An insight into the use of dimethylphenyl carbamate cyclofructan 7 chiral stationary phase in supercritical fluid chromatography: The basic comparison with HPLC

The interest of pharmaceutical companies for complementary high-performance chromatographic tools to assess a product's purity or enhance this purity is on the rise. The high-throughput capability and economic benefits of supercritical fluid chromatography, but also the "green" aspect of CO2 as the principal solvent, render supercritical fluid chromatography very attractive for a wide range of pharmaceutical applications. The recent reintroduction of new robust instruments dedicated to supercritical fluid chromatography and the progress in stationary phase technology have also greatly benefited supercritical fluid chromatography. Additionally, it was shown several times that supercritical fluid chromatography could be orthogonal to reversed-phase high-performance liquid chromatography and could efficiently compete with it. Supercritical fluid chromatography is an adequate tool for small molecules of pharmaceutical interest: synthetic intermediates, active pharmaceutical ingredients, impurities, or degradation products. In this review, we first discuss about general chromatographic conditions for supercritical fluid chromatography analysis to better suit compounds of pharmaceutical interest. We also discuss about the use of achiral and chiral supercritical fluid chromatography for analytical purposes and the recent applications in these areas. The use of preparative supercritical fluid chromatography by pharmaceutical companies is also covered.

Section: Chromatographic Conditions For Sfc Analysis Of Drug Moleculesmentioning

confidence: 99%

Use and practice of achiral and chiral supercritical fluid chromatography in pharmaceutical analysis and purification

Lemasson

Bertin

West

2016

“…Compared with native CFs, which have rather limited chiral recognition ability, the chiral stationary phases of aliphatic and aromatic functionalized CF6 with unique and very different selectivities can separate a very broad range of racemic compounds. Since then, a series of new chiral stationary phases using different CF derivatives as chiral selectors have been further developed in HPLC , GC , and CE by Armstrong et al.…”

Section: Cfs Derivatives As Chiral Stationary Phases For Gcmentioning

confidence: 99%

Recent progress of chiral stationary phases for separation of enantiomers in gas chromatography

Xie

Yuan

2016

104

Chromatography techniques based on chiral stationary phases are widely used for the separation of enantiomers. In particular, gas chromatography has developed rapidly in recent years due to its merits such as fast analysis speed, lower consumption of stationary phases and analytes, higher column efficiency, making it a better choice for chiral separation in diverse industries. This article summarizes recent progress of novel chiral stationary phases based on cyclofructan derivatives and chiral porous materials including chiral metal-organic frameworks, chiral porous organic frameworks, chiral inorganic mesoporous materials, and chiral porous organic cages in gas chromatography, covering original research papers published since 2010. The chiral recognition properties and mechanisms of separation toward enantiomers are also introduced.

“…LFER model has been used many times for the characterization of retention mechanisms in achiral and chiral separation environments of HPLC and SFC either separately or in combination with another model. As an example of its typical application and output a characterization of novel metallacarborane‐based HPLC stationary phases is shown in Fig.…”

Section: Characterization Of Hplc and Sfc Separation Systemsmentioning

confidence: 99%

Chromatographic methods enabling the characterization of stationary phases and retention prediction in high‐performance liquid chromatography and supercritical fluid chromatography

Sýkora

Vozka

Tesařová

2015

In the scope of the present review, the current status of high-performance liquid chromatography/ultra-high performance liquid chromatography and supercritical fluid chromatography is briefly provided. These techniques and their retention mechanisms are compared. Various alternative approaches utilized for the determination and description of the retention processes in these two systems are mapped. Two frequently used concepts, linear-free energy relationships, and hydrophobic subtraction models, used for the characterization of the retention interactions, are discussed. Principles and selected applications of the both methods are also covered. Then the models applied for the prediction of retention behavior of solutes on stationary phases are outlined. The procedures utilized for the sorbent/column classification are also covered. Simple chromatographic tests frequently used for the basic characterization and mutual comparison of stationary phases are summarized and briefly commented on. The importance of a statistical evaluation of complex retention data obtained from the chromatographic measurements is outlined. Finally, computer simulations aiming at the facilitation of the quest to optimize separation conditions for a given mixture of analytes are touched upon.