Andean berry (Vaccinium meridionale Swartz) juice, in combination with Aspirin, displayed antiproliferative and pro-apoptotic mechanisms in vitro while exhibiting protective effects against AOM-induced colorectal cancer in vivo

Arango-Varela, Sandra; Luzardo-Ocampo, Iván; Maldonado-Celis, María Elena

doi:10.1016/j.foodres.2022.111244

Cited by 12 publications

(11 citation statements)

References 73 publications

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“…White et al 42 In this study, we chose 1 mg/kg/d and 10 mg/kg/d as the doses of AS for treatment, based on the usual doses of AS utilized in prior studies. [48][49][50][51] According to the findings of this study, both doses of AS…”

Section: Discussionmentioning

confidence: 53%

“…In this study, we chose 1 mg/kg/d and 10 mg/kg/d as the doses of AS for treatment, based on the usual doses of AS utilized in prior studies. 48 , 49 , 50 , 51 According to the findings of this study, both doses of AS alleviated cartilage degeneration in the DMM mouse model, indicating that AS has an anti‐OA effect in the DMM mouse model. According to the calculation methods in Suman et al, 52 1 mg/kg/d and 10 mg/kg/d administered to mice in our study are equivalent to 22.2 mg/d and 222 mg/d in humans, which are within the range of medication doses and are safe to administer to patients in clinical practice.…”

Section: Discussionmentioning

confidence: 55%

“…In this study, we chose 1 mg/kg/d and 10 mg/kg/d as the doses of AS for treatment, based on the usual doses of AS utilized in prior studies 48–51 . According to the findings of this study, both doses of AS alleviated cartilage degeneration in the DMM mouse model, indicating that AS has an anti‐OA effect in the DMM mouse model.…”

Section: Discussionmentioning

confidence: 99%

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Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

et al. 2022

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Bone marrow mesenchymal stem cells (BMMSCs) transplantation methods are promising candidates for osteoarthritis (OA) treatment. However, inflammatory factors (such as TNF-α) that occur at cell transplantation sites are critical factors that impair the effectiveness of the treatment. Previous studies have shown that aspirin (AS) had a regulatory role in stem cell differentiation. However, little is known about the role of AS on the chondrogenesis of BMMSCs. The purpose of this study is to explore the protective role of AS against the negative effects of TNF-α on BMMSC chondrogenesis. In this study, we investigated the effects of AS and TNF-α on BMMSCs chondrogenesis by performing the Alcian Blue staining, safranin O-fast green staining, haematoxylin and eosin staining, and immunohistochemical staining, as well as realtime RT-PCR and western blot assays. Our results demonstrated that TNF-α inhibited chondrogenic differentiation of BMMSCs by disrupting the balance of cartilage metabolism and promoting oxidative stress in BMMSCs, while AS treatment attenuated these effects. Furthermore, a detailed molecular mechanistic analysis indicated that Yes-associated protein (YAP) played a critical regulatory role in this process. In addition, AS treatment mitigated the progression of cartilage degeneration in a mouse destabilization of the medial meniscus (DMM) model. AS alleviated the inhibitory effect of TNF-α on chondrogenesis of BMMSCs by stabilizing YAP, which may provide new therapeutic strategies for OA treatment.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 55%

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Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

et al. 2022

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“…Colorectal cancer (CRC) is currently the third leading cause of cancer death, and global mortality cases are estimated to increase by 60% by the year 2030 [ 11 ]. Although several treatments have been proposed for CRC, most of these procedures involving pharmacological methods display several side effects that could be reduced with the involvement of natural products aiming to either reduce the effect of these treatments or diminish the risk of having CRC [ 12 ]. Genetic and epigenetic alterations are involved in CRC as well as mutations that inactivate the function of the Apc gene and the Ras oncogene, mainly in KRAS.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Phytochemical Composition and Untargeted Metabolomic Analysis of an Extract from Cnidoscolus aconitifolius (Mill.) I. I. Johnst and Porophyllum ruderale (Jacq.) Cass. and Biological Cytotoxic and Antiproliferative Activity In Vitro

