Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Abstract

We have reported the antidyslipidemic, antihypertensive, and Ca++ channel blocking activities of Viola odorata (VO) and Wrightia tinctoria (WT). This study extends our understanding of their therapeutic potential by exploring the effects on biomarkers of hepatic and vascular dysfunction together with phytochemical standardization and antioxidant potential. Total phenolic compounds, total flavonoids content, and proanthocyanins of the methanolic extracts were identified using HPLC. Antioxidant capacity was measured using the in vitro assays. Two studies of 6‐week duration were conducted on a high‐fat diet rat model to test the leaves and seed extracts of VO and WT (300 and 600 mg/kg) for their effect on biomarkers for hepatic and vascular dysfunction. The HPLC analysis showed high contents of total phenolic compounds, total flavonoids content, and proanthocyanins along with distinctive phenolic composition. Both extracts exhibited significant antioxidant potential in 1,1‐diphenyl‐2‐picrylhydrazyl, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid), fluorescence recovery after photobleaching, and total antioxidant capacity (TAC) assays, comparable with synthetic standard antioxidants. The in vivo studies indicated a significant reduction in the high‐fat‐diet‐induced rise in serum uric acid, phosphorus, aspartate aminotransferase, alanine aminotransferase, and gamma‐glutamyl transferase. This study indicates the potential of VO and WT to protect from vascular and hepatic damage and an antioxidant effect, thus making these herbs strong candidates for managing cardiometabolic disorders.

Khamira Banafsha for the symptomatic treatment of Covid-19 Disease

“…It is used to treat various ailments such as cough, coryza, headache, pleurisy, pneumonia, throat irritation, and complex fever 41; 42; 43; 45 . It possesses various pharmacological properties and has been studied for its antipyretic 58 , anti-inflammatory 59 , antioxidant, vascular protective 60 , neuroprotective 61, antihypertensive, antidyslipidemic 62 , hepatoprotective 63 antitussive, anti-asthmatic 64 and antibacterial activity 65 .…”

Efficacy and safety of add-on Viola odorata L. in the treatment of COVID-19: A randomized double-blind controlled trial

“…The column was monitored at wavelength 280 nm. The mobile phase consisted of 2%(v/v) acetic acid (A) and methanol (B) and the gradient program was set according to the method described by Jamshed H et al with some modifications ( Jamshed et al, 2019 ). The gradient elution was started with 5% B increasing to 30% at 10 mins, then increased from 30% B to 100% B during the next 20 mins at a flow rate of 0.8 ml/min.…”

In-silico molecular docking analysis of some plant derived molecules for anti-inflammatory inhibitory activity

“…Medicinal plants with anti-inflammatory properties were selected from the previous literatures (Aleem et al, 2020;Jeewanthi et al, 2020;Jamshed et al, 2019) and potential compounds identified from the selected plants were used as ligands in this study. The 3D structures of the selected ligands namely 6 urs-12-en-24-oic acid (Figure 2a) and β-Amyrin ( Figure 2b) from Plumbago zeylanica, 10,12-Docasadiyndioic Acid ( Figure 2c) and 1(Ethynyltrimethylsilyl-2)-1-Chloro-2-Ethyl-2-methyl cyclopropane (Figure 2d) from Neolitsea cassia, and Androstane (Figure 2e) from Wrightia tinctoria was collected from Pubchem (http://pubchem.ncbi.nlm.nih.…”