&lt;p&gt;Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging&lt;/p&gt;

Wang, Xuebing; Ren, Yanyi; Ma, Siyao; Wang, Shenyu

doi:10.2147/ott.s240642

Cited by 24 publications

(10 citation statements)

References 46 publications

(52 reference statements)

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“…Also, different circRNAs have been implicated in the developing process of CRC. The previous studies have affirmed some oncogenic factors (circ_0060745, circHIPK3) and tumour suppressors (circ_0002138, circDDX17) in CRC 32‐35 . The expression analysis for circ_0007142 in this study indicated that it was abnormally upregulated in CRC samples and cells.…”

Section: Discussionsupporting

confidence: 62%

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Wang

Hongshu

Wei

2021

Eur J Clin Investigation

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Background Ferroptosis is an iron‐dependent and oxidative cell death form. Recent studies suggested that circular RNAs (circRNAs) regulated ferroptosis in tumour cells. Circ_0007142 was identified as a carcinogenic molecule in colorectal cancer (CRC), but its function on ferroptosis in CRC remains unknown. Methods Circ_0007142, microRNA‐874‐3p (miR‐874‐3p) and glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) levels were assayed using the quantitative real‐time polymerase chain reaction (qRT‐PCR). Cell survival and proliferation were measured by Cell Counting Kit‐8 (CCK‐8) assay. Protein detection was performed by Western blot. Cell apoptosis was analysed by flow cytometry. Ferroptosis was assessed by iron accumulation and oxidative stress. Target binding was evaluated by dual‐luciferase reporter assay. In vivo research was conducted by tumour xenograft in mice. Results Circ_0007142 was overexpressed in CRC. After expression inhibition of circ_0007142, proliferation was reduced, while apoptosis and ferroptosis were facilitated in CRC cells. Mechanically, circ_0007142 was found as a miR‐874‐3p sponge and miR‐874‐3p inhibitor eliminated the regulation of si‐circ_0007142 in CRC cells. MiR‐874‐3p targeted GDPD5 and upregulation of GDPD5 reversed the miR‐874‐3p‐triggered tumour inhibition and ferroptosis promotion in CRC cells. Moreover, GDPD5 was regulated by the circ_0007142/miR‐874‐3p axis. Circ_0007142 also affected CRC tumorigenesis in vivo through the regulation of miR‐874‐3p and GDPD5. Conclusion All these findings proved that circ_0007142/miR‐874‐3p/GDPD5 axis regulated tumorigenesis and ferroptosis of CRC cells. Circ_0007142 might be an available marker for ferroptosis in CRC therapy.

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Section: Discussionsupporting

confidence: 62%

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Wang

Hongshu

Wei

2021

Eur J Clin Investigation

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“…Ren et al revealed that hsa_circ_0001178 functions as a ceRNA for miR-382/587/616 to upregulate ZEB1 (a key trigger of EMT), subsequently facilitating the metastatic dissemination of CRC [ 119 ]. In addition, specific circRNAs promote the transcription of their host genes [ 89 ] or protect their homologous mRNAs from miRNA-mediated degradation by inhibiting miRNA activity [ 105 , 133 ]. For example, Wang et al revealed that hsa_circ_0060745, a circRNA derived from exons 9 and 10 of CSE1L, functions as a ceRNA to sequester miR-4736 and promotes the CSE1L-mediated proliferation and metastasis of CRC cells [ 133 ].…”

Section: Circrnas and Crcmentioning

confidence: 99%

“…In addition, specific circRNAs promote the transcription of their host genes [ 89 ] or protect their homologous mRNAs from miRNA-mediated degradation by inhibiting miRNA activity [ 105 , 133 ]. For example, Wang et al revealed that hsa_circ_0060745, a circRNA derived from exons 9 and 10 of CSE1L, functions as a ceRNA to sequester miR-4736 and promotes the CSE1L-mediated proliferation and metastasis of CRC cells [ 133 ]. Lai et al concluded that hsa_circ_0079662 (circHOXA9), a ceRNA that binds hsa-mir-324-5p, upregulates the expression of the target gene HOXA9 and induces oxaliplatin resistance in human colon cancer through the TNF-α pathway [ 105 ].…”

