was the most effective against both planktonic and biofilm forms of S. pseudintermedius with the MPIC, MPBC and MBEC of 0.02, 0.039 and 0.078% v/v, respectively. EOs of betel vine and citronella had the second most inhibitory effect (MPIC and MPBC: 0.039% v/v and MPBC: 0.156% v/v) followed by clove (MPIC and MPBC: 0.078% v/v) and sweet basil (MPIC and MPBC: 0.625% v/v). The EOs of ginger, plai, kaffir lime, turmeric, holy basil and galanga had the lowest inhibitory effect with MPICs and MPBCs ranging from 1.25% v/v to greater than 2.5% v/v. The results of this study indicated that the anti-planktonic and anti-biofilm effects of the tested EOs were concentration dependent, and higher concentrations were required for anti-biofilm activity. Lemongrass EO showed the most potential as a therapeutic antimicrobial agent for both planktonic and biofilm forms of S. pseudintermedius.

Antibacterial efficacy of essential oil spray formulation for post-milking disinfection in dairy cows

“…This study demonstrated that all three essential oils had a good eradicating effect on the common bovine mastitis pathogens, S. aureus, S. agalactiae, and E. coli. The time-kill assays indicated that the antibacterial effects of these essential oils were dependent on both dose and time, which [8,22], in accordance with the results of the present study.…”

Antibacterial activity of cannabis extract (Cannabis sativa L. subsp. indica (Lam.)) against canine skin infection bacterium Staphylococcus pseudintermedius

“…The control bacterium S. pseudintermedius ATCC 49051 was obtained from the American Type Culture Collection (ATCC), Virginia, USA. All strains were cultured in Mueller Hinton broth (MHB) (Becton Dickinson, France) and incubated at 37 °C for 24 h. Inocula were prepared by adjusting overnight cultures to 10 6 -10 7 CFU/ml by measuring the optical density (OD) at 600 nm [10].…”

Lemongrass essential oil enhances antibacterial activity of cephalexin against Staphylococcus pseudintermedius isolated from dogs with superficial pyoderma

“…The bacteria were Gram-positive cocci arranged in grapelike clusters and produced pinpoint colonies that showed beta haemolysis on blood agar. Bacteria that were catalase and coagulase positive, oxidase and hyaluronidase negative, and produced acid from mannitol, sucrose, and trehalose were confirmed to be S. pseudintermedius by PCR-restriction fragment length polymorphism (PCR-RFLP) method as described previously [12]. Briefly, DNA was extracted by BacterialXpress™ nucleic acid extraction kit (Chemicon, Germany) and replicated using primers: pta_f1 (5 -AAA GAC AAA CTT TCA GGT AA-3 ) and pta_r1 (5 -GCA TAA ACA AGC ATT GTA CCG-3 ) (Bio Basic Canada Inc., Canada).…”