A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid
Abstract:Rationale
The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses.
Methods
In GC/MS analyses, the internal standard method (ISM) was employed to overcome th… Show more
“…As a broad-spectrum derivatization reagent, BSTFA may cause other substances in serum to react and cause a matrix effect. [28][29][30] Most carbohydrates exist as isomeric metabolites, and the structure of the derivatized products of the isomeric metabolites is similar in the silylation reaction. We used the same parent and product ions to quantify isomeric metabolites such as mannose and galactose.…”
Carbohydrates are crucial in determining the metabolic alterations that occur in various diseases. The separation and detection of carbohydrates in biological samples are particularly difficult because of their structural similarities....
“…As a broad-spectrum derivatization reagent, BSTFA may cause other substances in serum to react and cause a matrix effect. [28][29][30] Most carbohydrates exist as isomeric metabolites, and the structure of the derivatized products of the isomeric metabolites is similar in the silylation reaction. We used the same parent and product ions to quantify isomeric metabolites such as mannose and galactose.…”
Carbohydrates are crucial in determining the metabolic alterations that occur in various diseases. The separation and detection of carbohydrates in biological samples are particularly difficult because of their structural similarities....
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