Gross Anatomical, Histological, and Cytological Study of the One-humped Camel (Camelus dromedarius) Lymph Nodes in Southeastern Algeria

Abstract

Background: The immune system of the dromedary has remained a subject that has not been extensively researched in immunology. Researchers in morphology and immunology have long sought to delve into the structure and function of the dromedary’s immune system to gain a deeper understanding of its mechanisms and potential applications in human and animal health. Objectives: This study aims to elucidate the histological architecture and cellular composition of the lymph nodes in the indigenous dromedary breed of the El Oued region in Algeria and to compare the results with those of prior investigations of lymph node structures in other mammalian species. Methods: Hematoxylin, eosin stain, and Masson’s trichrome stain techniques were used for histological analysis. In contrast, methylene blue, eosin, and May-Grünwald Giemsa staining techniques were used for cytological analysis. The study data were collected and analyzed using qualitative and quantitative methods to identify the histological and cellular features of the lymph nodes. Results: Our study revealed that the lymphatic follicles in the dromedary’s lymph nodes have a higher concentration of lymphocytes within the follicles’ germinal center than other species. The lymph nodes were observed to be divided into conglomerates. The cytological study showed that the major cellular population consisted of lymphocytes, followed by macrophages and reticulocytes according to the localization and the functional zone. Conclusion: The study provided novel insights into the architecture and cellular composition of the lymph nodes of dromedaries, distinct from those of other species. These findings may have implications for the understanding and treatment of immune-related conditions in dromedaries.