Amperometric DNA-Peroxidase Sensor for the Detection of Pharmaceutical Preparations

Abstract

Novel DNA-sensor with enzymatic amplification of the signal has been developed on the base of glassy carbon electrode modified with ds-DNA and horseradish peroxidase (HRP). Phenothiazine dyes Methylene Blue and Methylene Green were used as electrochemical markers for the detection of sulfonamide and anthracycline preparations able to interact with DNA. The biosensor signal related to HRP oxidation of the markers depends on the relation between their bonded and readily oxidized forms which depends on the nature and concentration of pharmaceuticals. Sulfonamides diminish surface concentration of MB accessible for HRP reaction whereas anthracyclines release intercalated marker and increase the signal.

Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

“…One of the approaches used with this goal implies the use of electroactive substances interacting with DNA covalently and non-covalently, such as phenothiazine dyes like MB [23]. Thus, Evtugyn et al recently described a novel DNA sensor based on a glassy carbon electrode modified with ds-DNA and HRP and demonstrated that the biosensor signal related to HRP oxidation of MB and methylene green depends on the nature and concentration of the pharmaceuticals added to the reaction medium [25].…”

“…On the other hand, it is also well known that the interaction of DNA with low-molecular weight compounds can be used for the determination of drugs such as nitroimidazole effectors, anticancer drugs, sulfonamides, or anthracyclines [23][24][25][26]. Moreover, the detection of chemicals that can cause irreversible damage to DNA in the cells is considered of great importance, as it often results in mutations that may lead to hereditary or carcinogenic diseases [27,28].…”

Electrocatalytic response of poly(cobalt tetraaminophthalocyanine)/multi-walled carbon nanotubes-Nafion modified electrode toward sulfadiazine in urine

“…Mediators such as rhodium particles deposited polyvinyl pyridine film (Shaidarova et al, 2004), palladium particles deposited polyaniline film (Shaidarova et al, 2006), cobalt phthalocyanine complex (Wang et al, 1988), and ruthenium oxide-ruthenium cyanide composites film (Shaidarova et al, 2008; were successfully used as the electrode modifier for enhancing electron-transfer kinetics and lowering the overvoltage. Modified electrodes such as carbon nanotubes (CNTs)-Nafion modified electrodes (Issac and Kumar, 2009), boron-doped electrodes (Preechaworapun et al, 2006;Souza et al, 2008), metalloporphyrin modified electrodes (Joseph and Kumar, 2010), bismuth film electrodes , and amperometric DNA-peroxidase sensors (Evtugyn et al, 2005) were designed for sulfonamide antibiotics determination. The results demonstrated that electrochemical methods have advantages of high sensitivity, good stability, and promising selectivity.…”

Sulfadiazine-Potentiometric Sensors for Flow and Batch Determinations of Sulfadiazine in Drugs and Biological Fluids

“…They regard specifically capillary electrophoresis with UV, 1 or electrochemical detection, 2 optical methods such as UV 3,4 or visible [5][6][7] spectrophotometries, and amperometry. 8 The United States Pharmacopoeia (USP) proposes a chromatographic separation before UV quantification of SDZ. 9 Other methods for SDZ determination are meant for the analysis of food [10][11][12][13][14][15][16][17][18][19][20] or feeding stuffs, 21 biological fluids, [22][23][24][25][26][27] or environmental samples.…”