AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene Expression in Single- and Co-cultured Bovine Satellite Cells and Intramuscular Preadipocytes Treated with Palmitic, Stearic, Oleic, and Linoleic Acid

Choi, Seong‐Ho; Park, S.K.; Johnson, B. J.; Chung, Ki Yong; Choi, Chang Weon; Kim, K.H.; Kim, W.Y.; Smith, Brennan K.

doi:10.5713/ajas.14.0598

Cited by 30 publications

(25 citation statements)

References 33 publications

(47 reference statements)

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“…In Duckett et al's study, expression of FASN and SCD1 also tended to be down regulated with C18:3 supplementation (Table 2), which would suggest that de novo lipogenesis was not stimulated. In this regard, Choi et al (2015) reported that oleic acid and linoleic acid down-regulated SCD1 expression in bovine subcutaneous and intramuscular preadipocytes. Also, feeding palm oil (high in oleic acid) or soybean oil (high in PUFA), to growing cattle, down-regulated SCD1 expression in subcutaneous adipose tissue (Choi et al, 2016).…”

Section: Marblingmentioning

confidence: 98%

Review: Nutrigenomics of marbling and fatty acid profile in ruminant meat

Ladeira

Schoonmaker

Swanson

et al. 2018

Animal

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The present review will present the recent published results and discuss the main effects of nutrients, mainly fatty acids, on the expression of genes involved in lipid metabolism. In this sense, the review focuses in two phases: prenatal life and finishing phase, showing how nutrients can modulate gene expression affecting marbling and fatty acid profile in meat from ruminants. Adiposity in ruminants starts to be affected by nutrients during prenatal life when maternal nutrition affects the differentiation and proliferation of adipose cells enhancing the marbling potential. Therefore, several fetal programming studies were carried out in the last two decades in order to better understand how nutrients affect long-term expression of genes involved in adipogenesis and lipogenesis. In addition, during the finishing phase, marbling becomes largely dependent on starch digestion and glucose metabolism, being important to create alternatives to increase these metabolic processes, and modulates gene expression. Different lipid sources and their fatty acids may also influence the expression of genes responsible to encode enzymes involved in fat tissue deposition, influencing meat quality. In conclusion, the knowledge shows that gene expression is a metabolic factor affecting marbling and fatty acid profile in ruminant meat and diets and their nutrients have direct effect on how these genes are expressed.

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Section: Marblingmentioning

confidence: 98%

Review: Nutrigenomics of marbling and fatty acid profile in ruminant meat

Ladeira

Schoonmaker

Swanson

et al. 2018

Animal

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“…We recently demonstrated that the SFA palmitic acid (16:0) and stearic acid (18:0) strongly promoted CCAAT enhancer binding protein-beta ( CEBPβ ) and carnitine-palmitoyl-CoA transferase-1beta ( CPT1β ) gene expression in intramuscular (i.m.) preadipocytes ( Choi et al, 2015 ). Conversely, the MUFA oleic acid (18:1 cis -9) and the polyunsaturated fatty acids (PUFA) linoleic (18:2n-6) and α-linolenic acid (18:3n-3) depressed SCD gene expression.…”

Section: Introductionmentioning

confidence: 99%

The Expression of Adipogenic Genes in Adipose Tissues of Feedlot Steers Fed Supplementary Palm Oil or Soybean Oil

