Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Loaiza, Óscar A.; Campuzano, Susana; Prada, A. Guzmán-Vázquez de; Pedrero, Marı́a; Pingarrón, José M.

doi:10.1016/j.snb.2008.01.029

Cited by 15 publications

(4 citation statements)

References 34 publications

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“…Amperometric detection of HRP can be carried out by using a variety of different redox-mediators, such as hexacyanoferrates, ferrocene and its derivatives, quinone and redox dyes [21,22]. In this work, three different electrochemical mediators were tested: two ferrocene derivatives (ferrocene monocarboxylic acid and ferrocene methanol) and a quinone-based compound, hydroquinone [23].…”

Section: Electrochemical Characterization Of Hrp Mediatorsmentioning

confidence: 99%

Enzyme-amplified electrochemical hybridization assay based on PNA, LNA and DNA probe-modified micro-magnetic beads

Laschi

Palchetti

Marrazza

et al. 2009

Bioelectrochemistry

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Section: Electrochemical Characterization Of Hrp Mediatorsmentioning

confidence: 99%

Enzyme-amplified electrochemical hybridization assay based on PNA, LNA and DNA probe-modified micro-magnetic beads

Laschi

Palchetti

Marrazza

et al. 2009

Bioelectrochemistry

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“…The stability of such a bond is high, thus, it could be used for designing electrochemical sensors. This approach is used in numerous studies on electrochemical biosensors, often proving to be the best possible with high stability and sensitivity. , The manipulation of complex biomolecular compounds with nanometer precision has critical ramifications for the creation of biomaterial surfaces with exact responses for targeted biosensing. In particular, the development of effective biosensors based on the controlled deposition of biomolecules at the molecular level requires both the understanding and control of the protein/protein and protein/electrode adsorption processes.…”

Section: Introductionmentioning

confidence: 99%

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

et al. 2023

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In this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au−Ti surface have preferential and stronger binding patterns through the carboxyl bond inducing an enhanced surface coverage with CD147. Control of bonding at the surface is essential for oriented receptor assembling and boosted sensitivity. The complex Au−Ti electrode texture along with optimized MPA concentration is a crucial parameter, enabling to reach the detection limit of ca. 3 ng mL −1 . Scanning electrochemical microscopy imaging and quantum molecular modeling were performed to understand the electrochemical performance and specific assembly of MPA displaying a free stereo orientation and not disturbed by direct interactions with closely adjacent receptors. This significantly limits nonspecific interceptor reactions, strongly decreasing the detection of receptor-binding domain proteins by saturation of binding groups. This method has been demonstrated for detecting the SARS virus but can generally be applied to a variety of protein−antigen systems. Moreover, the raster of the pattern can be tuned using various anodizing processes at the titania surfaces.

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“…Additionally, better results were obtained using labelled analytes [ 3 , 5 ] or antibodies [ 6 ] during the analyses. Alkaline phosphatase and horseradish peroxidase (HRP) are often used for antibody (or antigen) labelling, in which the activity was successfully investigated by SECM [ 7 , 8 , 9 ]. HRP is one of the best candidates for labelling of antibodies due to the small size, fast enzymatic reaction (for instance, compared with glucose oxidase), and the possibility to use a wide range of redox mediators (for instance, compared with alkaline phosphatase).…”

Section: Introductionmentioning

confidence: 99%

Scanning Electrochemical Impedance Microscopy in Redox-Competition Mode for the Investigation of Antibodies Labelled with Horseradish Peroxidase

Zinovicius

Morkvėnaitė-Vilkončienė

Ramanavičienė

et al. 2021

Materials

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Scanning electrochemical microscopy enhanced by electrochemical impedance spectroscopy (SEIM) was applied to detect immobilized antibodies labelled with horseradish peroxidase (Ab-HRP). The localized HRP activity was investigated by the SEIM redox competition (RC-SEIM) mode using hydrogen peroxide as a substrate and hexacyanoferrate as a redox mediator. Electrochemical impedance shows to be related to the consumption of hydrogen peroxide at the ultramicroelectrode. For the evaluation of impedimetric results, an equivalent electric circuit was applied with solution resistance, double-layer capacitance, and charge-transfer resistance. These equivalent circuit characteristics depend on the distance between the sample and ultramicroelectrode, and the concentration of substrate. From the gathered data, the charge-transfer resistance appeared to be the parameter describing the behavior of HRP catalyzed reaction as it showed a linear dependence on H2O2 concentration. The RC-SEIM mode suitability for the studying of HRP catalyzed reactions and for the evaluation of Ab-HRP bound to the surface was demonstrated. Additionally, the applicability of RC-SEIM mode for the determination of Ab-HRP affinity bound to the target analyte was discussed.

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Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Cited by 15 publications

References 34 publications

Enzyme-amplified electrochemical hybridization assay based on PNA, LNA and DNA probe-modified micro-magnetic beads

Enzyme-amplified electrochemical hybridization assay based on PNA, LNA and DNA probe-modified micro-magnetic beads

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

Scanning Electrochemical Impedance Microscopy in Redox-Competition Mode for the Investigation of Antibodies Labelled with Horseradish Peroxidase

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