Chromatographic separation techniques for determination of mycotoxins in food and feed

Shephard, G.S.

doi:10.1533/9780857090973.1.71

Cited by 8 publications

(6 citation statements)

References 77 publications

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“…Trends in the application of fast LC techniques, including sample preparation and chromatographic approaches were summarised by Núñez et al (2012). Additional reviews, more general to mycotoxin analysis, included those by Hajslova et al (2011), and Shephard (2011).…”

Section: Introductionmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2011-2012

Shephard

Berthiller

Burdaspal³

et al. 2013

WMJ

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This review highlights developments in mycotoxin analysis and sampling over a period between mid-2011 and mid- 2012. It covers the major mycotoxins aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxin, patulin, trichothecenes, and zearalenone. A section on mycotoxins in botanicals and spices is also included. Methods for mycotoxin determination continue to be developed using a wide range of analytical systems ranging from rapid immunochemical-based methods to the latest advances in mass spectrometry. This review follows the format of previous reviews in this series (i.e. sections on individual mycotoxins), but due to the rapid spread and developments in the field of multimycotoxin methods by liquid chromatography-tandem mass spectrometry, a separate section has been devoted to advances in this area of research.

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Section: Introductionmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2011-2012

Shephard

Berthiller

Burdaspal³

et al. 2013

WMJ

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“…Sample clean-up involves removal of non-mycotoxin "interfering" compounds through liquid extraction, solid-phase extraction (SPE), immunoaffinity-column (IAC), molecular imprinted polymers (MIP) or aptamers (Razzazi-fezeri and Reiter, 2011). Mostly mycotoxin quantitation involves chromatographic technique such as thin-layer chromatography (TLC), gas chromatography (GC) or high-performance liquid chromatography (HPLC) coupled to a photo diode array, UV-Vis or fluorescent detector, or liquid chromatography with mass spectrometry (LC-MS) or tandem mass spectrometry (LC-MS/MS)(Shephard, 2011;Spanjer, 2011). Exception to the above analytical scheme are 'dilute and shoot' methods that bypass sample clean-up but require the use of more sensitive LC-MS/MS equipment(Sulyok et al, 2007;Razzazi-fezeri and Reiter, 2011).…”

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confidence: 99%

Keeping mycotoxins away from the food: Does the existence of regulations have any impact in Africa?

Matumba

Poucke

Ediage

et al. 2015

Critical Reviews in Food Science and Nutrition

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Following the discovery of aflatoxins in the early 1960s, there have been many studies leading to the uncovering of many mycotoxins and the understanding of associated health effects in animals and humans. Consequently, there has been a global increase in the number of countries with mycotoxin regulations in foods. However, many African countries have only regulations for aflatoxins (or a few other mycotoxins) in specific foods, or no regulations at all. This paper critically reviews the challenges thwarting the establishment of mycotoxin regulations and their impacts on human dietary mycotoxin exposure in Africa. Mycotoxin regulatory limits for different countries are compared with mycotoxin tolerable daily intakes established by international food safety bodies taking into account consumption patterns. The agrarian setup, food insecurity, and mycotoxin analytical challenges in African countries are discussed; and more feasible mycotoxin dietary exposure reduction strategies are proposed.

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“…flavus produces mycotoxins called aflatoxins, which are toxic to humans and animals (Mesquita-Rocha et al,2013). Aflatoxins are potent carcinogens which are responsible for many thousands of human deaths mostly in non-industrialized tropical countries (Shephard, 2006). Penicillium species are opportunistic and capable of causing infections such as allergies, asthma and other respiratory problems in immunecompromised patients (Schwab and Strauss, 2004).…”

Section: Discussionmentioning

confidence: 99%

Profile of Fungi Isolated from Domestic Water Used by Street Vendors: The Public Health Implication

Nkenna

2023

clin. med. & helt. res. jour.

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This study aimed at establishing the profile of fungi isolated from domestic water used by street vendors in Owerri metropolis, Imo State from August 2020 to January 2021. Fiftynine water samples collected from 25 street vendors with reference to usage. Data obtained was statistically analyzed with p-value set at 0.05 significance level. The following fungi species were identified in the study; Coccidiodes spp. (5.08%), Penicillium spp. (23.73%), Drechsler spp. (8.47%), Candida spp (5.08%), and Paecilomyces spp. (5.08%). Three Aspergillus species were identified, namely A. flavus (16.95%), A. niger (15.25%) and A. fumigatus (11.84). The prevalence relative to fungi species was statistically significant. The result showed that 28(93.33%) of samples from water used for food preparation was contaminated with fungi, while 26 (89.66%) from drinking water were contaminated, however, the difference was not statistically significant. The result recorded (2.01±1.11 TCFU/100 ml) colony-forming units. The overall number of colonies formed by fungi species was significantly different (p˂0.05). Drechsler spp had the highest count of 4.63±4.41TCFU/100 ml, while the least was observed in Aspergillus niger, 1.07±0.10TCFU/100 ml. The fungi total colony-forming units count was not dependent on the type of water usage, however, samples from water used for food preparation (2.56±2.19TCFU/100 ml) had higher counts than drinking water samples (1.43±0.20 TCFU/100 ml). The result has revealed the presence of some fungi of hazardous inclinations in water used by food vendors in Owerri. There is a need for concerted public enlightenment on the risk of fungi contaminated water and observance of sound safety and hygienic practices.

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Chromatographic separation techniques for determination of mycotoxins in food and feed

Cited by 8 publications

References 77 publications

Developments in mycotoxin analysis: an update for 2011-2012

Developments in mycotoxin analysis: an update for 2011-2012

Keeping mycotoxins away from the food: Does the existence of regulations have any impact in Africa?

Profile of Fungi Isolated from Domestic Water Used by Street Vendors: The Public Health Implication

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