Anatomy of lymph nodes deep cortex in laboratory spices

Abstract

The lymph nodes are organized lymphoid organs in which lymphocytes are located inside reticular stroma. The lymphoid parenchyma of lymph nodes is divided into separated cell zones, the most developed one is parenchyma in which the deep cortex located. The lymph nodes of small mammals, which are often used for laboratory research, The investigated lymph nodes in this research were Somatic (superficial cervical and axillary) and visceral (hepatic and mediastinal) lymph nodes were selected from mice (n = 5), guinea pigs (n = 5), hamsters (n = 3) and rabbits (n = 5). We used classical histological methods (determination of general histological structure of organs), morphometric (determination of the relative area of individual structural and functional zones), immunohistochemical (determination of location of individual cell populations). It was shown that the deep cortex of the parenchyma of the lymph nodes composed by separate structures «deep cortex units», and some of them are combined into complexes. Each unit is represented by a semicircular structure formed by lymphocytes and reticular fibers. The size of units is approximately the same in all studied animals and does not depend on the size of their bodies. The most developed units of the deep cortex were found in the superficial cervical ganglion, and the largest-in rabbits, where their size reached 3 mm. In all studied animals, units of the deep cortex are located at the same level, along the subcapsular sinus. The most developed component tissue of the studied lymph nodes is the lymphoid parenchyma, in laboratory mice it occupies a significantly larger area than in other animal species. The least developed were the central sections of units in the deep coating crust.

Gross Anatomical, Histological, and Cytological Study of the One-humped Camel (Camelus dromedarius) Lymph Nodes in Southeastern Algeria

“…These findings are consistent with the radiological studies by Rahmoun et alb. (2020) and Hussen and Al-Sukruwah (2022) and similar to re-sults observed in lymph nodes of other species, including small laboratory animals (Fares et al, 2019).…”

Morphological features of the lymphatic channel of lymph nodes of in Domestic Bull (Bos taurus)

“…Дослідженням лімфатичних вузлів людини та лабораторних тварин, що мають розвинені субкапсулярні лімфатичні колектори, встановлено, що паренхіма вузлів має пошарово-дискретну структуру та складається з окремих часточок, розташованих в один ряд уздовж відповідного колектора (Belisle & Sainte-Marie, 1981;Gavrilin et al, 2017a;Fares et al, 2019). При цьому основа кожної часточки -це центральна зона одиниці глибокої кори, де відбувається клональна антигензалежна проліферація Т-лімфоцитів, з боку лімфатичного колектора та зовнішніх (бічних) поверхонь одиниць глибокої кори розташовані низькоспеціалізовані зони транзиту лімфоцитів -кіркові плато та паракортикальні тяжі.…”