Section: Circrnas and Crcmentioning

confidence: 99%

Comprehensive landscape and future perspectives of circular RNAs in colorectal cancer

et al. 2021

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Colorectal cancer (CRC) is a common hereditary tumor that is often fatal. Its pathogenesis involves multiple genes, including circular RNAs (circRNAs). Notably, circRNAs constitute a new class of noncoding RNAs (ncRNAs) with a covalently closed loop structure and have been characterized as stable, conserved molecules that are abundantly expressed in tissue/development-specific patterns in eukaryotes. Based on accumulating evidence, circRNAs are aberrantly expressed in CRC tissues, cells, exosomes, and blood from patients with CRC. Moreover, numerous circRNAs have been identified as either oncogenes or tumor suppressors that mediate tumorigenesis, metastasis and chemoradiation resistance in CRC. Although the regulatory mechanisms of circRNA biogenesis and functions remain fairly elusive, interesting results have been obtained in studies investigating CRC. In particular, the expression of circRNAs in CRC is comprehensively modulated by multiple factors, such as splicing factors, transcription factors, specific enzymes and cis-acting elements. More importantly, circRNAs exert pivotal effects on CRC through various mechanisms, including acting as miRNA sponges or decoys, interacting with RNA binding proteins, and even translating functional peptides. Finally, circRNAs may serve as promising diagnostic and prognostic biomarkers and potential therapeutic targets in the clinical practice of CRC. In this review, we discuss the dysregulation, functions and clinical significance of circRNAs in CRC and further discuss the molecular mechanisms by which circRNAs exert their functions and how their expression is regulated. Based on this review, we hope to reveal the functions of circRNAs in the initiation and progression of cancer and highlight the future perspectives on strategies targeting circRNAs in cancer research.

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“…CircRNAs are transcripts that are closed covalently and are resistant to exoribonuclease degradation, which are usually more stable than linear transcripts [5]. Aberrant circRNA expression has been discovered in many kinds of cancers such as breast [6], colorectal [7], gastric [8], and lung [9]. For prostate cancer, studies show that circLMTK2 acts as tumor suppressor in PCa through regulating microRNA-183 expression [10].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of circ-TRPS1 suppressed prostatic cancer prognoses by regulating miR-124-3p/EZH2 axis-mediated stemness

Sha

Lei

Han

et al. 2020

Preprint

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Background: Abnormal circular RNA (circRNA) expression correlates with human traits such as many kinds of cancers. Though circRNAs have links to cancer, they have less characterization in metastatic castration-resistant prostate cancer (PCa), which is main reason for PCa mortality.Methods: Therefore, high-throughput sequencing was used for selected circRNA profiles. We performed RT-qPCR to determine circ-TRPS1 expression regarding PCa tissues. We used fluorescence in situ hybridization (FISH) to detect circ-TRPS1 expression and circ-TRPS1 subcellular localization in PCa tissues. circ-TRPS1 expression in PCa cells was selectively regulated. We employed CCK8, transwell assays to monitor the cell proliferation and invasion, respectively. We employed dual-luciferase reporter and RNA pulldown assays to verify the relationship among circ-TRPS1, miR-124-3p, and EZH2. We examined the circ-TRPS1 effects on PCa cell metastasis and proliferation in vivo through a subcutaneous xenograft model as well as an intravenous tail injection model of nude mice.Results: The result showed that circ-TRPS1 was upregulated significantly in high-grade PCa tissues or cell lines. High circ-TRPS1 expression correlated to aggressive PCa phenotypes. Knockdown of circ-TRPS1 suppressed PCa proliferation and metastasis through targeting miR-124-3p/EZH2 axis-mediated stemness in PCa, which was validated by luciferase reporter assays. EZH2 overexpression or miR-124-3p inhibition reversed the inhibition of circ-TRPS1 silencing in PCa cell migration and proliferation by recovering stemness.Conclusion: In summary, data demonstrated that circ-TRPS1 suppressed PCa progression through functioning similar to a miR-124-3p sponge to enhance EZH2 expression and cancer stem-like cell differentiation. Thus, circ-TRPS1 might be a candidate target for PCa treatment.

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<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Cited by 24 publications

References 46 publications

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Circ_0007142 downregulates miR‐874‐3p‐mediated GDPD5 on colorectal cancer cells

Comprehensive landscape and future perspectives of circular RNAs in colorectal cancer

Downregulation of circ-TRPS1 suppressed prostatic cancer prognoses by regulating miR-124-3p/EZH2 axis-mediated stemness

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