Analysis of biofilm formation and antibiotic susceptibility pattern of uropathogens in patients admitted in a tertiary care hospital in India

Tayal, Ruchi; Baveja, Sujata; De, Anuradha

doi:10.4103/2278-344x.167648

Cited by 18 publications

(21 citation statements)

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“…Bacteria in biofilm display dramatically increased resistance to antibiotics. 21,22 From the given study performed by us, out of 50 isolates, 32 were biofilm producers (3 strong and 29 moderate) and 18 were none or weak biofilm producers. Among the biofilm producers, cefotaxime was most resistant in 20 of the isolates followed by ceftriaxone in 16 and amoxyclav in 13, whereas amikacin was least resistant in 2 of the isolates.…”

Section: Discussionmentioning

confidence: 99%

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Risal¹,

Shrestha²,

Kunwar³

et al. 2018

Int J Community Med Public Health

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Background: In urinary tract infections, an important role is played by bacterial biofilms which are responsible for persistence infections together with the antimicrobial resistance. Higher resistance can be seen in biofilm forming uropathogens in comparison with free-floating bacteria. So, the present study was performed with a goal to find the prevalence of biofilm formation and also the antimicrobial resistant pattern of uropathogens.Methods: A descriptive method was conducted at Modern Technical College, Sanepa, Lalitpur in samples isolated from UTI suspected patients. The overall duration of this study was approximately 3 months. Total of 50 isolated E. coli was tested for biofilm formation and antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method on Mueller Hinton agar as per CLSI guidelines.Results: From the 50 isolates of E. coli, 32 were biofilm producers (3 strong and 29 moderate) and 18 were weak/non-biofilm producers. Among the biofilm producers, cefotaxime was more resistant in 20 of the isolates followed by ceftriaxone in 16 and amoxyclav in 13, whereas amikacin was least resistant in 2 of the isolates.Conclusions: Among the isolated E. coli, biofilm-forming isolates showed higher antimicrobial resistance as compared to the non-biofilm producer. Thus, uropathogen should be routinely screened for biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Risal¹,

Shrestha²,

Kunwar³

et al. 2018

Int J Community Med Public Health

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“…Similar results were shown by kashef et al (2010) (22) where 85.2% of females had UTI. Also, another study proposed that more than half of patients were females (23) as the anatomical structure of the female genitourinary tract makes them more susceptible to the disease, particularly during pregnancy (10,24) .…”

Section: Discussion:-mentioning

confidence: 99%

Catheter Associated Urinary Tract Infection (Cauti) in Medical Ward, and Icu KFHH During Year 2017.

Zahran¹

2018

IJAR

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Background: Catheter-associated urinary tract infection (CAUTI) is one of the most common hospital acquired infection. Objectives: determining the risk factors, causes and frequency of CAUTI among infected cases with CAUTI in King Fahad Hofuf hospital and to provide local evidence-based recommendations for prevention of CAUTI. Methods: It is a prospective surveillance study of CAUTI. The study was carried out at the Medical ward in King Fahad Hofuf hospital (KFHH) from May to August 2017. The study included all adult patients who are admitted to medical or ICU during the study period with medical conditions rather than UTI and have been catheterized with Foley catheter. The information of the patients were collected from the medical records of the wards and day care unit. Results: the most common causes were sepsis and pneumonia followed by hyperglycemia. Most of the patients showed no symptoms however only 4.5% have fever and there was a positive association between fever and CAUTI as a risk factor for CAUTI. About 29.55 of patients had CAUTI with the majority of them located in ward than ICU. The most common isolated organism among patients with CAUTI are Yeast, candida, E-coli and pseudomonas. The most commonly used antibiotic for CAUTI including cefatrixone, Linezolid, colisitin and fluconazole. CAUTI was significantly associated with female gender. Conclusion: CAUTI was most common among patients located in ward than ICU. The most common risk factors were female gender, fever, previous illness and long duration of illness. Yeast and candida are the most common isolated microorganisms among patients with CAUTI.

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“…In another study, the prevalence of uropathogenic organisms was found to be 88% for E. coli, 4% for Staphylococcus aureus, 2% for P. aeruginosa, 2% for Klebsiella pneumoniae, 3% for Enterococcus, and 1% for Citrobacter koseri. 24 Tayal et al 25 demonstrated that all biofilm-producing isolates of K. pneumoniae were poorly sensitive to the tested antibiotics. The organism showed the highest sensitivity to amikacin at 65%, followed by norfloxacin and nitrofurantoin with 33% sensitivity to each of them.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Effect of Metal Interference in Inhibition of Biofilm Formation by Uropathogenic Escherichia coli Isolated From Hospitalized Patients

Khazaei

Talebi

Hosseni

2019

Int J Basic Sci Med

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Introduction: Bacteria that are able to form biofilms can lead to chronic antibiotic-resistant infections and immunomodulatory effects. Iron and other bivalent metals are essential requirements for biofilm formation by bacteria. Escherichia coli is the most predominant agent causing urinary tract infection (UTI). This study aimed to assess the effects of bivalent metals (iron, zinc, cobalt, and copper) on biofilm formation by E. coli isolated from hospitalized patients suffering from UTI. Methods: A total of 110 E. coli were isolated from 200 UTI patients referred to Farmanieh hospital in Tehran, Iran. E. coli was confirmed by culture specific media, biochemical tests, and polymerase chain reaction (PCR) analysis. To determine the antibiotic resistance, the Kirby-Bauer disk method was used and the biofilm formation was assessed using microtiter plate assay and electron microscopy. Finally, the data were analyzed via paired t test using the SPSS software. Results: Based on our results, out of 110 urine samples containing E. coli, the highest and the lowest resistance were observed to ampicillin (90%) and amikacin (53%), respectively. The biofilm development was intensified in the presence of glucose and iron. The results also indicated that biofilm formation was inhibited by the use of bivalent metal ions including zinc, cobalt, and copper, with the maximum effect obtained for zinc (P < 0.05).Conclusion: Our work led us to conclude that zinc, cobalt, and copper can inhibit biofilm formation by bacterial strains in medicine.

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Analysis of biofilm formation and antibiotic susceptibility pattern of uropathogens in patients admitted in a tertiary care hospital in India

Cited by 18 publications

References 0 publications

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Catheter Associated Urinary Tract Infection (Cauti) in Medical Ward, and Icu KFHH During Year 2017.

Evaluating the Effect of Metal Interference in Inhibition of Biofilm Formation by Uropathogenic Escherichia coli Isolated From Hospitalized Patients

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