Testicular tissue and sex hormones are sensitive to the anabolic steroids (oxymetholone/OM) due to increased free radical damage and hormonal changes. The Nasturtium officinale L. have various antioxidant compounds. The aim of the present study was to investigate N. officinale effect on OM-induced oxidative injury in mouse testis and sperm parameters. Thirty BALB/c mice were divided into five groups, including control, OM (5 ml/kg) and three N. officinale doses (25, 50 and 100 mg/ kg) + OM. At the end of the study (40 days), serum luteinising hormone (LH), folliclestimulating hormone (FSH), testosterone, nitric oxide (NO) levels, ferric reducing ability of power (FRAP) and testis stereological factors were measured. The sperm parameters were evaluated. Liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS) analysis was yielded a fingerprint of N. officinale phenolic constituents. 100 mg/kg of N. officinale extract significantly reduced the serum level of testosterone and a significant increase in LH and FSH in comparison with the control group. This dose also significantly improved the stereological factors and sperm parameters. 50 and 100 mg/kg of N. officinale extract significantly increased the testis tissue FRAP levels, and 100 doses reduced the serum levels of NO.Fourteen compounds and 34 peaks were identified in the extract with LC-ESI/MS. Nasturtium officinale extract has protective effects against testicular toxicity caused

Breast cancer (BC) is the most common malignancy in women with 18% prevalence and 1 million new cases each year. This cancer showed the highest mortality in the United States and claimed a mortality rate of 89.2 per 100,000 in the world in 2000 (Aurit, Devesa, Soliman, & Schairer, 2019). BC tumors present a heterogeneous phenotype regarding the expressions of different cellular receptors (estrogen receptors [ER], progesterone receptor [PR], and epidermal growth factor receptor 2 [HER2]) in tumor cells. Studies

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Autografting, a major treatment for bone fractures, has potential risks related to the required surgery and disease transmission. Bone morphogenetic proteins (BMPs) are the most common osteogenic factors used for bone-healing applications. However, BMP delivery can have shortcomings such as a short half-life and the high cost of manufacturing the recombinant proteins. Gene delivery methods have demonstrated promising alternative strategies for producing BMPs or other osteogenic factors using engineered cells. These approaches can also enable temporal overexpression and local production of the therapeutic genes in the target tissues. This review addresses recent progress on engineered viral, non-viral, and RNA-mediated gene delivery systems that are being used for bone repair and regeneration. Advances in clustered regularly interspaced short palindromic repeats/Cas9 genome engineering for bone tissue regeneration also is discussed.

The aim of this work is to investigate experimentally and theoretically thermophysical properties (density and viscosity) and derived properties (excess molar volume and viscosity deviation) of pmethylacetophenone + alkanols (C5 to C9) in the range of 293.15 to 323.15 K and a pressure of 0.1 MPa. The properties studied were density, viscosity, excess molar volume, and deviation in viscosity. To model the density of the liquid mixtures, we used the perturbed chain-statistical associating fluid theory equation of state (PC-SAFT EoS) and the volume translated Peng-Robinson-Stryjek-Vera equation of state (VTPRSV EoS). Using a correlation approach an overall deviation of 0.34% and 0.28% was obtained, respectively, while good predictive results were obtained (0.44% using PC-SAFT EoS and 0.46% using VTPRSV EoS). Also, we use two correlations without fitted parameters (Kendall-Munroe correlation and Nguyenhuynh correlation) to model the experimental viscosity and obtained an overall deviation of 5.07% and 3.07%. In addition, the Redlich−Kister correlation (with three fitted parameters) was able to correctly correlate the excess molar volume and the deviation in viscosity for all mixtures. Finally, the results obtained from the work have been discussed in terms of the nature of molecular interactions between the components of the p-methylacetophenone + alkanol mixture.

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Introduction: Raloxifene (Ral) is the oldest SERM (selective oestrogen receptor modulators) for treatment of breast cancer and osteoporosis. Its oestrogen-modulating effects have been shown in breast and uterus. Since there is little available data on direct Ral effect on the human endometrium, the aim of present study was to investigate the Ral effect on the growth and angiogenesis of the human endometrium of healthy and endometriosis subjects in an in vitro three-dimensional (3D) tissue culture model. Material and methods: Endometrial biopsies from healthy ( n = 9) and endometriosis ( n = 7) patients (endometriotic) were taken and were cut into 1 × 1 mm fragments and implanted between two layers of fibrin jell made by fibrinogen solution (3 mg/ml in medium 199+thrombin). Tissue cultures were performed in 24-wel culture plates. Each biopsy was divided into control wells which received M199 supplemented with FBS (5%) and experimental wells which received same media containing one of raloxifene doses (0.1, 1 and 10 μM). Endometrial tissues were photographed at the beginning and the end of the study period (21 days). Tissue growth and angiogenesis were determined by a scoring system. Results: In control (0), 0.1, 1 and 10 μM Ral, the growth score of normal human endometrial tissues were 1.99, 1.72, 1.53 and 1.12 ( p = 0.02) and angiogenesis percent were 29.6%, 31.28%, 33% and 11.5%. The Growth scores of the endometriotic endometrium were 1.92, 1.82, 1.92 and 1.1 ( p = 0.008) and angiogenesis percent were 36.6%, 16.6%, 44% and 12.5% respectively. Conclusion: Raloxifene showed a different dose dependent effect on endometrial and endometriotic tissue.

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