Atmospheric Pressure Ionization Liquid Chromatography—Mass Spectrometry for Environmental Analysis

The health-promoting effects of whole-grain consumption have been attributed in part to their unique phytochemical contents and profiles. Wheat is an important component of the human diet; however, little is known about the phytochemical profiles of different wheat varieties, especially of old wheats. The objective of this study was to investigate the distribution of lignans, a class of phytochemicals with proved health benefit effects, of four modern and six old Italian soft wheat (Triticum aestivum L.) cultivars. In this work, we describe the first analytical method involving CE coupled to MS (CE-MS) used to identify and quantify lignan compounds in grains of different cultivars of wheat. Total lignan content determined by CE-ESI-MS was 2.60+/-0.21 and 5.00+/-1.30 microg/g dry seed weight for modern and old cultivars, respectively. Secoisolariciresinol and pinoresinol were detected in all ten investigated soft wheat cultivars, whereas arctigenin, hinokinin, and syringaresinol were exclusively detected in old genotypes. Significant differences between modern and old cultivars were also observed for the number of glycosidic forms. Results highlighted the high content and unique composition in lignans of old cultivars suggesting their uses into a wide range of regular and specialty food products naturally enriched with health-promoting compounds.

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“…Generally, small amounts of volatile TEA or ammonium hydroxide can be used for ESI-negative detection [25].…”

Section: Setting Up Of Ce-esi-ms Methodsmentioning

confidence: 99%

Lignan profile in seeds of modern and old Italian soft wheat (Triticum aestivum L.) cultivars as revealed by CE‐MS analyses

et al. 2007

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“…Bare silica columns operated with low aqueous-high organic mobile phases are viable means of analyzing polar compounds in biological fluid. Mobile phases containing highly organic solutions would lead to favorable spraying conditions at the LC-MS interface necessary for adequate sensitivity (Voyksner, 1997). Even though TFA was reported to suppress electrospray signals due to its ion-pairing in the gas phase with the analyte ions (Kuhlmann et al, 1995), the gain of sensitivity by using higher organic content was so large that the overall sensitivity was still enhanced.…”

Section: The [M+h]mentioning

confidence: 99%

Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometric method for the analysis of paroxetine in human plasma

Weng

Eerkes

2003

Biomedical Chromatography

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A sensitive, simple, fast and rugged hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for the determination of paroxetine was developed and validated over curve range 0.050-50 ng/mL using only 0.4 mL plasma. This is the first published LC-MS/MS method and the low limit of quantitation of this method is 10-fold lower than previously published methods. A simple liquid-liquid extraction method using methyl-tert butyl ether (MTBE) as the extraction solvent was used to extract paroxetine and the internal standard (IS) fentanyl-d(5) from plasma. The extract was evaporated to dryness, reconstituted and injected onto a silica column using a low aqueous-high organic mobile phase. The chromatographic run time was 2.0 min per injection, with retention times of 1.1 and 1.2 min for paroxetine and IS, respectively. The detection was by monitoring paroxetine at m/z 330 --> 192 and IS at m/z 342 --> 188, respectively. The inter-day precision and accuracy of the quality control (QC) samples were <5.0% relative standard deviation (RSD) and <2.9% relative error (RE). This method can be used for supporting therapeutical drug monitoring and pharmacokinetic or drug-drug interaction studies.

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“…Direct-EI and Cap-EI interfaces were tested with several compounds of environmental, forensic, pharmaceutical, and biological interest under very different analytical conditions (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Pesticides, hormones, coumarins, explosives, and nitro-PAHs were determined at trace-level concentrations in various matrixes, demonstrating good versatility of the approach.…”

Section: Performance and Applicationsmentioning

confidence: 99%

“…In addition, if the anion and cation pair too strongly with the analyte, then the analyte ions may be prevented from carrying the excess charge on the droplet surface, and a very low ESI response may result. For these reasons, when ESI is interfaced with HPLC or CE, volatile buffers composed of weak acids and bases must replace nonvolatile modifiers (29).…”

Section: Performance and Applicationsmentioning

confidence: 99%

Peer Reviewed: Electron Ionization for LC/MS

Cappiello¹,

Famiglini²,

Palma³

2003

Anal. Chem.

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Atmospheric Pressure Ionization Liquid Chromatography—Mass Spectrometry for Environmental Analysis

Cited by 16 publications

References 43 publications

Lignan profile in seeds of modern and old Italian soft wheat (Triticum aestivum L.) cultivars as revealed by CE‐MS analyses

Lignan profile in seeds of modern and old Italian soft wheat (Triticum aestivum L.) cultivars as revealed by CE‐MS analyses

Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometric method for the analysis of paroxetine in human plasma

Peer Reviewed: Electron Ionization for LC/MS

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