Vargas-Madriz

Luzardo-Ocampo

Moreno-Celis

et al. 2023

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Cnidoscolus aconitifolius (CA) and Porophyllum ruderale (PR) are representative edible plants that are a traditional food source in Mexico. This research aimed to analyze the phytochemical composition and untargeted metabolomics analysis of CA and PR and evaluate their antiproliferative effect in vitro. The phytochemical composition (UPLC-DAD-QToF/MS-ESI) identified up to 38 polyphenols and selected organic acids that were clustered by the untargeted metabolomics in functional activities linked to indolizidines, pyridines, and organic acids. Compared with PR, CA displayed a higher reduction in the metabolic activity of human SW480 colon adenocarcinoma cells (LC50: 10.65 mg/mL), and both extracts increased the total apoptotic cells and arrested cell cycle at G0/G1 phase. PR increased mRNA Apc gene expression, whereas both extracts reduced mRNA Kras expression. Rutin/epigallocatechin gallate displayed the highest affinity to APC and K-RAS proteins in silico. Further research is needed to experiment on other cell lines. Results suggested that CA and PR are polyphenol-rich plant sources exhibiting antiproliferative effects in vitro.

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“…Andean blueberry juices are highly appealing in the growing market of functional beverages. It has been suggested by several in vitro studies that these juices exhibit pro‐apoptotic and antiproliferative effects in transformed leukaemic and colorectal cancer cell lines (Arango‐Varela et al ., 2021, 2022). Given their sensory appealing features, their high concentration of phenolic compounds, in particular of anthocyanins, and their bioactivity (Garzón et al ., 2010; Maldonado‐Celis et al ., 2014; Bravo et al ., 2016; Agudelo et al ., 2018; Gallego‐Peláez et al ., 2021), their transformation into more stable products with different functionalities as ingredients have arisen interest (Alzate‐Arbeláez et al ., 2019; Estupiñan‐Amaya et al ., 2020; Garzón et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mixtures of maltodextrin and gum Arabic for the encapsulation of Andean blueberry (Vaccinium meridionale) juice by freeze–drying

Estupiñan‐Amaya

Fuenmayor

López‐Córdoba

2022

Int J of Food Sci Tech

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Summary Andean blueberry fruit is considered a source of bioactive compounds. However, its industrial applications as an ingredient are limited due to its low stability. In this work, Andean blueberry juice powders were obtained via freeze–drying. The effects of maltodextrin (MD) and gum Arabic (GA) as encapsulating agents were evaluated on the morphological, physicochemical, and rheological quality of the products, as well as in terms of the recovery of phenolic compounds. All the freeze–dried powders featured typical macroscopic and microscopic morphology, low moisture content (<5%) and water activity (aw < 0.3), high water solubility (>91%) and good flowability. The encapsulation with MD overall favoured higher water activity, solubility and flowability, and a significant increase of total phenolics recovery in the juice powders, compared with GA or GA:MD mixtures. The results showed that encapsulation by freeze–drying enables the production of Andean blueberry juice powders with suitable technological characteristics and potential as bioactive ingredients.

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Andean berry (Vaccinium meridionale Swartz) juice, in combination with Aspirin, displayed antiproliferative and pro-apoptotic mechanisms in vitro while exhibiting protective effects against AOM-induced colorectal cancer in vivo

Cited by 12 publications

References 73 publications

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

Comparison of Phytochemical Composition and Untargeted Metabolomic Analysis of an Extract from Cnidoscolus aconitifolius (Mill.) I. I. Johnst and Porophyllum ruderale (Jacq.) Cass. and Biological Cytotoxic and Antiproliferative Activity In Vitro

Evaluation of mixtures of maltodextrin and gum Arabic for the encapsulation of Andean blueberry (Vaccinium meridionale) juice by freeze–drying

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