Lemongrass essential oil enhances antibacterial activity of cephalexin against Staphylococcus pseudintermedius isolated from dogs with superficial pyoderma

Aiemsaard, Jareerat; Kamollerd, Chuchat; Seubsasana, Supawadee; Thongkham, Eakachai; Vonghataipaisarn, Pairoj

doi:10.2306/scienceasia1513-1874.2021.093

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…Other volatile compounds in essential oil are β-caryophyllene, α-humulen, β-pinene, limonene, farnesol, benzaldehyde, 2-heptanone, and ethyl hexanoate [1]. In other aromatic plants like lemongrass, similar volatile compounds including geraniol, geranial, and citral are the main constituents [26]. In the present study, the hexanic and methanolic extracts obtained from the dried clove buds were analyzed by gas chromatography coupled to mass spectrometry (GC-MS).…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and antifungal and nematicidal activities of the hexanic and methanolic extracts of Syzygium aromaticum

et al. 2023

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In the present study, the fungicidal and nematicidal activities of the hexanic and methanolic extracts of Syzygium aromaticum were evaluated. At a concentration of 100 µg/ml, the hexanic extract inhibited 43.70% of the mycelial growth of Fusarium sp. at 48 h and 52.90% at 72 h; while at the concentration of 200 µg/ml, it inhibited 54.30% and 60% of the mycelial growth of Fusarium sp. at 48 h and at 72 h, respectively. The methanolic extract inhibited 99.87% of the hatching of Haemonchus contortus (H. contortus) eggs at a concentration of 1.25 mg/ml, with respect to the mortality of J2 larvae of it was 71.11% at the concentration of 1.25 mg/ml. Gas Chromatography coupled to mass spectrometry analysis allowed the identification of 17 compounds in total, of which eugenol, acetyl eugenol and caryophyllene were the most abundant in both hexanic and methanolic extracts. Additionally, hexatriacontane, octacosane,11, undecan-3-ol, ledene oxide-(II), and humulene were only present in the methanolic extract, while isoaromadendrene epoxide, 2-naphthalenemethanol, decahydro-α, α, 4atrimethyl-8-methyle, loxapine N -oxide, diepicedrene-1-oxide, aristolochic acid II, and trans-isoeugenol were only found in the hexanic extract.

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Section: Discussionmentioning

confidence: 99%

Chemical composition and antifungal and nematicidal activities of the hexanic and methanolic extracts of Syzygium aromaticum

et al. 2023

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“…In dogs and cats, this disease is generally caused by the bacterium S. pseudintermedius, the yeast M. pachydermatis, and dermatophytes such as M. canis, M. gypseum, and T. mentagrophytes [1]. However, these pathogens are becoming increasingly resistant to the antimicrobial agents routinely used in clinical practice such as oxacillin (methicillin resistance), ampicillin, chloramphenicol, doxycycline, enrofloxacin, gentamicin, and penicillin G for S. pseudintermedius [2][3][4]; and itraconazole, ketoconazole, and miconazole for M. pachydermatis and dermatophytes [5][6][7]. In addition, prolonged use of these drugs can cause allergic reactions, hepatotoxicity, and nephrotoxicity in some animals [8].…”

Section: Introductionmentioning

confidence: 99%

In vitro antimicrobial examination and efficacy of Eryngium foetidum L. extract for skin ointment in animal infectious dermatitis treatment

