Analysis of Compounds and Immunostimulatory Properties of Curcuma Mangga Rhizomes on Male Mice

Yuandani, Yuandani; Yuliasmi, Sri; Satria, Denny

doi:10.31788/rjc.2018.1122097

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The amount of quercetin in fermented A. cepa var aggregatum extract was higher after fermentation. Increase in quercetin content in fermented A. cepa var aggregatum was in accordance with previous studies (32,33). This might be due to flavonoid pathway such as glycosylation, deglycosylation, ring termination, methylation, glucuronidation, and sulfoconjugation, which were facilitated by microorganisms (32).…”

Section: Discussionsupporting

confidence: 91%

Anti-hypercholesterolemic activity of standardized fermented Allium cepa L. var aggregatum extract: In vitro and in vivo studies

Rohani

Yuandani

Sitorus

et al. 2022

J Herbmed Pharmacol

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Introduction: Allium cepa extract has been reported to have anti-hypercholesterolemic activity in rats. This study was conducted to investigate the effects of standardized fermented A. cepa L. var aggregatum extract on cholesterol levels and HMG-CoA reductase enzyme. Methods: The fermented A. cepa extract was standardized by the presence of quercetin using a validated high performance liquid chromatography (HPLC) method. The activity of the extract on HMG-CoA reductase was determined using HMG-CoA Assay kits, then measured by Nano spectrophotometry. In vivo study was conducted in hypercholesterolemic rats. The extract was administered orally at doses of 100, 200, and 300 mg/kg body weight (bw) to rats for 21 days and the cholesterol levels were measured every week. Results: All doses of fermented A. cepa extract and its marker compound, quercetin, ameliorated the levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) as compared to those of negative control (P<0.05). Of all the doses, fermented A. cepa extract at the dose of 200 mg/kg bw displayed the highest reduction in LDL-C levels. In addition, the extract at the dose of 200 mg/kg bw showed the strongest enhancement in HDL-C levels. The fermented A. cepa extract and quercetin also inhibited the HMG-CoA reductase enzyme with inhibitory activity of 61.78%. Conclusion: The ethanol extract of fermented A. cepa shows anti-hypercholesterolemic activity. The strong anti-hypercholesterolemic activity of the extract might be due to the high amounts of quercetin, although other constituents may also contribute.

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Section: Discussionsupporting

confidence: 91%

Anti-hypercholesterolemic activity of standardized fermented Allium cepa L. var aggregatum extract: In vitro and in vivo studies

Rohani

Yuandani

Sitorus

et al. 2022

J Herbmed Pharmacol

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“…Safety assessment revealed that it was untoxic in show short-term treatment 15 . The previous study has also shown the immunomodulatory properties of C. mangga 16 . However, the correlation between phytochemical constituents and its immunomodulatory activity was seldom reported.…”

Section: Introductionmentioning

confidence: 83%

CORRELATION BETWEEN THE PHYTOCHEMICAL CONSTITUENTS OF Curcuma mangga AND ITS IMMUNOMODULATORY EFFECT

Yuandani¹,

Yuliasmi²,

Satria³

et al. 2019

RJC

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The present study was conducted to correlate between the phytochemical constituents in the extract of C. mangga rhizomes and its immunomodulatory effect on phagocytosis of mice leukocytes. The phytochemical screening was performed using the standard method, while qualitative analysis of curcumin in C. mangga extract was examined using Thin Layer Chromatography (TLC). The n-hexane, ethyl acetate and ethanol extracts of C. mangga were introduced to carbon clearance method for their immunomodulatory potential. The phytochemical screening on nhexane and ethanol extracts of C. mangga rhizomes revealed the presence of steroids and terpenoids. Meanwhile, glycosides, saponins and flavonoids were detected in ethyl acetate and ethanol extracts. The TLC analysis led to the identification of curcumin in all extracts. All the samples tested demonstrated the immunostimulatory effect on phagocytosis ability of mice leukocytes. Of all the extracts, n-hexane extract displayed the strongest stimulation on phagocytosis effect but there was no significantly different (P>0.05). The results suggest that the immunostimulatory activity of extract on phagocytosis ability was due to the presence of its major constituents although other constituents may also contribute.

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“…It was found to have a variety of active secondary metabolites, such as flavonoids, saponins, glycosides, terpenoids, and steroids ( 11 ). Our previous study has displayed the immunomodulatory activities of C. mangga on phagocytosis, as well as delayed type hypersensitivity reaction and titer antibody by in vivo study in bovine-red blood cells challenged-rats ( 11 12 ). C. mangga has also been evaluated for its toxic effect which revealed that its LD50 was more than 5000 mg/kg BW, indicating that it was nontoxic ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of standardized extract of Curcuma mangga val. on cytokines, antibody and delayed-type hypersensitivity response in Wistar rats

et al. 2021

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Background and purpose: Recently, we have highlighted the immunomodulatory activity of Curcuma mangga Val. on phagocytosis ability. The current study was conducted to determine the immunomodulatory effects of the standardized extract of C. mangga rhizomes by in vitro and in vivo studies. Experimental approach: The C. mangga extract was standardized according to a guideline for herbal preparation. The extract was investigated for its immunomodulatory effects on gene expression of cytokines, cytokines and antibody production as well as delayed-type hypersensitivity (DTH) response. The gene expression of cytokines on lipopolysaccharide-induced-RAW 264.7 cells was analysed by reverse transcription-polymerase chain reaction (RT-PCR) method. The effect of the extract on DTH response was investigated by the paw edema method, meanwhile the effects of the extract on antibody and cytokine production from normal and cyclophosphamide-induced Salmonella typhimurium infected rats were determined using an enzyme-linked immunosorbent assay (ELISA). Findings/Results: The extract of C. mangga demonstrated an inhibitory effect on gene expression of interleukin-1β (IL-1β), tumor necrosis factor-α, and IL-6 as compared to lipopolysaccharide-induced cells. The extract also depicted inhibitory activity on IL-4 production as compared to the negative control. Whereas, the DTH response and production of immunoglobulin G from both groups after treatment with C. mangga extract were higher than those of negative control ( P < 0.05). Conclusion and implications: The results indicated that the C. mangga extract has immunomodulatory effects, emphasizing its potential to be developed as immunotherapeutic agent.

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Analysis of Compounds and Immunostimulatory Properties of Curcuma Mangga Rhizomes on Male Mice

Cited by 5 publications

References 16 publications

Anti-hypercholesterolemic activity of standardized fermented Allium cepa L. var aggregatum extract: In vitro and in vivo studies

Anti-hypercholesterolemic activity of standardized fermented Allium cepa L. var aggregatum extract: In vitro and in vivo studies

CORRELATION BETWEEN THE PHYTOCHEMICAL CONSTITUENTS OF Curcuma mangga AND ITS IMMUNOMODULATORY EFFECT

Immunomodulatory effects of standardized extract of Curcuma mangga val. on cytokines, antibody and delayed-type hypersensitivity response in Wistar rats

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