Development of a Carbon Paste Electrode for Lactate Detection Based on Meldola’s Blue Adsorbed on Silica Gel Modified with Niobium Oxide and Lactate Oxidase

Pereira, Arnaldo César; Kisner, Alexandre; Tarley, César Ricardo Teixeira; Kubota, Lauro T.

doi:10.1002/elan.201000709

Cited by 16 publications

(13 citation statements)

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“…Electroanalysis 2011, 23, No. 12, 2888-2897GOx is "molecularly wired" by the redox polymer has been reported [24,25].…”

Section: à2mentioning

confidence: 97%

“…On the other hand there has been increasing research on lactate biosensors mainly because its determination in blood allows predicting multiple organ failure and death of patient with septic shock. Lactic acidosis is known to accompany decreased tissue oxygenation and drug toxicity, and the analysis of lactate can be used as a preliminary diagnostic for cystic fibrosis, metabolic disorders, thrombosis and physical condition of athletes [15,16]. In addition to the relevance in clinical diagnosis, the determination of glucose and lactate is very important in other areas such as fermentation control and food analysis [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

et al. 2011

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The bioelectrocatalytical properties and kinetic characteristics of new oxidase biosensors based on two different carbosilane dendrimers are described. The best glucose biosensor developed displayed, in an ascorbate interference free work potential interval, a strictly linear range from 0 to 4.0 mM, a detection limit of 40,6 mM and a response time less than 3 s. The lactate biosensor displayed a linear range from 0 to 0.8 mM, a detection limit of 0.73 mM and a response time less than 2 s. The apparent Michaelis-Menten constants were calculated to be 4.39 mM and 2.08 mM respectively, according to Lineweaver-Burk equation.

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“…Electroanalysis 2011, 23, No. 12, 2888-2897GOx is "molecularly wired" by the redox polymer has been reported [24,25].…”

Section: à2mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

et al. 2011

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“…Oxidases have evolved to catalyse the oxidation of many different substrates and are integral to the biological pathways of plants, animals, and bacteria. Among them the flavin adenine dinucleotide (FAD) dependent GOx (Cash and Clark 2010;Li et al 2011;Shan et al 2010) and cholesterol oxidase (ChOx) (Arya et al 2008;Fang et al 2011) as well as flavin mononucleotide (FMN) dependent lactate oxidase (LOx) (Pereira et al 2011;Romero et al 2010) are suitable candidates for the recognition of important physiologic analytes.…”

Section: Introductionmentioning

confidence: 99%

“…Several redox dyes such as methylene green (Upadhyay et al 2009), methylene blue (Wang and Hasebe 2012), meldola blue (Pereira et al 2011), celestine blue (Noorbakhsh et al 2008), phenazine (Liu et al 1997;Ohfuji et al 2004), thionine (Huang et al 2011), azure B (Shan et al 2003), toluidine blue (Yao and Shiu 2007) can be used as electron transfer mediators for oxidases when immobilised on the electrode surfaces. The main drawback of organic dyes-based biosensors is the leaching out of catalytic materials from the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Unsubstituted phenothiazine as a superior water-insoluble mediator for oxidases

Sekretaryova

Vagin

Beni

et al. 2014

Biosensors and Bioelectronics

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The mediation of oxidases: glucose oxidase (GOx), lactate oxidase (LOx) and cholesterol oxidase (ChOx) by a new electron shuttling mediator, unsubstituted phenothiazine (PTZ), was studied. Cyclic voltammetry and rotating-disk electrode measurements in nonaqueous media were used to determine the diffusion characteristics of the mediator and the kinetics of its reaction with GOx, giving a second-order rate constant of 7.6 × 10 3 M -1 s -1 to 2.1 × 10 4 M -1 s -1 for water -acetonitrile solutions containing 5 % to 15 % water. These values are in the range reported for commonly used azine-type mediators, indicating that PTZ is able to function as an efficient mediator. PTZ and GOx, LOx and ChOx were successfully co-immobilized in sol-gel membrane on a screen-printed electrode to construct glucose, lactate and cholesterol biosensors, respectively, which were then optimised in terms of stability and sensitivity. The electrocatalytic oxidation responses showed a dependence on substrate concentration ranging from 0.6 to 32 mM for glucose, from 19 to 565 mM for lactate and from 0.015 to 1.0 mM for cholesterol detection.Oxidation of substrates on the surface of electrodes modified with PTZ and enzyme membrane was investigated with double-step chronoamperometry and the results showed that the PTZ 2 displays excellent electrochemical catalytic activities even when immobilised on the surface of the electrode. Highlights: The kinetics of the electrocatalytic oxidation of glucose with GOx mediated by a new electron shuttling mediator, PTZ, was studied. PTZ delivers superior potential performance among azine-type redox mediators for GOx. PTZ and GOx, LOx and ChOx were successfully co-immobilized in sol-gel membrane on a screen-printed electrode to construct glucose, lactate and cholesterol biosensors, respectively. Second-order rate constants for the reaction between the mediator and variety of oxidases co-immobilised inside a sol-gel membrane were obtained by chronoamperometry.

