Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+

Piano, Martina; Şerban, S.; Pittson, R.; Drago, Guido A.; Hart, John P.

doi:10.1016/j.talanta.2010.03.051

Cited by 52 publications

(23 citation statements)

References 8 publications

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“…By comparing the values obtained in the two types of analysis before physical activity, we can observe a lactate concentration in saliva approximately 10 times lower than the values obtained in the blood. Comparing saliva and blood values after physical activity, the 1:10 relationship between the concentrations is preserved only for athletes 1,9,11,12. The values of other subjects could be caused by some possible causes such as different physical training of athletes participating in the study, but it should also be noted that, being the saliva a biological matrix, some variability can be expected.…”

Section: Analysis Of Saliva and Blood Samplesmentioning

confidence: 94%

“…Another great advantage is the specificity of the immobilized enzyme and the selectivity of the electrochemical transducer, which make the probe suitable for measurements in biological fluids. Numerous amperometric lactate biosensors, based on screen-printing and in which Lactate Dehydrogenases (LDH) or Lactate Oxidases (LOx) were immobilized on their surfaces, have been previously reported [10][11][12][13][14][15][16][17]. This technology has been successfully employed for the fabrication of electrodes in the last decade, allowing mass production of disposable low-cost sensors.…”

Section: Introductionmentioning

confidence: 99%

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Development of a disposable biosensor for lactate monitoring in saliva

Πετρόπουλος

Piermarini

Bernardini

et al. 2016

Sensors and Actuators B: Chemical

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Section: Analysis Of Saliva and Blood Samplesmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of a disposable biosensor for lactate monitoring in saliva

Πετρόπουλος

Piermarini

Bernardini

et al. 2016

Sensors and Actuators B: Chemical

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“…Although several lactate biosensors have been reported in the literature, in most of the cases the practical utility is not fully demonstrated. [45][46][47][48][49][50][51][53][54][55][56][57] Practical application of the biosensor was examined using three human serum samples collected from B. C. Roy Technology Hospital, IIT Kharagpur. The undiluted serum samples were injected into the supporting electrolyte solution and amperometric repose was monitored to quantify the amount of lactate in each sample.…”

Section: Amperometric Biosensing Of Lactatementioning

confidence: 99%

“…The lower value of obtained here implies the strong affinity of the substrate to the enzyme which facilitates the enzymatic reaction 42,43. The performance of our sensor in terms of detection potential is superior to the existing lactate biosensors[44][45][46][47][48][49][50][51][52][53][54][55][56][57] …”

mentioning

confidence: 99%

Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate

Manna

Raj

2016

J. Mater. Chem. B

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Lactate is a byproduct of glycolysis and serum lactate can be used as non-invasive biomarker to risk-stratifying patients in several life-threatening diseases. Herein, we describe the bioelectrocatalytic sensing of lactate using covalently functionalized reduced graphene oxide (rGO)-based material. The development of lactate biosensor involves the covalent functionalization of rGO with p-nitrophenyl moiety, electrochemical generation of surfaceconfined redox mediator and immobilization of L-lactate dehydrogenase (LDH). The covalently functionalized rGO was characterized by XRD, XPS, FTIR, Raman, resistivity and electrochemical measurements. The covalent attachment of nitrophenyl moiety on the basal plane of the carbon network significantly influences the capacitive property of the rGO.The chemically functionalized rGO was electrochemically tuned to generate the redox mediator (rGO-PhNHOH). Electrochemically generated redox couple (PhNHOH/PhNO) exhibits reversible voltammetric response at ~ -0.06 V with a surface coverage of 7.19±0.26 x 10 -9 molcm -2 . The redox couple efficiently mediates the oxidation of NADH at 0.04 V which is ~600 mV less positive potential than the unmodified electrode. The electrode is highly sensitive towards NADH and it could detect as low as 0.4 µM NADH at the potential of 40 mV in neutral pH without any interference from co-existing bioanalytes. Lactate biosensor was developed using the rGO-PhNHOH functional material and lactate dehydrogenase. The surface-confined redox mediator could successfully detect the enzymatically generated NADH at 40 mV. The biosensor is highly sensitive (10.57±0.38 nAµM -1 cm -2 ) and shows linear response up to 90 µM of Lactate. It could detect lactate as low as 2.5 µM without any interference from other analytes. This biosensor has been successfully used to quantify human serum lactate and the results are in excellent agreement with those obtained by the clinical method.

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“…Detection and quantification of lactate using biosensors is of great importance for both, clinical and food analysis [1][2][3][4]. In the case of food chemistry it is useful for the evaluation of the freshness and stability of milk, dairy products, fruits, vegetables, sausages and wines.…”

Section: Introductionmentioning

confidence: 99%

Development of a Carbon Paste Electrode for Lactate Detection Based on Meldola’s Blue Adsorbed on Silica Gel Modified with Niobium Oxide and Lactate Oxidase

et al. 2011

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A reagentless amperometric biosensor sensitive to lactate was developed. The sensor employs a carbon paste electrode modified with lactate oxidase (LOx) and Meldolas Blue (MB) adsorbed on silica gel coated with niobium oxide. The dependence on the biosensor response was investigated in terms of pH, supporting electrolyte, ionic strength, lactate oxidase (LOx) amounts and applied potential. The biosensor showed an excellent operational stability (96 % of the activity was maintained after 150 determinations) and storage stability (allowing measurements for more than 1.5 months, when stored in a refrigerator). The proposed biosensor also presented good sensitivity allowing lactate quantification at levels down to 6.5 10 À7 mol L À1. Moreover, the biosensor showed a good linear response range (from 0.1 to 5.0 mmol L À1 for lactate). Lactate analysis in biological samples such as blood was also performed. The precision of the data obtained by the proposed biosensor showed reliable results for real complex matrices.

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Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+

Cited by 52 publications

References 8 publications

Development of a disposable biosensor for lactate monitoring in saliva

Development of a disposable biosensor for lactate monitoring in saliva

Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate

Development of a Carbon Paste Electrode for Lactate Detection Based on Meldola’s Blue Adsorbed on Silica Gel Modified with Niobium Oxide and Lactate Oxidase

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