Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum

Feng, Yuyang; Zeng, Da‐Wu; Tong, Yanan; Lu, Xiaoxue; Dun, Guo-dong; Tang, Bin; Zhang, Zhu-jun; Ye, Xin-li; Li, Qian; Xie, Jianping; Mao, Xuhu

doi:10.1371/journal.pone.0215088

Cited by 26 publications

(31 citation statements)

References 20 publications

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“…PRKACB mutations and gene fusion of PRKACB can lead to adrenal, bile duct, liver, and pancreatic cancers [12,13], meanwhile down-regulation of PRKACB expression may be associated with poor survival in patients with non-small cell lung cancer. Makondi et al found that targeting PRKACB may increase the responsiveness of colorectal tumors to irinotecan treatment [28], and Feng et al also found pathological expressions of PRKACB in human colorectal cancer tissues infected with nucleobacteria [29]. Consistent with previous studies [30], our study showed that PRKACB had a significant discrepancy of expression in normal and tumor tissues of colorectal cancer, and we also depicted that down-regulation of PRKACB expression is a risk factor for declining 1, 3, and 5-year survival rates in patients with CRC.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have confirmed that microRNAs can regulate the expression of PRKACB [29,[36][37][38], affect cell proliferation, adhesion, and metastasis, and eventually bring about the development of tumors. Also, studies have found that antisense oligodeoxynucleotides targeted to protein kinase subunits induce growth arrest, apoptosis, and differentiation in a variety of cancer cell lines both in vitro and in vivo [39].…”

Section: Discussionmentioning

confidence: 99%

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Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Yao¹,

Hu²,

Zhang³

et al. 2020

J. Cancer

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Low expressions of PRKACB are related to the occurrence of various human malignancies. However, the prognostic value of PRKACB expression in colorectal cancer (CRC) patients remains controversial. In this analysis, PRKACB expression in CRC tumors was evaluated across the GEO, TCGA, and Oncomine databases, and a PRKACB survival analysis was performed based on the TCGA profile. We detected PRKACB in 7 GEO series (GSE110225, GSE32323, GSE44076, GSE9348, GSE41328, GSE21510, GSE68468) and TCGA spectra (all P <0.05). A meta-analysis performed in the Oncomine database revealed that PRKACB was significantly up-regulated in neoplastic tissues compared to normal tissues (all P <0.05). A Kaplan-Meier analysis demonstrated that lower PRKACB expression in tumors was significantly associated with poorer overall survival (OS) in patients with CRC (P <0.05). A subgroup analysis showed that low expression of PRKACB correlated with poor 1-, 3-, and 5-year OS (all P <0.05). Furthermore, in males (P = 0.0083), whites (P = 0.0463), and non-mucinous adenocarcinoma patients (P = 0.0108), the down-regulation of PRKACB expression was more significant for the OS prognostic value. Conclusion: PRKACB is down-regulated in tumors and associated with worsening OS in CRC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Yao¹,

Hu²,

Zhang³

et al. 2020

J. Cancer

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“…Based on an integrative meta-analysis, CAMK2B was found to be associated with the development of cancer cachexia [56]. Recently, Feng et al [57] assayed 15 colorectal cancer tissues and 10 paracancerous tissues using microarrays and found that CAMK2B was involved in the progression of Fusobacterium nucleatum-induced colorectal cancer. Another study also reported that CAMK2B played an important role in glioblastoma multiforme using bioinformatics analysis on publicly available datasets [58].…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

