Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Yao, Xiaoya; Hu, Weixian; Zhang, Jie; Huang, Chengzhi; Zhao, Haibi; Yao, Xueqing

doi:10.7150/jca.46156

Cited by 13 publications

(22 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The beta subunit of activated cAMP protein kinase encoded by HGNC (PRKACB)/CAFD2/PKACB/PKA C-beta gene is a member of the serine/threonine protein kinase family. Downregulated expression of PRKACB is associated with shorter OS of CRC patients 49 .…”

Section: Discussionmentioning

confidence: 99%

Identification of potential core genes in colorectal carcinoma and key genes in colorectal cancer liver metastasis using bioinformatics analysis

Niu

Gao

2021

Sci Rep

View full text Add to dashboard Cite

Colorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide. Meanwhile, the majority of CRC related deaths results from liver metastasis. Gene expression profile of CRC patients with liver Metastasis was identified using 4 datasets. The data was analyzed using GEO2R tool. GO and KEGG pathway analysis were performed. PPI network of the DEGs between 1 and 2 gene sets was also constructed. The set 1 is named between primary CRC tissues and metastatic CRC tissues. The set 2 is named between primary CRC tissues and normal tissues. Finally, the prognostic value of hub genes was also analyzed. 35 DEGs (set 1) and 142 DEGs (set 2) were identified between CRC liver metastatic cancer patients. The PPI network was constructed using the top 10 set 1 hub genes which included AHSG, SERPINC1, FGA, F2, CP, ITIH2, APOA2, HPX, PLG, HRG and set 2 hub genes which included TIMP1, CXCL1, COL1A2, MMP1, AURKA, UBE2C, CXCL12, TOP2A, ALDH1A1 and PRKACB. Therefore, ITIH2 might represent the potential core gene for colon cancer liver metastasis. COL1A2 behaves as a key gene in colorectal carcinoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of potential core genes in colorectal carcinoma and key genes in colorectal cancer liver metastasis using bioinformatics analysis

Niu

Gao

2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…RGS20 expression in tumor and adjacent normal tissues (ANTs) was compared using R Limma package (version 3.8) ( 24 ). Univariate and multivariate Cox proportional hazard regression, Kaplan-Meier survival analysis and receiver operating characteristic (ROC) curve analysis were performed.…”

Section: Methodsmentioning

confidence: 99%

“…The interaction network among proteins expressed by RGS20-associated genes was established as previously described ( 24 ). The minimum interaction score required was 0.700 (high confidence), and the protein nodes that did not interact with other proteins were deleted.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the Cytoscape software (http://www.cytoscape.org; version 3.7.1) was used to construct the interaction network, and the top 10 hub genes were identified according to the Cytoscape plug-in (degrees ranking of cytoHubba). The co-expression gene network of RCC was analyzed as previously described (24). The cBioPortal database (http://www.cBioPortal.org/) was used to identify the RGS20 co-expressed genes.…”

Section: Wound Healing Assaymentioning

confidence: 99%

See 1 more Smart Citation

Association of RGS20 expression with the progression and prognosis of renal cell carcinoma

et al. 2021

View full text Add to dashboard Cite

Regulator of G protein signaling 20 (RGS20) has been shown to be highly expressed in various types of cancer. The present study aimed to investigate the effects of RGS20 in patients with renal cell carcinoma (RCC) and in RCC cells. Bioinformatics analysis was performed to analyze the role of RGS20 in RCC. Quantitative PCR and western blotting were used to determine the mRNA and protein expression levels of RGS20 in cells, respectively. After RGS20 inhibition, the proliferation, apoptosis, migration and invasiveness of A-498 cells were tested using MTT assay, EdU assay, propidium iodide staining, Annexin V-FITC/PI kit, wound healing assay and Transwell assay. High RGS20 expression was closely associated with the progression and immune infiltration of RCC, and may be considered as an independent indicator of poor prognosis in RCC. After knocking down RGS20, the proliferation, migration and invasiveness of cells were impaired, the cell cycle was arrested at the G 0 /G 1 phase, and the level of apoptosis was increased. In addition, the mRNA expression levels of securin, CDC20 and cyclin B1 were decreased in RGS20-knockdown cells. RGS20 expression was significantly associated with the infiltration level of activated CD4 T cells, type 1 T helper cells and activated dendritic cells. In summary, RGS20 expression was associated with RCC progression and poor prognosis; thus, it may be used to estimate the prognosis of RCC and may serve as a new potential treatment strategy for RCC.

show abstract

“…We screened genes whose absolute value of Spearman correlation coefficient with OXTR expression was greater than 0.3 and uploaded them to the STRING website to analyze PPI, as described previously [ 25 ]. The threshold value is P value < 0.05.…”

Section: Methodsmentioning

confidence: 99%

Prognostic role of oxytocin receptor in colon adenocarcinoma

Sun

Mao

et al. 2021

Open Medicine

View full text Add to dashboard Cite

The oxytocin receptor (OXTR) is directly involved in the pathological mechanisms of multiple cancers, including breast cancer, prostate cancer, and ovarian cancer; however, the role of OXTR in the modulation of colon adenocarcinoma (COAD) growth, metastasis, and clinical prognosis remains to be elucidated. This study used systematic bioinformatics analysis to explore the effects of OXTR on modulating COAD growth and prognosis in patients with COAD. Compared with normal tissues, OXTR mRNA level was higher in COAD tissues, which was associated with tumor progression. Elevated mRNA level of OXTR also indicated a poor prognosis in COAD patients. Furthermore, high mRNA level of OXTR was significantly associated with pathways involved in cell cycle regulation and signal transduction pathways, including the hedgehog, mTOR, TGF-β, and Wnt signaling pathways. OXTR expression was significantly correlated with the infiltration level of type 2T helper cell, central memory CD8 T cell, CD56 bright natural killer cell, activated CD8 T cell, activated B cell, and Type 1T helper cell. Moreover, silencing OXTR inhibited cell proliferation, migration, and invasion, and arrested the cell cycle. In conclusion, high mRNA level of OXTR indicates poor prognosis.

show abstract

Application of cAMP-dependent catalytic subunit β (PRKACB) Low Expression in Predicting Worse Overall Survival: A Potential Therapeutic Target for Colorectal Carcinoma

Cited by 13 publications

References 43 publications

Identification of potential core genes in colorectal carcinoma and key genes in colorectal cancer liver metastasis using bioinformatics analysis

Identification of potential core genes in colorectal carcinoma and key genes in colorectal cancer liver metastasis using bioinformatics analysis

Association of RGS20 expression with the progression and prognosis of renal cell carcinoma

Prognostic role of oxytocin receptor in colon adenocarcinoma

Contact Info

Product

Resources

About