An ion‐storage time‐of‐flight mass spectrometer for analysis of electrospray ions

Boyle, James G.; Whitehouse, Craig M.; Fenn, John B.; Cotter, Robert J.

doi:10.1002/rcm.1290050906

Cited by 35 publications

(11 citation statements)

References 15 publications

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“…It relies on ionically charged microdroplets that force the (oppositely charged) protein of interest onto the droplet. Coulombic forces then induce instability on the droplet surface, breaking the original droplet into smaller pieces that contain only the anylate protein of interest (5). …”

Section: Overview Of Proteomic Techniques For the Investigation Of Nementioning

confidence: 99%

Application and Implementation of Selective Tissue Microdissection and Proteomic Profiling in Neurological Disease

Jagannathan

Szerlip

et al. 2009

Neurosurgery

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OBJECTIVE Proteins are the primary components of cells and are vital constituents of any living organism. The proteins that make up an organism (proteome) are constantly changing and are intricately linked to neurological disease processes. The study of proteins, or proteomics, is a relatively new but rapidly expanding field with increasing relevance to neurosurgery. METHODS We present a review of the state-of-the-art proteomic technology and its applications in central nervous system diseases. RESULTS The technique of “selective microdissection” allows an investigator to selectively isolate and study a pathological tissue of interest. By evaluating protein expression in a variety of central nervous system disorders, it is clear that proteins are differentially expressed across disease states, and protein expression changes markedly during disease progression. CONCLUSION Understanding the patterns of protein expression in the nervous system has critical implications for the diagnosis and treatment of neurological disease. As gatekeepers in the diagnosis, evaluation, and treatment of central nervous system diseases, it is important for neurosurgeons to develop an appreciation for proteomic techniques and their utility.

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Section: Overview Of Proteomic Techniques For the Investigation Of Nementioning

confidence: 99%

Application and Implementation of Selective Tissue Microdissection and Proteomic Profiling in Neurological Disease

Jagannathan

Szerlip

et al. 2009

Neurosurgery

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“…capillary columns. These capabilities demonstrate that electrospray LC/MS will play a rapidly increasing role in monitoring our environment Future techniques in LC/MS will address improvements in sensitivity, specificity, analysis time and instrument cost In particular, time-of-flight MS (TOF-MS) (40)(41)(42)(43)(44) and ion trap MS (ITMS) (45)(46)(47)(48)(49) show particular promise, especially when coupled to the rapid and high resolution separation technique of capillary electrophoresis (CE) (50,51). Both TOF and ITMS offer the ability to sample a higher percentage of the ions generated (higher duty cycle), therefore improving signal levels.…”

Section: Conclusion and Future Trendsmentioning

confidence: 98%

Atmospheric Pressure Ionization Liquid Chromatography—Mass Spectrometry for Environmental Analysis

Voyksner

1996

ACS Symposium Series

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“…In an attempt to overcome this limited duty cycle, researchers have developed several methods, including ion storage between batch analyses and fill-time matching in TOFMS [63][64][65][66][67][68]. Importantly, DOFMS provides a unique platform to improve the duty cycle and throughput for velocity-based mass spectrometry.…”

Section: High Duty-cycle Dofmsmentioning

confidence: 99%

Distance-of-Flight Mass Spectrometry: What, Why, and How?

Dennis

Gundlach‐Graham

Ray

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Distance-of-flight mass spectrometry (DOFMS) separates ions of different mass-to-charge (m/z) by the distance they travel in a given time after acceleration. Like time-of-flight mass spectrometry (TOFMS), separation and mass assignment are based on ion velocity. However, DOFMS is not a variant of TOFMS; different methods of ion focusing and detection are used. In DOFMS, ions are driven orthogonally, at the detection time, onto an array of detectors parallel to the flight path. Through the independent detection of each m/z, DOFMS can provide both wider dynamic range and increased throughput for m/z of interest compared with conventional TOFMS. The iso-mass focusing and detection of ions is achieved by constantmomentum acceleration (CMA) and a linear-field ion mirror. Improved energy focus (including turn-around) is achieved in DOFMS, but the initial spatial dispersion of ions remains unchanged upon detection. Therefore, the point-source nature of surface ionization techniques could put them at an advantage for DOFMS. To date, three types of position-sensitive detectors have been used for DOFMS: a microchannel plate with a phosphorescent screen, a focal plane camera, and an IonCCD array; advances in detector technology will likely improve DOFMS figures-of-merit. In addition, the combination of CMA with TOF detection has provided improved resolution and duty factor over a narrow m/z range (compared with conventional, single-pass TOFMS). The unique characteristics of DOFMS can enable the intact collection of large biomolecules, clusters, and organisms. DOFMS might also play a key role in achieving the long-sought goal of simultaneous MS/MS.

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An ion‐storage time‐of‐flight mass spectrometer for analysis of electrospray ions

Cited by 35 publications

References 15 publications

Application and Implementation of Selective Tissue Microdissection and Proteomic Profiling in Neurological Disease

Application and Implementation of Selective Tissue Microdissection and Proteomic Profiling in Neurological Disease

Atmospheric Pressure Ionization Liquid Chromatography—Mass Spectrometry for Environmental Analysis

Distance-of-Flight Mass Spectrometry: What, Why, and How?

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