Choi

Park

Choi

et al. 2016

Asian Australas. J. Anim. Sci

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We hypothesized that supplementing finishing diets with palm oil would promote adipogenic gene expression and stearoyl-CoA desaturase (SCD) gene expression in subcutaneous (s.c.) and intramuscular (i.m.) adipose tissues of feedlot steers. Eighteen Angus and Angus crossbred steers were assigned to three groups of 6 steers and fed a basal diet (control), with 3% palm oil, or with 3% soybean oil, for 70 d, top-dressed daily. Tailhead s.c. adipose tissue was obtained by biopsy at 14 d before the initiation of dietary treatments and at 35 d of dietary treatments. At slaughter, after 70 d of dietary treatment, tailhead s.c. adipose tissue and i.m. adipose tissue were obtained from the longissimus thoracis muscle. Palm oil increased plasma palmitic acid and soybean oil increased plasma linoleic acid and α-linolenic acid relative to the initial sampling time. Expression of AMP-activated protein kinase alpha (AMPKα) and peroxisome proliferator-activated receptor gamma (PPARγ) increased between the initial and intermediate biopsies and declined thereafter (p<0.03). SCD gene expression did not change between the initial and intermediate biopsies but declined by over 75% by the final period (p = 0.04), and G-coupled protein receptor 43 (GPR43) gene expression was unaffected by diet or time on trial. Soybean oil decreased (p = 0.01) PPARγ gene expression at the intermediate sample time. At the terminal sample time, PPARγ and SCD gene expression was less in i.m. adipose tissue than in s.c. adipose tissue (p<0.05). AMPKα gene expression was less in s.c. adipose tissue of palm oil-fed steers than in control steers (p = 0.04) and CCAAT enhancer binding protein-beta (CEBPβ) gene expression was less in s.c. and i.m. adipose tissues of palm oil-fed steers than in soybean oil-fed steers (p<0.03). Soybean oil decreased SCD gene expression in s.c. adipose tissue (p = 0.05); SCD gene expression in palm oil-fed steers was intermediate between control and soybean oil-fed steers. Contrary to our original hypothesis, palm oil did not promote adipogenic gene expression in s.c. and i.m. adipose tissue.

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“…Fatty acid methyl esters (FAMEs) were prepared as modified methods described by Lepage and Roy (14) and Choi et al (15). Briefly, approximately 3 g of samples were pulverized in liquid nitrogen, homogenized in a Polytron (PT-MR-2100, Switzerland) with chloroform:methanol (2:1, vol/vol), and filtered.…”

Section: Fatty Acid Composition Analysis By Gas Chromatographymentioning

confidence: 99%

Effects of full-fat soybean diet on performance, carcass characteristics,and fatty acid composition of Hanwoo steers

Kim¹,

Lee²,

Park

2016

Turk J Vet Anim Sci

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Introduction Consumer preferences for meat products have recently focused on health-related functionality rather than marbling score in terms of meat quality (1). Due to its beneficial effects in lowering the risk of metabolic disorders including diabetes, arteriosclerosis, and cancer, conjugated linoleic acid (CLA) has been the subject of beef product research (2). CLA is found predominantly in the milk and meat of ruminants and originates from the process of microbial hydrogenation of either C 18:2 (linoleic acid) or C 18:3 (linolenic acid) into C 18:0 (stearic acid) (3). The total amount of CLA in animal products varies greatly depending on what animals eat; supplementation of ruminant rations with vegetable oils rich in linoleic and linolenic acids increases the CLA content in their meat (4). Soybean, the most widely used feed ingredient, is rich in protein and fat and provides the essential amino acids and fatty acids, especially unsaturated fatty acids (5). Enhanced ruminal CLA content was observed through supplementation with either linseed or fish oil (6). Feeding cattle with high-oil corn (7) or soybean oil (8) had no effect on CLA production. Administration of extruded full-fat soybean increased CLA content in the fat tissues of steers (9). Therefore, we hypothesized that full-fat soybean, which is rich in unsaturated fatty acids and highquality amino acids, would increase the CLA content in Hanwoo beef. The objective of this study was to evaluate the effect of a full-fat soybean diet on performance, carcass characteristics, and CLA content of loin muscle in Hanwoo steers. 2. Materials and methods 2.1. Animals, diets, and management The experiment was conducted at an experimental cattle farm in Gyeongbuk, Korea. Experimental animals were handled according to the guidelines of the National Institute of Animal Science, Korea. Thirty Hanwoo steers (BW: 653 ± 52.96 kg, age: 23.7 ± 0.2 months) were stratified by BW and age and randomly assigned to control or full-fat soybean diet groups. Steers were fed using Calan gates for individual intake measurement; diets consisted of a basal corn-based finishing diet (control) or control diet plus 5% full-fat soybean, which was replaced with 3% soybean meal and 2% coconut meal. Steers were kept

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AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene Expression in Single- and Co-cultured Bovine Satellite Cells and Intramuscular Preadipocytes Treated with Palmitic, Stearic, Oleic, and Linoleic Acid

Cited by 30 publications

References 33 publications

Review: Nutrigenomics of marbling and fatty acid profile in ruminant meat

Review: Nutrigenomics of marbling and fatty acid profile in ruminant meat

The Expression of Adipogenic Genes in Adipose Tissues of Feedlot Steers Fed Supplementary Palm Oil or Soybean Oil

Effects of full-fat soybean diet on performance, carcass characteristics,and fatty acid composition of Hanwoo steers

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