Development of a disposable biosensor for lactate monitoring in saliva

Πετρόπουλος, Κωνσταντίνος; Piermarini, S.; Bernardini, Sergio; Palleschi, Giuseppe; Moscone, Danila

doi:10.1016/j.snb.2016.06.068

Cited by 58 publications

(29 citation statements)

References 30 publications

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“…The sensor detects hydrogen peroxide produced by the reaction catalyzed by the lactate oxidase enzyme immobilized onto the electrode surface. Figure 2 a presents the biosensor integrated with portable instrumentation showing a working range from 0.025–0.25 mM and a limit of detection (LOD) of 0.01 mM [ 14 ]. Roda et al reported a 3D printed mini cartridge that can be used to turn a smartphone or tablet in a luminometer.…”

Section: Analytesmentioning

confidence: 99%

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Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

2020

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A recent development in portable biosensors allows rapid, accurate, and on-site detection of biomarkers, which helps to prevent disease spread by the control of sources. Less invasive sample collection is necessary to use portable biosensors in remote environments for accurate on-site diagnostics and testing. For non- or minimally invasive sampling, easily accessible body fluids, such as saliva, sweat, blood, or urine, have been utilized. It is also imperative to find accurate biomarkers to provide better clinical intervention and treatment at the onset of disease. At the same time, these reliable biomarkers can be utilized to monitor the progress of the disease. In this review, we summarize the most recent development of portable biosensors to detect various biomarkers accurately. In addition, we discuss ongoing issues and limitations of the existing systems and methods. Lastly, we present the key requirements of portable biosensors and discuss ideas for functional enhancements.

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Section: Analytesmentioning

confidence: 99%

“… Portable biosensors for salivary diagnostics. ( a ) Schematic representation of SPE-PB-LOx Biosensor [ 14 ]. ( b ) Schematic of immunosensor against S. pyogenes [ 17 ].…”

Section: Figurementioning

confidence: 99%

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

2020

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“…Lactate is present in sweat, blood, saliva, and urine, and it is an important biomarker for a variety of clinical analyses [ 15 , 16 , 17 , 18 , 19 ]. Lactate in food can be important for determining food quality [ 20 , 21 ], and lactate in cell cultures can indicate the cell condition and status [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Wearable Carbon Nanotube-Based Biosensors on Gloves for Lactate

Luo

Shi

et al. 2018

Sensors

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Developing a simple and direct approach for interfacing a sensor and a target analyte is of great interest for fields such as medical diagnosis, threat detection, food quality control, and environmental monitoring. Gloves provide a unique interface for sensing applications. Here, we show for the first time the development of wearable carbon nanotube (CNT)-based amperometric biosensors painted onto gloves as a new sensing platform, used here for the determination of lactate. Three sensor types were studied, configured as: two CNT electrodes; one CNT electrode, and an Ag/AgCl electrode, and two CNT electrodes and an Ag/AgCl electrode. The sensors are constructed by painting the electrodes using CNT or Ag/AgCl inks. By immobilizing lactate oxidase onto the CNT-based working electrodes, the sensors show sensitive detections of lactate. Comparison of sensor performance shows that a combination of CNT and Ag/AgCl is necessary for highly sensitive detection. We anticipate that these findings could open exciting avenues for fundamental studies of wearable bioelectronics, as well as practical applications in fields such as healthcare and defense.

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“…The use of lactate as the oxidizable fuel in biofuel cells allowed for wearable biocatalytic electrodes ,, externally located on skin instead of surgically implanted devices located inside a body. Various externally available biofluids, e. g., sweat, saliva or tears, containing lactate could be considered as potential source of electrical power using lactate‐activated biofuel cells ,. While biocatalytic electrodes aiming at lactate biosensing were extensively studied ,, the lactate‐activated biofuel cells producing electrical power were studied much less , particularly comparing with broadly studied glucose‐based biofuel cells.…”

Section: Introductionmentioning

confidence: 99%

A Biofuel Cell Based on Biocatalytic Reactions of Lactate on Both Anode and Cathode Electrodes – Extracting Electrical Power from Human Sweat

2017

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Electrodes composed of carbon fibers were modified with graphene nano‐sheets in order to increase their surface area and facilitate electrochemical reactions. Electrocatalytic species, such as Meldola's blue (MB) and hemin were immobilized on the graphene surface due to their π‐π stacking and then used for electrocatalytic oxidation of NADH and reduction of H2O2, respectively. Further modification of these electrodes with enzymes producing NADH and H2O2 in situ (lactate dehydrogenase, LDH, and lactate oxidase, LOx, respectively), allowed assembling of a biofuel cell operating in the presence of lactate, oxygen and NAD+. The cathode of the biofuel cell required lactate and O2 for its operation, while the anode operated in the presence of lactate and NAD+. Notably, both bioelectrocatalytic electrodes operated in the presence of lactate, one producing H2O2 in the reaction catalyzed by LOx in the presence of O2, second producing NADH in the reaction catalyzed by LDH in the presence of NAD+. Both reactions were performed in the biofuel cell without separation of the cathodic and anodic solutions and with no need of a membrane. The biofuel cell was tested in solutions mimicking human sweat and then in real human sweat samples, demonstrating substantial power release being able to activate electronic devices.

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Development of a disposable biosensor for lactate monitoring in saliva

Cited by 58 publications

References 30 publications

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

Wearable Carbon Nanotube-Based Biosensors on Gloves for Lactate

A Biofuel Cell Based on Biocatalytic Reactions of Lactate on Both Anode and Cathode Electrodes – Extracting Electrical Power from Human Sweat

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