An enzyme-free sensing platform based on molecularly imprinted polymer/MWCNT composite for sub-micromolar-level determination of pyruvic acid as a cancer biomarker

Alizadeh, Taher; Nayeri, Sahar

doi:10.1007/s00216-019-02273-4

Cited by 26 publications

(5 citation statements)

References 46 publications

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“…In the past decade, the number of studies toward using MIPs as a biomarker has increased, as they hold a potential to provide a sensitive, undamaged, rapid and low-cost diagnostic tool [80]. The detection of serum albumin [81,82], hemoglobin [83,84], ferritin [85] and avidin [86], as well as studies of infectious diseases [87][88][89], bone loss [90,91], cardiovascular diseases [92] and various cancers are some areas [4,[93][94][95][96][97][98][99][100][101][102] that are of high interest for the use of MIPs as biomarkers.…”

Section: Mips and Biomarkersmentioning

confidence: 99%

“…Cancer biomarkers have become more compelling over the past decade because they provide early diagnostics, which increases survival. Pyruvic acid, SA, pro gastrin releasing peptide, p53, carbohydrate antigen 15-3, CEA, PSA, EGFR and VEGF are some of the cancer biomarkers that have been studied by using the molecular imprinting technique [4,[93][94][95][96][97][98][99][100][101][102].…”

Section: Mips and Biomarkersmentioning

confidence: 99%

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Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies.

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Section: Mips and Biomarkersmentioning

confidence: 99%

Section: Mips and Biomarkersmentioning

confidence: 99%

Molecularly Imprinted Polymers in Biological Applications

et al. 2020

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“…A deficiency of this causes scurvy [ 194 ]. Ascorbic acid acts as an antioxidant in the body, protecting normal cells from various reactive oxygen species [ 195 ] and helping to maintain the immune system [ 196 ]. In addition, it suppresses various inflammatory reactions [ 197 ] and aging [ 198 ], and helps the elderly to maintain cognitive ability and memory, thereby helping to prevent Alzheimer’s disease [ 199 ].…”

Section: Applications Of Carbon Nanotubes As Biosensorsmentioning

confidence: 99%

Carbon Nanotube and Its Derived Nanomaterials Based High Performance Biosensing Platform

Mondal

Surwase

et al. 2022

Biosensors

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After the COVID-19 pandemic, the development of an accurate diagnosis and monitoring of diseases became a more important issue. In order to fabricate high-performance and sensitive biosensors, many researchers and scientists have used many kinds of nanomaterials such as metal nanoparticles (NPs), metal oxide NPs, quantum dots (QDs), and carbon nanomaterials including graphene and carbon nanotubes (CNTs). Among them, CNTs have been considered important biosensing channel candidates due to their excellent physical properties such as high electrical conductivity, strong mechanical properties, plasmonic properties, and so on. Thus, in this review, CNT-based biosensing systems are introduced and various sensing approaches such as electrochemical, optical, and electrical methods are reported. Moreover, such biosensing platforms showed excellent sensitivity and high selectivity against not only viruses but also virus DNA structures. So, based on the amazing potential of CNTs-based biosensing systems, healthcare and public health can be significantly improved.

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“…At present, a variety of analytic methods are available for the quantification of PA, such as colorimetric analysis, 4 capillary electrophoresis with amperometry detection, 5 voltammetry/polarography, 6 and chromatography 1 . Compared with other methods, gas chromatography/mass spectrometry (GC/MS) has been widely used in the detection of volatile and semi‐volatile compounds because of its sensitivity, specificity, and reliability 7 .…”

Section: Introductionmentioning

confidence: 99%

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Zhao

Zhang

Guo

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses. Methods In GC/MS analyses, the internal standard method (ISM) was employed to overcome the matrix effect during the detection of pyruvic acid (PA) in serum samples from a healthy adult female. The accuracy of the ISM was evaluated by comparing it with the standard addition method (SAM). To employ the ISM‐SAM strategy, correction factors (CFs) were established by combining the ISM and the SAM based on different groups. The CFs were used to normalize data onto the results of subsequent analyses. Results When using the ISM to detect levels of PA, a serious bias is observed, thereby affecting the conclusions reached. In contrast, more reliable data can be obtained after normalizing results by undertaking the ISM‐SAM strategy. The feasibility of this strategy was verified by comparing it with the results of the SAM alone. The ISM‐SAM strategy was successfully applied to quantify the PA levels in healthy people and nephrotic syndrome patients. Conclusions Our results indicated that a false outcome was presented when only the ISM was used to adjust the data, and important information would be missed if the correction strategy was not carried out. Therefore, ISM‐SAM, as an available correction method, should be adapted to improve the reliability of research results.

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An enzyme-free sensing platform based on molecularly imprinted polymer/MWCNT composite for sub-micromolar-level determination of pyruvic acid as a cancer biomarker

Cited by 26 publications

References 46 publications

Molecularly Imprinted Polymers in Biological Applications

Molecularly Imprinted Polymers in Biological Applications

Carbon Nanotube and Its Derived Nanomaterials Based High Performance Biosensing Platform

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

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