An in vitro study on the influence of viscosity and frequency of application of fluoride/tin solutions on the progression of erosion of bovine enamel

Sakae, Letícia Oba; Bezerra, Sávio José Cardoso; João-Souza, Samira Helena; Borges, Alessandra Bühler; Aoki, Idalina Vieira; Aranha, Ana Cecília Corrêa; Scaramucci, Taís

doi:10.1016/j.archoralbio.2018.01.017

Cited by 31 publications

(22 citation statements)

References 26 publications

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“…One important aspect when studying filmforming polymers is the fact that many of them are thickening agents, which means that they could increase the viscosity of solutions [Ávila et al, 2017]. It is known that the viscosity of a solution can affect its anti-erosive potential [Sakae et al, 2018], since we envision their use as additives to mouth rinses, it is necessary to verify how they would affect their final viscosity. Zeta potential is the electrostatic potential of the hydroxyapatite surface that extends to the liquid phase, when the ions on the surface are in equilibrium with those of the solution [Young et al, 1997].…”

Section: Introductionmentioning

confidence: 99%

Anti-Erosive Effect of Solutions Containing Sodium Fluoride, Stannous Chloride, and Selected Film-Forming Polymers

et al. 2018

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The aim of this study was to evaluate the anti-erosive effect of solutions containing sodium fluoride (F: 225 ppm F^–), stannous chloride (Sn: 800 ppm Sn2+), and some film-forming polymers (Gantrez: Poly [methylvinylether-alt-maleic anhydride]; PGA: propylene glycol alginate; Plasdone: poly[vinylpyrrolidone]; and CMC: carboxymethylcellulose). Solutions were tested in an erosion-remineralization cycling model, using enamel and dentin specimens (n = 10, for each substrate). Distilled water was the negative control. Cycling consisted of 120 min immersion in human saliva, 5 min in 0.3% citric acid solution, and 120 min of exposure to human saliva, 4×/day, for 5 days. Treatment with solutions (pH = 4.5) was carried out 2×/day, for 2 min. Surface loss (SL) was evaluated with optical profilometry. Zeta potential of hydroxyapatite crystals was determined after treatment with the solutions. Data were statistically analyzed (α = 0.05). For enamel, all polymers showed significantly lower SL (in µm) than the control (11.09 ± 0.94), except PGA (10.15 ± 1.25). PGA significantly improved the protective effect of F (4.24 ± 0.97 vs. 5.64 ± 1.60, respectively). None of the polymers increased the protection of F+Sn (5.13 ± 0.78). For dentin, only Gantrez (11.40 ± 0.97) significantly reduced SL when compared with the negative control (12.76 ± 0.75). No polymer was able to enhance the effect of F (6.28 ± 1.90) or F+Sn (7.21 ± 1.13). All fluoridated solutions demonstrated significantly lower SL values than the control for both substrates. Treatment of hydroxyapatite nanoparticles with all solutions resulted in more negative zeta potentials than those of the control, except Plasdone, PGA, and F+Sn+PGA, the latter two presenting the opposite effect. In conclusion, Gantrez, Plasdone, and CMC exhibited an anti-erosive effect on enamel. PGA increased the protection of F. For dentin, only Gantrez reduced erosion.

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Section: Introductionmentioning

confidence: 99%

Anti-Erosive Effect of Solutions Containing Sodium Fluoride, Stannous Chloride, and Selected Film-Forming Polymers

et al. 2018

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“…Previous studies have shown that increasing the viscosity of fluoride‐containing solutions (NaF and/or NaF/AmF/SnCl 2 ) improves their enamel erosion reducing effects in a 5‐d erosion experiment involving four daily applications of 0.3% citric acid for 5 min (19) and in a 10‐d experiment involving six daily applications of 0.5% citric acid for 2 min (18). However, there is no study testing the effect of the addition of chitosan to the fluoride solutions with respect to dentin erosion in these experimental circumstances.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the increased viscosity in fluoridated (NaF and amine fluoride — AmF) solutions containing stannous ions reduced enamel erosion in a 5 d (19) and 10 d experiment (18). However, there is only one study on the effect of chitosan solution and AmF/NaF/SnCl 2 (not associated with chitosan) on the prevention of dentin erosion (20).…”