Punareewattana¹,

Borlace²,

Seubsasana³

et al. 2023

ScienceAsia

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The development of new antimicrobial formulations for the treatment of animal infectious dermatitis is important as current antimicrobial treatments can have undesirable effects. The purpose of this study was to examine the in vitro antimicrobial activity of an Eryngium foetidum L. ethanolic extract against a bacterium, Staphylococcus pseudintermedius; a yeast, Malassezia pachydermatis; and three dermatophytes, Microsporum canis, Microsporum gypseum, and Trichophyton mentagrophytes; all of which are the most common animal skin pathogens causing dermatitis. A viscous semi-solid crude extract was obtained at a yield of 30.6% dry weight. Quantitative analysis of the chemical constituents of the extract by spectrophotometry revealed 3.62 mg GAE/g extract of total phenolic, 1.77 mg CE/g extract of total flavonoid, and 10.11 mg/g extract of total carotenoid contents. HPLC-DAD analysis of the carotenoid content showed that β-carotene and lutein were present at 5.17 and 4.43 mg/g extract, respectively. Antimicrobial activity by broth microdilution test found that the E. foetidum extract had MIC, MBC and MFC values in the range of 6.25 to 25.0 mg/ml; and the time-kill test indicated that this effect was both dose and time dependent. Ointment formulations containing the extract at concentrations of 5, 7.5 and 10% w/w had high microbicidal activity against the tested pathogens and retained their activity and physical properties after repeated freeze-thaw cycles. The results of this study show the potential of E. foetidum extract to be developed as a novel antimicrobial formulation for treatment of infectious dermatitis in pets.

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“…Current treatments for canine superficial pyoderma include systemic and topical antibiotics such as amoxicillin, cephalexin, clindamycin, and mupirocin given over a three to four-week period [3]. The long treatment times and increasing incidence of antibiotic resistance in S. pseudintermedius isolates means that many animals suffer from recurrent infections [4,5]. Moreover, antibiotics not only cause adverse effects such as vomiting, diarrhea, and anorexia but also affect hepatic and renal function [3].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of cannabis extract (Cannabis sativa L. subsp. indica (Lam.)) against canine skin infection bacterium Staphylococcus pseudintermedius

Aiemsaard¹,

Singh²,

Borlace³

et al. 2022

ScienceAsia

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Superficial bacterial skin infections caused by Staphylococcus pseudintermedius are common in dogs. The cannabis plant has shown potential antibacterial effects, but there is limited information about anti-S. pseudintermedius activity. This study investigated the antibacterial activity of the ethanolic extract and the topical formulations of Cannabis sativa L. subsp. indica (Lam.) against 23 S. pseudintermedius isolates obtained from canine pyoderma using broth microdilution and time-kill assays. The cannabis extract's minimum inhibitory concentrations for 50% (MIC 50 ) and 90% (MIC 90 ) of the tested S. pseudintermedius isolates were 6.25 and 12.50 µg/ml, respectively. The MIC for S. pseudintermedius ATCC 49051 was 6.25 µg/ml. The time-kill test revealed that the bactericidal effect of the cannabis extract was time-and not concentration-dependent. Topical formulations containing 0.5, 1, 2, 5, and 10% w/w cannabis extract showed limited in vitro antimicrobial activity in the time-kill test. Only the formulations with cannabis extract of 10% and 5% w/w could reduce the number of viable S. pseudintermedius ATCC 49051 by more than 3-log 10 (99.9%) at 6 and 24 h, respectively. This study demonstrated that the cannabis extract has a good potential to be developed as an antibacterial agent against S. pseudintermedius. However, a clinical trial should be conducted to determine its in vivo therapeutic efficacy.

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Lemongrass essential oil enhances antibacterial activity of cephalexin against Staphylococcus pseudintermedius isolated from dogs with superficial pyoderma

Cited by 6 publications

References 19 publications

Chemical composition and antifungal and nematicidal activities of the hexanic and methanolic extracts of Syzygium aromaticum

Chemical composition and antifungal and nematicidal activities of the hexanic and methanolic extracts of Syzygium aromaticum

In vitro antimicrobial examination and efficacy of Eryngium foetidum L. extract for skin ointment in animal infectious dermatitis treatment

Antibacterial activity of cannabis extract (Cannabis sativa L. subsp. indica (Lam.)) against canine skin infection bacterium Staphylococcus pseudintermedius

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