An LC‐MS/MS method for simultaneous determination of vincristine and verapamil in rat plasma after oral administration of a dual agent formulation

Ling, Guixia; Zhang, Peng; Sun, Jin; Zhang, Wenping; Fu, Qiang; Zhang, Tianhong; Deng, Yihui; Zhang, He

doi:10.1002/bmc.1565

Cited by 12 publications

(6 citation statements)

References 24 publications

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“…The presence of doubly charged ions was ensured thanks to the ion trap working in high resolution, but [TAX + 2H] 2+ fragments ion currents were lost during chromatographic runs, probably owing to scarce signal intensities. The collision‐induced fragmentation processes of CDs have been studied by other research groups and reported in many articles (Sottani et al , ; Barbieri et al , ; Ling et al , ; Sun et al , ; Fabrizi et al , ). For this reason, we will not add any more considerations to this subject.…”

Section: Resultsmentioning

confidence: 99%

Dispersive solid‐phase extraction procedure coupled to UPLC‐ESI‐MS/MS analysis for the simultaneous determination of thirteen cytotoxic drugs in human urine

Fabrizi

Fioretti

Rocca

2016

Biomedical Chromatography

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A fast and easy tailored dispersive solid-phase extraction (d-SPE) procedure has been developed for the determination of 13 cytostatic drugs. Combined with a rapid and simultaneous ultra performance liquid chromatography/tandem mass spectrometry method for residue identification and quantification in urine, it has been fully validated and tested to study a realistic situation in working environment. The target compounds were chosen from the most common classes used in hospitals. The d-SPE adsorbent was obtained mixing Oasis HLB® with C18 and applied to a large volume of sample (10 mL). The electrospray ionization-mass spectrometry acquisition was conducted in a mixed period mode: six acquisition windows were in positive ionization and one in negative (for 5-fluorouracil). The lowest limit of quantification was found at 0.04 μg/L urine for methotrexate. The absolute recovery of cytotoxic drugs was assessed at two concentrations levels and ranged from 67.1% (cytarabine) to 102.3% (etoposide) and from 65.3% (cytarabine) to 101.2% (methotrexate) for the lower and higher levels, respectively, with the relative standard deviation always <12%. This method gives the opportunity to analyze drugs in a wide molecular weight range (from 130 to 853 a.m.u.) and in a complex matrix, such as urine, without losing any of the features that a method intended for trace quantification must have. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Dispersive solid‐phase extraction procedure coupled to UPLC‐ESI‐MS/MS analysis for the simultaneous determination of thirteen cytotoxic drugs in human urine

Fabrizi

Fioretti

Rocca

2016

Biomedical Chromatography

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“…[13] performed an analysis of vincristine using only 5 μL of blood plasma, which is less than what was used in the current study, however, the overall volume of whole blood collected was 30 μL, which is three times greater than what was achieved using VAMS devices. The chromatographic run time (6.0 min) lay approximately in the middle in terms of throughput of vincristine, with studies reporting gradients between 2 and 5 min [13, 25, 27]. Conversely, analysis of tariquidar was faster in this study compared to other available methods [30, 33].…”

Section: Discussionmentioning

confidence: 81%

“…Validation parameters achieved in this study are comparable to currently available methods for the quantification of vincristine and tariquidar analytes. Most published studies utilize LC-MS/MS instrumentation for quantification of vincristine [13,[23][24][25][26][27][28][29]and tariquidar [30] over HPLC with ultraviolet detection [31][32][33] given its superior sensitivity. To date, this study has achieved the lowest collected mouse whole blood volume for accurate quantification of both analytes.…”

Section: Discussionmentioning

confidence: 99%

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Rosser

Atkinson

Nath

et al. 2022

J of Separation Science

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A liquid chromatography‐tandem mass spectrometry method was developed and validated for the simultaneous quantification of vincristine and tariquidar in 10 μL of mouse whole blood using volumetric absorptive microsampling devices. Samples were extracted from the devices and quantified against calibrators prepared in a human blood plasma matrix. Separation of vincristine and tariquidar was achieved using a Shimpack XR ODS III C18 stationary phase and H2O and methanol mobile phase solvents containing 0.1% formic acid, running a gradient elution at a flow rate of 0.2 mL/min over 6.0 min. The method was linear up to 1200 ng/mL (R2 > 0.99 for both analytes), with calibrator accuracy within ± 15% of the nominal concentrations and analyte coefficient of variance <15% for both vincristine and tariquidar. Pharmacokinetic assessment of both analytes was successfully applied in mice as both single‐agent therapy and combination therapy over a 24‐h period, and a 2.3‐fold increase in vincristine drug exposure was observed in combination with tariquidar. This study validates the use of this approach for longitudinal analysis of drug exposure in animal studies.

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“…The analysis of vinca alkaloids has been carried out on plant extracts, [398][399][400][401][402][403] pharmaceutical formulations, 316,404 biological samples 358,359,[405][406][407][408][409][410][411][412][413][414][415][416][417][418][419][420]421 and environmental samples. 18,19,134,307,422 Vinca alcaloid extraction from plants was generally performed by ultrasound in acid media followed by LLE.…”

Section: Antitubulin Agentsmentioning

confidence: 99%

Antineoplastic drugs and their analysis: a state of the art review

Guichard

Guillarme

Bonnabry

et al. 2017

Analyst

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The number of patients suffering from cancer is constantly increasing and, consequently, the number of different chemotherapy treatments administered is increasing. Given the high reactivity and toxicity of antineoplastic drugs, analytical methods are required in all pharmaceutical fields, from drug development to their elimination in wastewater; including formulation quality control, environment and human exposure and therapeutic drug monitoring. The aim of this paper is to provide an overview of the analytical methods available for the determination of antineoplastic drugs in different matrices such as pharmaceutical formulations, biological and environmental samples. The applicability and performance of the reported methods will be critically discussed, with focus on the most commonly used antineoplastic drugs. Only conventional compounds and small molecules for targeted therapy will be considered in the present review.

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An LC‐MS/MS method for simultaneous determination of vincristine and verapamil in rat plasma after oral administration of a dual agent formulation

Cited by 12 publications

References 24 publications

Dispersive solid‐phase extraction procedure coupled to UPLC‐ESI‐MS/MS analysis for the simultaneous determination of thirteen cytotoxic drugs in human urine

Dispersive solid‐phase extraction procedure coupled to UPLC‐ESI‐MS/MS analysis for the simultaneous determination of thirteen cytotoxic drugs in human urine

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Antineoplastic drugs and their analysis: a state of the art review

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