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confidence: 99%

Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro

Souza

Machado

Vecchia

et al. 2020

European J Oral Sciences

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The aim of this study was to assess the protective effect of experimental solutions containing chitosan at different viscosities with or without fluoride (TiF 4 /NaF) on dentin loss in vitro. Bovine dentin samples (n = 15) were prepared and allocated to one of the following treatments: (i) 0.5% chitosan (500 mPas); (ii) 0.5% chitosan (2,000 mPas); (iii) 0.042% NaF and 0.049% TiF 4 ; (iv) as (iii) with addition of 0.5% chitosan (500 mPas); (v) as (iii) with addition of 0.5% chitosan (2,000 mPas); (vi) commercial solution with SnCl 2 /AmF/NaF (positive control); or (vii) deionized water (negative control). The samples were submitted to pH cycling for 7 d (0.1% citric acid, 4 9 90 s d À1). The treatment was applied once a day for 30 s. The dentin loss was quantified using a contact profilometer. Three samples per group were evaluated using scanning electron microscopy. The dentin loss (lm) was submitted to ANOVA and Tukey's test for differences between treatments. Among the treatments tested, only chitosan 500 mPas was able to statistically significantly reduce the dentin loss compared to the negative control, being similar to the positive control. TiF 4 /NaF, whether with or without chitosan, had no protective effect. Chitosan 500 mPas and SnCl 2 /AmF/NaF solutions have comparable protective effect against dentin erosion in vitro.

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“…These deposits behave as a physical barrier that reduces contact of the acids with the underlying enamel and act as a mineral reservoir which is first attacked by the erosive challenge [Magalhães et al, 2011]. Therefore, to standardize the pH conditions and favor the deposition of these precipitates, the pH of the prepared fluoride solutions was adjusted to 4.5 [Sakae et al, 2018;Bezerra et al, 2019]. Additionally, this acidic pH of the solutions allowed the maintenance of a low viscosity in the solutions associated with Carbopol.…”

Section: Discussionmentioning

confidence: 99%

“…In order to avoid a potential confounding factor [Sakae et al, 2018], the dynamic viscosity of the solutions was determined using a vibroviscosimeter (SV-10, A&D, Toshima-ku, Tokyo, Japan). The results were obtained in duplicate in a temperature-controlled room (25 ° C ± 1 ° C).…”

Section: Preparation Of the Solutions And Measurement Of Their Viscositymentioning

confidence: 99%

Enhancing the Anti-Erosive Properties of Fluoride and Stannous with the Polymer Carbopol

et al. 2020

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This in vitro study investigated whether Carbopol 980 polymer could potentiate the anti-erosive effect of solutions containing sodium fluoride (F) and sodium fluoride associated with stannous chloride (FS). The dissolution of hydroxyapatite treated with the experimental solutions (F [500 ppm F–], F + Carbopol [0.1%], FS [500 ppm F– + 800 ppm Sn2+], FS + Carbopol) was evaluated. Deionized water was the negative control, and a commercial mouth rinse (AmF/NaF/SnCl2; 500 ppm F + 800 ppm Sn2+; Elmex® Erosion Protection) was the positive control. The solutions were also evaluated in an erosion-rehardening protocol, with two treatments per day, using bovine enamel specimens (n = 15) and human saliva. The acid challenge was performed using 0.3% citric acid (pH 2.6) for 2 min. Microhardness was measured at different times: baseline, after the first erosive challenge, after treatment, and after the second erosive challenge. Based on microhardness values, the demineralization, rehardening, and protective potentials were calculated. The alkali-soluble fluoride on enamel surfaces was also measured. Data were analyzed using ANOVA and Tukey tests (α = 0.05). Groups treated with FS + Carbopol showed the lowest hydroxyapatite dissolution and the highest rehardening and protective potentials. The measurement of alkali-soluble fluoride on enamel surfaces was also higher in the FS + Carbopol group. Carbopol was able to significantly increase the protective effect of the fluoridated solutions in addition to optimizing the adsorption of fluoride on the enamel surface.

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An in vitro study on the influence of viscosity and frequency of application of fluoride/tin solutions on the progression of erosion of bovine enamel

Abstract: Under the conditions of this short-term in vitro experiment, it could be concluded that increasing the viscosity of the oral rinse solutions reduced enamel loss by erosion; however, this effect was small and only observed when the solutions were applied once a day.

Cited by 31 publications

References 26 publications

Anti-Erosive Effect of Solutions Containing Sodium Fluoride, Stannous Chloride, and Selected Film-Forming Polymers

Anti-Erosive Effect of Solutions Containing Sodium Fluoride, Stannous Chloride, and Selected Film-Forming Polymers

Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro

Enhancing the Anti-Erosive Properties of Fluoride and Stannous with the Polymer Carbopol

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