The study aims to determine the effect of the implementation of local wisdom-based practicum towards students' attitudes, science literacy and learning outcomes. The research was a quasi-experiment using a Pretest-Posttest Control Group design. The population was 130 second grade high school students in Sumbawa Island, Indonesia. From those, 47 students were selected as the sample. The data related to the students' attitudes, science literacy and learning outcome were gathered from students' responses in questionnaire and multiple choices questions. Data analysis was done quantitatively. The hypothesis testing showed there is a difference between the students' attitudes, science literacy and learning outcome between control and experiment class. In general, the students in experiment class who using local wisdom based-practicum activities attained higher mean scores for every variable. The correlation test also showed a strong relation among the variables. Moreover, the attitudes and science literacy were related and implied to the improvement in learning outcome. To conclude, local wisdom-based practicum influenced the students' attitudes, literacy and learning outcome in science.