Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro

Abstract

The aim of this study was to assess the protective effect of experimental solutions containing chitosan at different viscosities with or without fluoride (TiF 4 /NaF) on dentin loss in vitro. Bovine dentin samples (n = 15) were prepared and allocated to one of the following treatments: (i) 0.5% chitosan (500 mPas); (ii) 0.5% chitosan (2,000 mPas); (iii) 0.042% NaF and 0.049% TiF 4 ; (iv) as (iii) with addition of 0.5% chitosan (500 mPas); (v) as (iii) with addition of 0.5% chitosan (2,000 mPas); (vi) commercial solution with SnCl 2 /AmF/NaF (positive control); or (vii) deionized water (negative control). The samples were submitted to pH cycling for 7 d (0.1% citric acid, 4 9 90 s d À1). The treatment was applied once a day for 30 s. The dentin loss was quantified using a contact profilometer. Three samples per group were evaluated using scanning electron microscopy. The dentin loss (lm) was submitted to ANOVA and Tukey's test for differences between treatments. Among the treatments tested, only chitosan 500 mPas was able to statistically significantly reduce the dentin loss compared to the negative control, being similar to the positive control. TiF 4 /NaF, whether with or without chitosan, had no protective effect. Chitosan 500 mPas and SnCl 2 /AmF/NaF solutions have comparable protective effect against dentin erosion in vitro.

The protective effect of the experimental TiF4 and chitosan toothpaste on erosive tooth wear in vitro

“…Taking this idea in mind, it is expected improvement of the protective effect of TiF 4 toothpaste, already tested 17 , with the inclusion of chitosan into the formulae. Recently, we have shown that solutions containing TiF 4 /NaF and chitosan had a protective effect against enamel wear similar to the positive control (Elmex® GABA solution, containing Sn 2+ and F − ) 23 , while for dentin, no improvement in the effect of the fluoridated solution was seen with the addition of chitosan 24 .…”

“…The chitosan toothpaste was prepared as described for the experimental solution 23 , 24 . All toothpastes were diluted (1 part toothpaste to 3 parts deionized water by weight; hereafter named slurry) for the treatment.…”

“…The baseline profile was compared to the final profile for the calculation of the ETW by using the software Marh Surf XCR20. This analysis was done under 100% humidity for dentin 13 , 14 , 23 , 24 .…”

Applications of scanning electron microscopy and focused ion beam milling in dental research

“…Electron microscopy has also proven to be a valuable tool for recent studies of treatments meant to prepare the tooth to bond to resin [81], enhance dental appearance [82], combat and prevent erosion and caries [83][84][85][86][87][88][89][90], or treat sensitivity [64,[91][92][93][94]. Enamel erosion, gum recession from gingivitis, or fractured teeth often result in sensitivity from exposed dentin [95,96].…”

Natural Polymers for the Maintenance of Oral Health: Review of Recent Advances and Perspectives

“…Importantly, this intervention enables avoiding root canal treatment, as an endodontically treated tooth is weaker and more prone to fractures [ 67 ]. It was proven that chitosan could reduce dentine loss when used in the treatment procedure [ 68 ]. Moreover, chitosan–collagen scaffolds have an odontogenic effect and may prevent endodontic treatment due to dentine tissue production [ 69 ].…”