Anti-anemic potential estimation on mice and characterization of flavonoids using high performance thin layer chromatography in Wrightia tinctoria bark fraction

Bigoniya, Papiya; Shukla, Alok; Singh, Sharda P.; Singh, Chandrashekhar

doi:10.4103/2229-5119.110351

Cited by 14 publications

(12 citation statements)

References 16 publications

(17 reference statements)

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“…Our data showed that VO contained higher amounts of polyphenols than WT, which was more than previously reported values (TPC—35.4 mg/g and TFC—22.8 mg/g; Ebrahimzadeh et al, ). However, TPC of WT is greater than the values reported by Jain, Goyal, and Ramawat (; 19.5 mg/g), in accordance with Bigoniya, Singh, Singh, and Shukla (; 30.46 mg/g), and lower than Ramalakshmi et al (; 55. mg/g). Additionally, TFC and PC followed a trend similar to that of total polyphenols.…”

Section: Resultssupporting

confidence: 68%

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Jamshed

Siddiqi

Gilani

et al. 2019

Phytotherapy Research

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We have reported the antidyslipidemic, antihypertensive, and Ca++ channel blocking activities of Viola odorata (VO) and Wrightia tinctoria (WT). This study extends our understanding of their therapeutic potential by exploring the effects on biomarkers of hepatic and vascular dysfunction together with phytochemical standardization and antioxidant potential. Total phenolic compounds, total flavonoids content, and proanthocyanins of the methanolic extracts were identified using HPLC. Antioxidant capacity was measured using the in vitro assays. Two studies of 6‐week duration were conducted on a high‐fat diet rat model to test the leaves and seed extracts of VO and WT (300 and 600 mg/kg) for their effect on biomarkers for hepatic and vascular dysfunction. The HPLC analysis showed high contents of total phenolic compounds, total flavonoids content, and proanthocyanins along with distinctive phenolic composition. Both extracts exhibited significant antioxidant potential in 1,1‐diphenyl‐2‐picrylhydrazyl, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid), fluorescence recovery after photobleaching, and total antioxidant capacity (TAC) assays, comparable with synthetic standard antioxidants. The in vivo studies indicated a significant reduction in the high‐fat‐diet‐induced rise in serum uric acid, phosphorus, aspartate aminotransferase, alanine aminotransferase, and gamma‐glutamyl transferase. This study indicates the potential of VO and WT to protect from vascular and hepatic damage and an antioxidant effect, thus making these herbs strong candidates for managing cardiometabolic disorders.

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Section: Resultssupporting

confidence: 68%

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Jamshed

Siddiqi

Gilani

et al. 2019

Phytotherapy Research

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“…[20] Methanolic extract of W. tinctoria bark with rich flavonoid content has potential antianemic and hematopoietic effect. [14] Kedar and Chakrabarti have reported that the cholesterol, TG and SGPT levels are increased in case hyperglycemia. Deficiency of insulin caused the increase in the level of these enzymes in liver and serum of diabetic animals.…”

Section: Discussionmentioning

confidence: 99%

“…[12] The methanolic, ethanolic and ethyl acetate extract of W. tinctoria showed rich presence of alkaloid, glycoside, triterpene, tannins and phenolic compounds. [13,14] Until date, no pharmacological evidence for effectiveness of W. tinctoria phenolic and flavonoid content has been reported. Flavonoids contain wide range of biological activity and lot of research has been carried out on their potential role in treating diabetes and other diseases.…”

Section: Introductionmentioning

confidence: 99%

Lack of hypoglycemic activity in total flavonoid fraction of Wrightia tinctoria on alloxan induced hyperglycemia

Shukla¹,

Bigoniya²

2014

J Pharm Negative Results

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Objective: The objective of this study was to investigate the hypoglycemic activity of Wrightia tinctoria (Roxb) (R. Br. Linn., Family-Apocynaceae) total flavonoid (WTTF) fraction on alloxan induced hyperglycemia. Materials and Methods: Total flavonoid fraction from WTTF seed was separated and LD 50 calculated as 1006.93 mg/kg, i.p., following Organization for Economic Co-operation and Development guideline 423. The effect of total flavonoid fraction was observed in three different dose levels 50, 75, and 100 mg/kg. Hypoglycemic activity was carried out using alloxan induced hyperglycemia model. The parameters observed were body weight, blood glucose, total cholesterol, triglycerides (TG), low density lipoprotein, high density lipoprotein, urea and creatinine along with relative organ weight of liver, kidney, and pancreas. Results: Total flavonoid fraction of W. tinctoria did not have any significant effect on reducing glucose level. WTTF had significantly reduced creatinine (P < 0.001), TG (P < 0.05) level and relative weight of liver in diabetic rat at the dose of 100 mg/kg. Conclusion: All the findings indicate absence of antidiabetic activity in W. tinctoria seed but possible presence of liver and kidney protective property.

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“…The results of the RBC tests showed no difference in the number of RBCs between the treatment and control groups, which means the CGEE did not affect erythropoiesis or the osmotic pressure on erythrocyte cells [14]. Some bioactive substances in a plant can induce anemia, which is characterized by low RBC and Hb levels [15,16]. The administration of 5000 mg/kg BW CGEE was not able to induce anemia during treatment.…”

Section: Discussionmentioning

confidence: 99%

The Hematologic Profile in the Acute Toxicity Test of Cogon Grass Roots Ethanol Extract in Wistar Rats

SUMIRAT¹,

PUSPITASARI²,

Anggraeni³

et al. 2020

Int J App Pharm

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Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass roots ethanol extract (CGEE). Methods: Cogon grass roots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data. Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P>0.05). Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters.

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Anti-anemic potential estimation on mice and characterization of flavonoids using high performance thin layer chromatography in Wrightia tinctoria bark fraction

Cited by 14 publications

References 16 publications

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Lack of hypoglycemic activity in total flavonoid fraction of Wrightia tinctoria on alloxan induced hyperglycemia

The Hematologic Profile in the Acute Toxicity Test of Cogon Grass Roots Ethanol Extract in Wistar Rats

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