Analysis of fumonisin B₁ in corn by capillary electrophoresis with fluorescence detection of the FMOC derivative

Abstract

Abstract. A new method has been developed for the analysis of fumonisin B, in corn using capillary electrophoresis. Fumonisin B, is the major fumonisin metabolite produced by the fungus Fusarium moniliforme and has been implicated in human and animal diseases. Fumonisin B, was extracted from corn with acetonitrile/water (SO/SO) and cleaned up with a Waters C,, Sep-Pak Vac cartridge. Fumonisin B, was quantitated after elution from the C,, Sep-Pak column using capillary electrophoresis (CE) with a 25 mM sodium borate buffer (pH 9.0) containing 10% acetonitrile and fluorescence detection of the FMOC derivative. The minimum detectable amount in corn was 0.5 ppm. Recovery values in spiked corn averaged over 96% over the range 1-20 ppm. o 1995 John Wiley & Sons, Inc.

Occurrence of fumonisins in foods

“…Detection and quantification are mainly performed by high performance liquid chromatography (HPLC) with fluorimetric (Akiyama, Uraroongroj, Miyahara, Goda, & Toyoda, 1997) or mass spectrometric detection (Musser, 1996). Furthermore, thin-layer (Preis & Vargas, 2000) and gas chromatography (Sydenham et al, 1990b), capillary electrophoresis (Hines, Brueggemann, Holcomb, & Holder, 1995;Holcomb & Thompson, 1996) and enzyme-linked immunosorbent assay (ELISA) (Abouzied, Askegard, Bird, & Miller, 1996;Ono et al, 2001) are also used. A review by Plattner, Weisleder, and Poling (1996) clarifies that extraction with aqueous acetonitrile, clean-up on IAC, followed by derivatization with o-phthaldialdehyde and HPLC on a reverse phase column is a good method of analysis for fumonisins with a detection limit from 0.01 to 0.05 lg/g sample dry weight .…”

Chromatographic separation techniques for determination of mycotoxins in food and feed

“…An immunoaffinity column clean up allowed the determination of FB 1 with a detection limit of 50 µg kg -1 and a migration time of 25 min over a 50 cm length of capillary (Maragos et al, 1996). Holcomb and Thompson (1995) used reversed-phase (C18) SPE for clean up of a maize extract prior to derivatization of the eluate with FMOC for the determination of FB 1 by CE with fluorescence detection at excitation wavelength 210 nm and emission wavelength 305 nm. The detection limit was approximately 500 µg kg -1 .…”

Advanced hyphenated chromatographic-mass spectrometry in mycotoxin determination: Current status and prospects

“…CE combined with MS has been applied in analyses of genetically modified crops and in other food issues including food safety, quality, or food authentication and traceability (Mechref & Novotny, ; Mischak et al, ). After derivation with 9‐fluorenylmethyl chloroformate, FB1 was detected by CE with a fluorescence detector (Holcomb & Thompson, ). ZEA in maize was quantitated using a CE instrument with laser‐induced FD.…”