Analysis of the effect of okra extract on the diversity of intestinal flora in diabetic rats based on 16S rRNA sequence

Abstract

To study the effect of okra aqueous extract on the intestinal flora of diabetic rats. Taking normal SD rats as CK group, diabetic rats induced by 40 mg/(kg•bw) streptozotocin (STZ) intraperitoneally as M group, normal and diabetic rats gavaged with 500 mg/(kg•bw) okra aqueous extract were CKO and MO groups,respectively. The bacterial flora of the cecum contents of rats was analyzed with 16S rRNA V3-V4 sequencing after 8 weeks of the experiment. Compared with CK, M increased Firmicutes 14.00% and decreased Proteobacteria 19.32% (P <0.01). Compared with M, MO down-regulated Lachnospiraceae 2.64% (such as Blautia, Anaerostipes), up-regulated Peptostreptococcaceae 1.20% in Firmicutes, and down-regulated Desulfovibrioceae 3.78% (such as Desulfovibrio) and up-regulated Burkholderia 5.24% (such as Parasutterella) in Proteobacteria. It is worth noting that MO up-regulated Christensenellaceae (LDA>4), which was negatively correlated with body mass index, inflammation, and metabolic syndrome. The okra aqueous extract reverses the changes of the intestinal flora of STZ-induced diabetic rats, which decreased Firmicutes and increased Proteobacteria by down-regulating Lachnospiraceae and Desulfovibrionaceae, up-regulating Burkholderiaceae, Christensenellaceae. The okra aqueous extract has the potential as a functional food with regulating intestinal microecology balance of diabetes.

Christensenella regulated by Huang-Qi-Ling-Hua-San is a key factor by which to improve type 2 diabetes

“…At the same time, we analyzed the regulation of HQLHS on intestinal microflora, among which the relative abundances of beneficial bacteria such as Bifidobacterium , Romboutsia , Parabacteroides , Parvibacter , and uncultured_bacterium_f_Christensenellaceae were raised. Similar structural changes in gut microflora have been reported, for instance, Christensenellaceae can be up-regulated by Okra extract in diabetic rats ( Wu et al, 2021 ). Despite the emerging findings on the structure of gut microbiota, due to the factors of locality, individuality, and randomness, researchers have not yet reached an agreement on the composition of a healthy gut microbiome ( Proctor, 2019 ).…”

Effects of catalpa seed oil and pomegranate seed oil on body weight and intestinal flora in mice

“…After high-throughput sequencing 16S rRNA sequencing analysis of the composition of the intestinal flora in mice, it was found that at the phylum level, Firmicutes and Bacteroidetes were the two main phyla, which were coordinated with each other. Many studies have found that high-fat-diet-induced obesity could cause changes in the relative abundance ratio of Firmicutes to Bacteroidetes at the phylum level of the gut microbiota (Cheng et al, 2017;Wu et al, 2022). Most studies have shown that the relative abundance ratio of Bacteroidetes to Firmicutes in the intestinal flora of obese individuals was lower than that of nonobese individuals .…”

Metagenomics exploring the effect of recombinant rice based on lotus seed starch-broken rice flour on intestinal flora in rats

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