Wang

Zhang

et al. 2019

Cancer Cell Int

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Background: The aim of this study was to investigate the regulatory network of lncRNAs as competing endogenous RNAs (ceRNA) in bladder urothelial carcinoma (BUC) based on gene expression data derived from The Cancer Genome Atlas (TCGA). Materials and methods: RNA sequence profiles and clinical information from 414 BUC tissues and 19 non-tumor adjacent tissues were downloaded from TCGA. Differentially expressed RNAs derived from BUC and non-tumor adjacent samples were identified using the R package "edgeR". Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed using the "clusterProfiler" package. Gene ontology and protein-protein interaction (PPI) networks were analyzed for the differentially expressed mRNAs using the "STRING" database. The network for the dysregulated lncRNA associated ceRNAs was then constructed for BUC using miRcode, miRTarBase, miRDB, and Tar-getScan. Cox regression analysis was performed to identify independent prognostic RNAs associated with BUC overall survival (OS). Survival analysis for the independent prognostic RNAs within the ceRNA network was calculated using Kaplan-Meier curves. Results: Based on our analysis, a total of 666, 1819 and 157 differentially expressed lncRNAs, mRNAs and miRNAs were identified respectively. The ceRNA network was then constructed and contained 59 lncRNAs, 23 DEmiRNAs, and 52 DEmRNAs. In total, 5 lncRNAs (HCG22, ADAMTS9-AS1, ADAMTS9-AS2, AC078778.1, and AC112721.1), 2 miRNAs (hsa-mir-145 and hsa-mir-141) and 6 mRNAs (ZEB1, TMEM100, MAP1B, DUSP2, JUN, and AIFM3) were found to be related to OS. Two lncRNAs (ADAMTS9-AS1 and ADAMTS9-AS2) and 4 mRNA (DUSP2, JUN, MAP1B, and TMEM100) were validated using GEPIA. Thirty key hub genes were identified using the ranking method of degree. KEGG analysis demonstrated that the majority of the DEmRNAs were involved in pathways associated with cancer. Conclusion: Our findings provide an understanding of the important role of lncRNA-related ceRNAs in BUC. Additional experimental and clinical validations are required to support our findings.

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“…We used online prediction software CircBank, Targetscan, and miRWalk, which resulted in the identification of 6 miRNAs (miR-7-1-3p, miR-1290, miR-219b-3p, miR-4736, miR-6808-5p, and miR-6893-5p) that might be targeted by circ_0060745 ( Figure 3C). Analysis of the GEO dataset GSE122182 24 resulted in the selection of miR-4736 (probe ID: hsa-miR-4736_st) for further study, as the expression of this microRNA was downregulated in 15 CRC samples compared to that in 10 paratumor samples ( Figure 3D and E). Upregulation of miR-4736 suppressed proliferation and metastasis of LOVO and HT29 cells ( Figure 3F and G), and vice versa ( Figure 3H).…”

Section: Microrna 4736 Acted As a Tumor Suppressor In Ht29 And Lovo Cmentioning

confidence: 99%

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Wang

Ren

et al. 2020

OTT

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Purpose: Recent studies have shown that noncoding RNAs (ncRNAs) play essential roles in the development of a number of cancers. Circular RNAs (circRNAs) have been shown to contribute to the progression of colorectal cancer (CRC). Methods: In this study, the expression levels of circular RNA 0060745 (circ_0060745), and microRNA 4736 (miR-4736) were measured using qRT-PCR. Kaplan-Meier survival analysis and receiver operating characteristic (ROC) analysis were used to evaluate the diagnostic value of circ_0060745. Transwell assay and cell counting kit-8 (CCK8) assay were used to determine the metastatic and proliferative capacity of CRC cells. The expression of chromosome segregation one like (CSE1L) was measured using Western blotting and immunohistochemistry (IHC). In addition, RNA pull-down assay and luciferase assay were performed to verify the targeted binding between miR-473,6 and circ_0060745, and between as miR-4736 and CSE1L. Results: We showed that circ_0060745 was upregulated in CRC, and was associated with unfavorable clinicopathological characteristics. We also showed that circ_0060745 acted as an oncogene and promoted CRC cell proliferation and metastasis. Circ_0060745 was primarily located in the cytoplasm. Furthermore, miR-4736 was downregulated in CRC, was a downstream target of circ_0060745, and mediated proliferation and metastasis. We showed that circ_0060745 sequestered miR-4736, which resulted in CRC cell proliferation and metastasis. Finally, we showed that CSE1L, a downstream target of miR-4736, was upregulated in CRC and mediated suppression of proliferation and metastasis in CRC. Conclusion: The results of this study showed that circ_0060745 promoted CRC cell proliferation and metastasis via modulation of miR-4736/CSE1L signaling. The Circ_0060745/miR-4736/CSE1L axis might be a novel target for the treatment of CRC.

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Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum

Cited by 26 publications

References 20 publications

Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

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