Analysis of fumonisin B1 in rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection

Analytical methods for determination of mycotoxins: A review

“…These mycotoxins, such as FUMs produced by Fusarium genus, lack a suitable chromophore, and their determination requires derivatisation [1,44]. Examples of derivatising agents are o-phthaldialdehyde and 9-(fluorenylmethyl) chloroformate [114]. Derivatisation can be performed by employing either pre-or post-column [115][116][117][118].…”

Analysis of fumonisin B1 in corn by capillary electrophoresis with fluorescence detection of the FMOC derivative

“…CE analysis with fluorescence detection had already been utilized successfully to analyze the FMOC derivatives of amino acids [ 101. Furthermore, we previously described a sensitive HPLC method for analyzing the fumonisins in corn-based foods by using fluorescence detection of the FMOC derivatives [6]. Therefore, our objective was to determine if CE with fluorescence detection could -.…”

“…These methods involve HPLC with fluorescence detection of the FMOC [6] and fluorescamine [7] derivatives of fumonisins as well as the electrospray mass spectrometry (ESMS) detection of the underivatized fumonisins [81. These HPLC methods are also utilized to perform FBI concentration certification of dosed feeds going to rodents used in the NTP toxicological study of fumonisin B,.…”

Chromatographic separation techniques for determination of mycotoxins in food and feed

“…Nevertheless, in some instances, such as the analysis of PAT in raw apple juice, gradients may be necessary to remove late eluting contaminants from the HPLC column (Sydenham et al, 1995). The determination of FB 1 in rodent feed using 9-fluorenylmethyl-chloroformate is another example of the use of gradient elution to solve difficult separation problems (Holcomb et al, 1993).…”

“…For fumonisin, although a number of fluorescence reagents have been used, including fluorescamine (Ross et al, 1991), 4-fluoro-7-nitrobenzofurazan (NBDF) (Scott and Lawrence, 1992), 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl chloride) (Scott and Lawrence, 1992), 6-aminoquinolyl N-hydroxysuccinimidylcarbamate (Velazquez et al, 1995) and 9-fluorenylmethyl-chloroformate (FMOC) (Holcomb et al, 1993), the most useful derivatives have been those produced using o-phthaldialdehyde (OPA) as a pre-column fluorogenic derivatizing agent . Coupled with strong anion exchange SPE or immunoaffinity column clean up, two methods for the determination of fumonisins in maize using OPA derivatives were validated by collaborative study and achieved AOAC International official status Visconti et al, 2001b).…”