Detection of biofilm formation by Escherichia coli with its antibiogram profile

Abstract

Background: In urinary tract infections, an important role is played by bacterial biofilms which are responsible for persistence infections together with the antimicrobial resistance. Higher resistance can be seen in biofilm forming uropathogens in comparison with free-floating bacteria. So, the present study was performed with a goal to find the prevalence of biofilm formation and also the antimicrobial resistant pattern of uropathogens.Methods: A descriptive method was conducted at Modern Technical College, Sanepa, Lalitpur in samples isolated from UTI suspected patients. The overall duration of this study was approximately 3 months. Total of 50 isolated E. coli was tested for biofilm formation and antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method on Mueller Hinton agar as per CLSI guidelines.Results: From the 50 isolates of E. coli, 32 were biofilm producers (3 strong and 29 moderate) and 18 were weak/non-biofilm producers. Among the biofilm producers, cefotaxime was more resistant in 20 of the isolates followed by ceftriaxone in 16 and amoxyclav in 13, whereas amikacin was least resistant in 2 of the isolates.Conclusions: Among the isolated E. coli, biofilm-forming isolates showed higher antimicrobial resistance as compared to the non-biofilm producer. Thus, uropathogen should be routinely screened for biofilm formation.

Detecting of biofilm formation in the clinical isolates of Pseudomonas aeruginosa and Escherichia coli: an evaluation of different screening methods

“…In the MTPA, the number of isolates showing biofilm formation was 38 (38%) (30 strong and 8 moderate) and no weak biofilm producers were detected. A previous study showed from the 50 clinical isolates, 32 were biofilm producers (3 strong and 29 moderate) and 18 were weak/non-biofilm [17]. In another study, of the 50 isolates, 25 each were environmental and clinical.…”

Bi-Laminated Oral Disintegrating Film for Dual Delivery of Pitavastatin Calcium and Lornoxicam: Fabrication, Characterization and Pharmacokinetic Study

“…3 mg of sample and 300 mg of KBr were grounded by means of a mortar and pestle. A small portion of the mixture was compressed at 10 kg/cm in a hydraulic press, resulting in a transparent pellet that was inserted into the sample holder and subjected to scanning in the range of 4000 to 500 cm -1 [5,24].…”

“…4 shows the FT-IR spectra of pure PT, the physical mixture of PT with excipients, pure LN, the physical mixture of PT and LN, and the physical mixture of the optimum PT-ODF with LN (S1 formula). The main peaks of PT are at 1411.06 cm -1 , 1554.46 cm -1 , 2937.06 cm -1 , 3350.30 cm -1 , and 3864.50 cm -1 owing to its functional groups (C=C, C=O, C-H, O-H, and N-H), respectively [5,46]. The major peaks of PT were detected in the physical mixture of PT with excipients, indicating the absence of incompatibility.…”

“…However, discontinuation of statins is commonly detected among patients due to the incidence of numerous side effects, the most common of which is myalgia [1][2][3][4]. Therefore, co-administration of nonsteroidal anti-inflammatory drugs (NSAIDs) with statins can support the treatment of the previously mentioned side effects [5]. NSAIDs are commonly used in the management of muscle pain [6].…”

Biofilms and Antimicrobial Susceptibility Profiles of Escherichia coli Recovered from Wastewater Treatment Plants in Kakamega Municipality, Kenya

“…The biofilm assay was performed following the protocol described by Risal et al (2018) with modifications. Each overnight culture in Tryptone Soy Broth (TSB (HiMedia-India) was diluted to OD 600 = 0.2 (̴ 10 8 CFU/mL), and three 180μL aliquots pippeted into wells of a 96-well microplate.…”