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“…Historically, methylene blue, one of the representatives of azines, was the first redox mediator of GOx. 82 Several azines such as methylene green 83 , methylene blue 84 , meldola blue 85 , celestine blue 86 , phenazine 87,88 , thionine 89 , azure B 90 , toluidine blue 91 can be used as electron transfer mediators for oxidases when immobilised on the electrode surface. The main drawback of organic dye-based biosensors is the leakage of catalytic material from the electrode surface.…”

Section: Azines As Mediators For Oxidasesmentioning

confidence: 99%

Novel reagentless electrodes for biosensing

Sekretaryova¹

2014

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Analytical chemical information is needed in all areas of human activity including health care, pharmacology, food control and environmental chemistry. Today one of the main challenges in analytical chemistry is the development of methods to perform accurate and sensitive rapid analysis and monitoring of analytes in 'real' samples. Electrochemical biosensors are ideally suited for these applications.Despite the wide application of electrochemical biosensors, they have some limitations. Thus, there is a demand on improvement of biosensor performance together with a necessity of simplification required for their mass production. In this thesis the work is focused on the development of electrochemical sensors with improved performance applicable for mass production, e.g. by screen printing.Biosensors using immobilized oxidases as the bio-recognition element are among the most widely used electrochemical devices. Electrical communication between redox enzymes and electrodes can be established by using natural or synthetic electron carriers as mediators. However, sensors based on soluble electronshuttling redox couples have low operational stability due to the leakage of water-soluble mediators to the solution. We have found a new hydrophobic mediator for oxidases -unsubstituted phenothiazine. Phenothiazine and glucose oxidase, lactate oxidase or cholesterol oxidase were successfully co-immobilized in a sol-gel membrane on a screen-printed electrode to construct glucose, lactate and cholesterol biosensors, respectively. All elaborated biosensors with phenothiazine as a mediator exhibited long-term operational stability. A kinetic study of the mediator has shown that phenothiazine is able to function as an efficient mediator in oxidase-based biosensors.To improve sensitivity of the biosensors and simplify their production we have developed a simple approach for production of graphite microelectrode arrays. Arrays of microband electrodes were produced by screen printing followed by scissor cutting, which enabled the realization of microband arrays at the cut edge. The analytical performance of the system is illustrated by the detection of ascorbic acid through direct oxidation and by detection of glucose using a phenothiazine mediated glucose biosensor. Both systems showed enhanced sensitivity due to improved mass transport. Moreover, the developed approach can be adapted to automated electrode recovery.Finally, two enzyme-based electrocatalytic systems with oxidation and reduction responses, respectively, have been combined into a fuel cell generating a current as an analytical output (a so-called self-powered biosensor). This was possible as a result of the development of the phenothiazine mediated enzyme electrodes, Abstract which enabled the construction of a cholesterol biosensor with self-powered configuration. The biosensor generates a current when analyte (cholesterol) is added to the cell. The biosensor has been applied for whole plasma analysis.All developed concepts in the thesis are compatible with ...

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Development of a Carbon Paste Electrode for Lactate Detection Based on Meldola’s Blue Adsorbed on Silica Gel Modified with Niobium Oxide and Lactate Oxidase

Cited by 16 publications

References 59 publications

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

Unsubstituted phenothiazine as a superior water-insoluble mediator for oxidases

Novel reagentless electrodes for biosensing

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